A5017122768- Muscle Physiology and Disorders
- Tissue Engineering and Regenerative Medicine
- Cardiomyopathy and Myosin Studies
- Nutrition and Health in Aging
- Cellular Mechanics and Interactions
- Reproductive Biology and Fertility
- Orthopaedic implants and arthroplasty
- Bone Tissue Engineering Materials
- Cardiac Fibrosis and Remodeling
- Telomeres, Telomerase, and Senescence
- Sperm and Testicular Function
- biodegradable polymer synthesis and properties
- Exercise and Physiological Responses
- Electrospun Nanofibers in Biomedical Applications
- Cardiac Ischemia and Reperfusion
- Free Radicals and Antioxidants
- Neurogenetic and Muscular Disorders Research
- Genetic Neurodegenerative Diseases
- Sexual Differentiation and Disorders
- Antioxidant Activity and Oxidative Stress
National and Kapodistrian University of Athens
2018-2022
Abstract: The central nervous system (CNS) is one of the most metabolically active organs and characterized by elevated oxygen requirements, while it possesses a diminished anti-oxidative action. This can result in impairment susceptible glia neurons, due to production reactive species (ROS), ultimately neurodegeneration. Indeed, oxidative stress key component pathophysiological mechanisms plethora diseases, including neurodegenerative disorders. Iron, additionally involved transportation...
The process of myogenesis gradually deteriorates as the skeletal muscle ages, contributing to mass loss. aim this study is investigate effect senescence/aging on myogenesis, in vitro. A model multiple cell divisions C2C12 myoblasts was used replicate senescence. Control and aged were investigated during i.e., at days 0, 2, 6of differentiation. SA-β-gal activity comet assay markers aging DNA damage. Flow cytometry performed characterize potential differences cycle between control cells....
Background/Aim: Mechanical loading of differentiated myoblasts in vitro may mimic patterns skeletal muscle vivo. However, it is still uncharacterized the conditions that can produce most effective cells9 biological responses, vitro. This study investigated effects different protocols on expression myogenic regulatory factors, anabolic, atrophy and pro-apoptotic factors myotubes. Materials Methods: C2C12 were underwent various stretching by altering their elongation, frequency duration,...
Cardiomyocytes possess the ability to respond mechanical stimuli by adapting their biological functions. This study investigated cellular and molecular events in cardiomyocyte-like H9C2 cells during differentiation as well signalling gene expression responses of differentiated under various stretching protocols vitro. Immunofluorescence was used monitor MyHC structural changes cardiomyoblast differentiation. Moreover, alterations cardiac-specific markers, cell cycle regulatory factors, MRFs,...
Growth hormone (GH) and insulin-like growth factor 1 (IGF-1) have been proposed to play a pivotal role in male infertility due their anabolic effects. The aim of this study was investigate possible associations between seminal plasma levels GH IGF-1 sperm parameters. Fifty men participated study. Semen analysis performed, while cell-free collected following centrifugation. Seminal concentrations were determined by enzyme-linked immunosorbent assay (ELISA). Due the presence asthenozoospermia...
During aging, muscle cell apoptosis increases and myogenesis gradually declines. The impaired myogenic survival potential of the aged skeletal can be ameliorated by its mechanical loading. However, molecular responses cells to loading remain unclear. This study examined effect aged, proliferating, differentiated myoblasts on gene expression signaling associated with their lineage progression survival.Control C2C12 were cultured elastic membranes underwent passive stretching for 12 h at a low...
Abstract Background: Cardiomyocytes are sensitive to mechanical loading, possessing the ability respond stimuli by reprogramming their gene expression. In this study, signaling as well expression responses of myogenic, anabolic, inflammatory, atrophy and pro-apoptotic genes different stretching protocols were examined in differentiated cardiomyocytes. Methods: H9C2 cardiomyoblasts cultured on elastic membranes up 5 th day differentiation (myotubes) then subjected three altering strain,...
Mechanical loading of differentiated myotubes mimics the pattern mature skeletal muscle and alterations in signaling gene expression responses have been reported upon mechanical applied on myotubes. PURPOSE: This study investigated effects terminally myoblasts (myotubes) associated with progression their myogenic lineage. METHODS: C2C12 were cultured elastic membranes up to day 10 differentiation then underwent a passive, cyclic stretching (2.2% elongation, at frequency 0.25Hz, for 12h)....
PURPOSE: The process of myogenesis is gradually declined and cells apoptosis increases with aging. However, mechanical loading aged skeletal muscle can ameliorate its impaired myogenic survival potential. Yet, the molecular responses το are still elusive. This study examined effects differentiated myoblasts (myotubes) on signaling gene expression associated progression their lineage survival. METHODS: C2C12 were cultured for 50 consecutive days (68 cell cycles) in order to acquire aging...
Skeletal muscle can adapt to mechanical loading by changing its mass and overall contractile phenotype via the activation of mechanotransduction intracellular signaling mechanisms. In vitro differentiated myoblasts (myotubes) has been utilized for mimicking conditions skeletal in vivo. PURPOSE: This study investigated effects myotubes on their gene expression responses associated with various aspects cellular function, such as differentiation, hypertrophy apoptosis. METHODS: C2C12 were...
Reperfusion after myocardial infarction (MI) can worsen cardiac tissue damage and in vitro models of hypoxia/reoxygenation (H/R) have been developed to simulate the vivo ischemia/reperfusion injury. Cardiac muscle stem cells used for regeneration MI due their paracrine actions improving cell survival function, while interestingly, mechanical loading may modulate secretome. PURPOSE: The present study investigated cells’ effects H/R, by treating them with secretome mechanically loaded or...