A5008216576- Alzheimer's disease research and treatments
- RNA Research and Splicing
- Neurological Disease Mechanisms and Treatments
- RNA regulation and disease
- Neuroinflammation and Neurodegeneration Mechanisms
- Mitochondrial Function and Pathology
- Advanced Data Storage Technologies
- Neonatal and fetal brain pathology
- Parallel Computing and Optimization Techniques
- Cancer, Hypoxia, and Metabolism
- Trace Elements in Health
- 14-3-3 protein interactions
- Cholinesterase and Neurodegenerative Diseases
- Cancer-related molecular mechanisms research
- Nuclear Physics and Applications
- Eicosanoids and Hypertension Pharmacology
- Metabolomics and Mass Spectrometry Studies
- Ferroptosis and cancer prognosis
- Prion Diseases and Protein Misfolding
- Tea Polyphenols and Effects
- RNA and protein synthesis mechanisms
- Molecular Biology Techniques and Applications
- Chemical Synthesis and Analysis
- Metabolism and Genetic Disorders
- Biochemical effects in animals
Centro de Biología Molecular Severo Ochoa
2022-2024
Universidad Autónoma de Madrid
2019-2024
Abstract Tauopathies, including Alzheimer’s disease (AD) and frontotemporal lobar degeneration with Tau pathology (FTLD-tau), are a group of neurodegenerative disorders characterized by hyperphosphorylation. Post-translational modifications such as phosphorylation truncation have been demonstrated to be an essential step in the molecular pathogenesis these tauopathies. In this work, we demonstrate existence new, human-specific truncated form generated intron 12 retention human neuroblastoma...
BackgroundTau is a microtubule-binding protein encoded by the MAPT gene. Tau essential for several physiological functions and associated with pathological processes, including Alzheimer's disease (AD). Six tau isoforms are typically described in central nervous system, but current research paints more diverse landscape nuanced balance between isoforms. Recent work has generated intron 11 12 retention. This adds to that evidence, proving existence of transcripts retaining 3. Our aim...
W-Tau, a new tau human-specific splicing isoform generated by intron retention, has been recently described. This contains an 18-residue unique sequence corresponding to the translation of retained region 12. In this work, we have described that such 18-amino-acid peptide from 12 can inhibit and β amyloid peptides aggregation under in vitro conditions. inhibitory function is also present smaller fragments peptide.
An increase in tau protein is believed to be necessary for aggregation. However, whether this due increased expression of the endogenous promoter or accumulation proteostasis failure remains uncertain. To analyze GFP under across different ages and within brain areas. We have measured direct Mapt gene promotor by western blot immunofluorescence, means a commercial knock-out mice generated integrating GFP-encoding cDNA into exon 1 gene. Besides, we analyzed MAPT human samples. similar cortex,...
Introduction: Tauopathies, including Alzheimer's disease (AD), are a group of neurodegenerative disorders characterised by Tau hyperphosphorylation. Post-translational modifications such as phosphorylation and truncation have been demonstrated to be an essential step in the molecular pathogenesis tauopathies. Alternative splicing is process which single gene can produce multiple transcripts and, potentially, many different proteins. isoforms generated alternative from gene, MAPT. In this...