A5062152682- Mycotoxins in Agriculture and Food
- Enzyme Production and Characterization
- Electrochemical sensors and biosensors
- Advanced biosensing and bioanalysis techniques
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Indoor Air Quality and Microbial Exposure
- Electrochemical Analysis and Applications
- Pesticide Residue Analysis and Safety
- Microbial Metabolic Engineering and Bioproduction
- Carcinogens and Genotoxicity Assessment
- Environmental DNA in Biodiversity Studies
- Algorithms and Data Compression
- Fungal and yeast genetics research
- Genomics and Phylogenetic Studies
- Glycosylation and Glycoproteins Research
- Analytical chemistry methods development
- bioluminescence and chemiluminescence research
- Advanced Nanomaterials in Catalysis
- Analytical Chemistry and Sensors
- Enzyme-mediated dye degradation
- Monoclonal and Polyclonal Antibodies Research
- Listeria monocytogenes in Food Safety
- Pharmacological Effects and Assays
- Alcohol Consumption and Health Effects
Jinan University
2011-2024
Jiangsu Yonggang Group (China)
2024
Jiangsu University
2013
Key Laboratory of Guangdong Province
2011
Sun Yat-sen University
1998
In this study, a simple but highly sensitive label-free aptasensor based on screen-printed electrode (SPE) was developed for detection of Cd2+. As crucial biorecognizer, the Cd2+ aptamer (issAP08-Cd2+) first screened out using isothermal titration calorimetric method, which showed that equilibrium dissociation constant (KD) binding with issAP08-Cd2+ 2.9 μM. Then, issAP08-Cd2+, is single-stranded DNA 25 nucleotides, modified thiol and assembled gold electrode. After that, electrochemical used...
Abstract Background Pichia pastoris ( Komagataella phaffii ) is a promising production host, but the usage of methanol limits its application in medicine and food industries. Results To improve constitutive expression heterologous proteins P. pastoris, four new potential transcription regulators (Loc1p, Msn2p, Gsm1p, Hot1p) glyceraldehyde triphosphate dehydrogenase promoter (pGAP) were revealed this study by using cellulase E4 as reporter gene. On basis, series strains with knockout or...
Predictions of aflatoxin (AF) in grain at post-harvest can be useful for ensuring the safety stored grain. Versicolorin (Ver) A, a precursor AFB1, serve as an early indicator AF contamination, even when AFs themselves are present undetectable levels. In current research, we developed probabilistic model based on logistic regression and Ver A levels to estimate risk contamination corn. Moisture content, aflatoxigenic fungal load, initial maximum values first three sampling cycles were...
α-Glucosidase (AG) is a bifunctional enzyme, it has capacity to synthesize 2-O-α-d-glucopyranosyl-l-ascorbic acid (AA-2G) from l-ascorbic (L-AA) and low-cost maltose under mild conditions, but can also hydrolyze AA-2G, which leads low synthesis efficiency of AA-2G.This study introduces rational molecular design strategy regulate enzymatic reactions based on inhibiting the formation ground state enzyme-substrate complex. Y215 was analyzed as key amino site affecting affinity AG AA-2G L-AA....
The objective of this study was to evaluate the feasibility predictive monitoring aflatoxin B1 (AFB1) under granary conditions, since mycotoxin contamination stored grain represents an important issue. Using storage test, we investigated relationship between versicolorin A (Ver A, intermediate in AFB1 biosynthesis) levels and aflatoxigenic fungi, their with production. All samples, except for one, were found be contaminated fungi using PCR analyses, while not detectable before test...
Abstract Pollution of heavy metals in agricultural environments is a growing problem to the health world’s human population. Green, low-cost, and efficient detection methods can help control such pollution. In this study, protein biosensor, mApple-D6A3, was built from rice-derived Cd 2+ -binding D6A3 fused with red fluorescent mApple at N-terminus detect contents metals. Fluorescence intensity indicated biosensor’s sensitivity metal ions its more stable under alkaline conditions. mApple-D6A3...