A5008326855- Bacterial Genetics and Biotechnology
- DNA and Nucleic Acid Chemistry
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Heat shock proteins research
- Molecular Sensors and Ion Detection
- Gold and Silver Nanoparticles Synthesis and Applications
- Electrochemical Analysis and Applications
- Bone Metabolism and Diseases
- Nanofabrication and Lithography Techniques
- Protein purification and stability
- Calpain Protease Function and Regulation
- Advanced biosensing and bioanalysis techniques
- Silk-based biomaterials and applications
- Polymer Surface Interaction Studies
- Supramolecular Chemistry and Complexes
- Peptidase Inhibition and Analysis
- Nanoparticles: synthesis and applications
- Escherichia coli research studies
- DNA Repair Mechanisms
- Enzyme Production and Characterization
- Animal health and immunology
- X-ray Diffraction in Crystallography
- Antimicrobial agents and applications
- thermodynamics and calorimetric analyses
Institute for Basic Science
2015-2018
Government of the Republic of Korea
2018
Saha Institute of Nuclear Physics
2012-2018
University of Kalyani
2008-2012
A method for preparation of copper nanoparticles (Cu-NPs) was developed by simple reduction CuCl2 in the presence gelatin as a stabilizer and without applying stringent conditions like purging with nitrogen. The NPs were characterized spectrophotometry, dynamic light scattering, x-ray diffraction, transmission electron microscopy, atomic force microscopy photoelectron spectroscopy. particles about 50-60 nm size highly stable. antibacterial activity this Cu-NP on Gram-negative Escherichia...
We report here a synthetic ion channel developed from shape-persistent porphyrin-based covalent organic cage. The cage was synthesized by employing synthetically economical dynamic chemistry (DCC) approach. selectively transports biologically relevant iodide ions over other inorganic anions dehydration-driven, mechanism as evidenced vesicle-based fluorescence assays and planar lipid bilayer-based single recordings. Furthermore, the appears to facilitate transport across membrane of living...
Artificial transformation of Escherichia coli with plasmid DNA in presence CaCl2 is a widely used technique recombinant technology. However, exact mechanism transfer across cell membranes largely obscure. In this study, measurements both steady state and time-resolved anisotropies fluorescent dye trimethyl ammonium diphenyl hexatriene (TMA-DPH), bound to cellular outer membrane, indicated heat-pulse (0°C→42°C) step the standard procedure had lowered considerably membrane fluidity cells. The...
Papain is the archetype of a broad class cysteine proteases (clan C1A) that contain pro-peptide in zymogen form which required for correct folding and spatio-temporal regulation proteolytic activity initial stages after expression. This study reports X-ray structure thermostable mutant papain at 2.6 Å resolution. The overall structure, particular mature part protease, similar to those other members family. provides an explanation molecular basis maintenance latency by its pro-segment neutral...
A spider with mussel: a supramolecular fiber formed by dragline protein was tuned to have underwater adhesion property genetic fusion of mussel foot protein.
In the standard procedure for artificial transformation of E. coli by plasmid DNA, cellular competence DNA uptake is developed suspending cells in ice-cold CaCl2 (50−100 mM). It believed that helps adsorption to lipopolysaccharide (LPS) molecules on cell surface; however, binding mechanism mostly obscure. this report, we present our findings an in-depth study vitro interaction between and LPS, using different techniques like absorption circular dichroism spectroscopy, isothermal titration...
Greater charge-screening density facilitates additional conformational mobility of hydrated tRNAs on sub-nanosecond timescales, while it renders global structures more stable.
Abstract With increasing addition of Escherichia coli LPS to calf thymus DNA, both dissolved in CaCl 2 , absorption maxima DNA at 260 nm decreased gradually with the appearance isosbastic points ends spectra, which implied some binding between and LPS. Hill plot absorbance data showed that interaction was positive cooperative nature. For any fixed concentration LPS, extent increased as raised from 1.0 100 mM, signifying electrostatic nature interaction, mediated through Ca 2+ ion. Stepwise...
When the cells of Escherichia coli mutant in heat-shock regulator protein sigma-32 were transformed with plasmid pUC19 DNA, transformation efficiency (TRE) of mutant strain was about 10% wild type (wt) strain, indicating role protein(s) process. In order to search for protein(s), when three different E. coli mutants important proteins (DnaK, DnaJ and GroEL) studied, TRE of dnaK dnaJ mutants similar that their wt strain; but groEL drastically less, is, it is almost TRE its wt counterpart. On...