A5084689618- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Plant nutrient uptake and metabolism
- Bacillus and Francisella bacterial research
- Enzyme Production and Characterization
- Amino Acid Enzymes and Metabolism
- Microbial Metabolic Engineering and Bioproduction
- Polyamine Metabolism and Applications
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Weed Control and Herbicide Applications
- Pesticide and Herbicide Environmental Studies
- Phytase and its Applications
- Viral Infections and Outbreaks Research
- Legume Nitrogen Fixing Symbiosis
- Insect Resistance and Genetics
- Entomopathogenic Microorganisms in Pest Control
- Studies on Chitinases and Chitosanases
- Polymer-Based Agricultural Enhancements
- Biochemical and Molecular Research
- Pharmaceutical and Antibiotic Environmental Impacts
- Plant tissue culture and regeneration
- Environmental Toxicology and Ecotoxicology
- Genetically Modified Organisms Research
- Biochemical and biochemical processes
Huazhong Agricultural University
2016-2025
Nematodes are known to be harmful various crops, vegetables, plants and insects. The present study reports that, chitin upregulates the activity of chitinase (20%) nematicidal potential (15%) Pseudomonas aeruginosa. gene (pachi) from P. aeruginosa was cloned, its pachi protein against Caenorhabditis elegans studied. mortality rate induced by increased 6.3-fold when in association with Cry21Aa Bacillus thuringiensis. Pachi efficiently killed C. native state (LC50 = 387.3 ± 31.7 μg/ml), as...
In this study, a novel type of slow-release fertilizer (SRF) was synthesized through lignin-based microcapsules.K 2 HPO 4 and urea were coated by lignin formaldehyde to prepare microcapsules NPK compound SRF.The performance measured determining the contents in water solution soil column leaching.The release amounts N, P K after 5 days 95.39, 95.28 96.75% water, 83.35, 96.62 90.75% soil, respectively.The properties SRF analyzed Fourier transform infrared spectroscopy, thermogravimetry...
The concentration and homeostasis of intracellular phosphate (Pi) are crucial for sustaining cell metabolism growth. During short-term Pi starvation, is maintained relatively constant at the expense vacuolar Pi. After stored exhausted, plant cells induce synthesis acid phosphatase (APase) to recycle from expendable organic (Po). In this study, expression, enzymatic activity subcellular localization ACID PHOSPHATASE 1 (OsACP1) were determined. OsACP1 expression specifically induced in almost...
Glyphosate, a broad spectrum herbicide widely used in agriculture all over the world, inhibits 5-enolpyruvylshikimate-3-phosphate synthase shikimate pathway, and glycine oxidase (GO) has been reported to be able catalyze oxidative deamination of various amines cleave C-N bond glyphosate. Here, an effort improve catalytic activity that was cloned from glyphosate-degrading marine strain Bacillus cereus (BceGO), we bacteriophage T7 lysis-based method for high-throughput screening engineered...
Glyphosate nonselectively inhibits most herbaceous plants by targeting 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). Introducing glyphosate-resistant EPSPS genes into crops imparts herbicide resistance, essential for molecular breeding. In this study, we enhanced the from Halomonas sp. through directed evolution. After two screening rounds, best variant (HoEPSPS-4M) with four mutations (G112A, M155 V, I285F, D326E) exhibited a 22-fold increase in glyphosate resistance (kcat/Km × Ki)...
ABSTRACT The lethal toxin (LeTx) of Bacillus anthracis plays a key role in the pathogenesis anthrax. protective antigen (PA) is primary part anthrax and forms LeTx by combination with factor (LF). Phenylalanine-427 (F427) crucial for PA function. This study was designed to discover potential novel therapeutic agents vaccines done screening mutants that were mutated at F427 residue dominant-negative inhibitory (DNI) phenotype which nontoxic but inhibited toxicity wild-type LeTx. For this,...
ABSTRACT Effective measures for the prophylaxis and treatment of anthrax are still required counteracting threat posed by inhalation anthrax. In this study, we first demonstrated that chimeric protein LFn-PA, created fusing protective antigen (PA)-binding domain lethal factor (LFn) to PA, retained functions respective molecules. On basis observation, attempted develop an antitoxin targets binding (LF) and/or edema (EF) PA transportation LF/EF. Therefore, replaced in LFn-PA with a...