Aim Post-transplant diabetes mellitus (PTDM) is one of the main complications after kidney transplantation. It known that leptin plays an important role in glucose metabolism and mutations receptor gene (LEPR) are responsible for different renal transplant recipients. We aimed to analyse association polymorphisms LEPR with development PTDM these patients.Methods A total 315 recipients were genotyped Lys109Arg, Gln223Arg Lys656Asn polymorphisms. The impact genetic variables together other...

Preclinical studies indicate that arachidonic acid (AA)-derived eicosanoids contribute to hyperglycemia-induced kidney injury. We aimed determine whether plasma and/or urinary levels of dihydroxyeicosatrienoic (DHETs) and 20-hydroxyeicosatetraenoic (20-HETE) acids are associated with diabetic disease (DKD). A total 334 subjects (132 DKD patients 202 non-diabetic individuals) were studied. Plasma 11,12-DHET, 14,15-DHET 20-HETE measured by LC/MS/MS. Urinary concentrations determined...

Genes in the epoxygenase pathway of arachidonic acid metabolism leading to vasoactive eicosanoids, mainly 20-hydroxyeicosatetraenoic (20-HETE) and epoxyeicosatrienoic (EETs) acids, have been related glucose-induced renal damage preclinical reports. We genotyped 1088 diabetic kidney disease (DKD) patients controls for seven polymorphisms five genes (CYP2C8, CYP2J2, CYP4F2, CYP4A11, EPHX2) along this metabolic route evaluated their effect on DKD risk, clinical outcomes, plasma/urine levels...

This review provides insight into the role that obesity, but especially mediators of adipose tissue, have in both renal disease and transplantation. In particular, we focus on functions leptin adiponectin, inflammatory with widely known clinical implications, as well arachidonic acid-derived eicosanoid metabolites. These latter compounds, epoxyeicosatrienoic (EETs) hydroxyeicosatetraenoic (HETEs) acids, which modulate liberation cytokines are generated epoxygenase pathway biotransformation...

Objectives: This study aimed to investigate whether genetic variations in the OPRD1 gene affect psychopathological symptoms and personality dimensions eating disorders (ED) patients and/or contribute ED risk. Methods: The involved 221 female with anorexia nervosa (AN), 88 bulimia (BN) 396 controls. Sixteen tag-SNPs were identified. Psychometric evalu-ations conducted using Symptom Checklist 90 Revised (SCL-90R) Eating Dis-orders Inventory Test-2 (EDI-2). P-values obtained by regression...

Background: Dopamine physiological functions make dopaminergic genes suitable candidates for association studies in eating disorders (ED). A Val158Met polymorphism the catechol-O-methyltransferase (COMT) gene, which is involved dopamine degradation, has been studied relation to ED. Objective: We aimed analyze between this and general psychopathological symptoms that are often coupled these disorders. Method: total of 303 ED patients, diagnosed according DSM-5 criteria, completed SCL-90R...

We aimed to examine whether combined donor/recipient variants in the leptin receptor (LEPR) and adiponectin (ADIPOQ) genes may affect outcomes renal transplantation. A total of 233 donors their corresponding 307 recipients were genotyped for LEPR rs1805094, rs1137100 rs1137101, ADIPOQ rs1501299 rs224176. Combined genetic scores created investigate associations with delayed graft function (DGF), loss estimated glomerular filtration rate (eGFR). Recipients whose carried variant alleles...

Background Diabetic nephropathy (DN) has become the major cause of end-stage kidney disease and is associated to an extremely high cardiovascular (CV) risk.Methods We screened 318 DN patients for 23 SNPs in four glucose transporters (SLC2A1, SLC2A2, SLC5A1 SLC5A2) KCNJ11 ABCC8, which participate insulin secretion. Regression models were utilised identify associations with renal parameters, atherosclerosis measurements CV events. In addition, 506 individuals normal function also genotyped as...

There is a pressing need for more precise biomarkers of chronic kidney disease (CKD). Plasma samples from 820 subjects [231 with CKD, 325 end-stage (ESKD) and 264 controls] were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS) to determine metabolic profile 28 amino acids (AAs) biogenic amines test their value as markers CKD risk progression. The kynurenine/tryptophan ratio showed the strongest correlation estimated glomerular filtration rate values (coefficient =...

Cytochrome P450 (CYP) enzymes metabolize arachidonic acid to vasoactive eicosanoids such as epoxyeicosatrienoic acids (EETs) and 20-Hydroxyeicosatetraenoic (20-HETE), whilst soluble epoxide hydrolase, encoded by the EPHX2 gene, is in charge of EETs degradation. We aimed analyze influence common, functional polymorphisms four genes donor on renal biopsy scores independently assigned pathologists. Additionally, we examined whether this score or presence these SNPs were independent risk factors...

Prostaglandin E2 (PGE2) is a major actor mediating renal injury. We aimed to determine genetic variability in the genes coding for its receptors (PTGER1-4) and study associations with nephrosclerosis risk clinical outcomes. identified 96 tag-SNPs capturing global PTGER1-4 screened 1209 patients controls. The effect of these variants was evaluated by multivariate regression analyses. Two PTGER3 SNPs, rs11209730 rs10399704, remained significant backward elimination model other non-genetic...

Objective: Arachidonic acid metabolism by cytochrome P450 (CYP) epoxygenases leads to epoxyeicosatrienoic acids (EETs), which are eicosanoids with vasodilator and anti-inflammatory properties. We aim determine whether genetic variability in these routes may contribute cardiovascular (CV) risk renal transplant recipients.Methods: In a cohort of 355 patients, we determined the presence two polymorphisms, CYP2C8*3 CYP2J2*7, known affect eicosanoid levels. Associations CV mortality, event-free...

Diabetic kidney disease (DKD) has been pointed out as a prominent cause of chronic and end-stage renal (ESRD). There is genetic predisposition to DKD, although clinically relevant loci are yet be identified. We utilized custom target next-generation sequencing 70-gene panel screen discovery cohort 150 controls, DKD DKD-ESRD patients. Relevant SNPs for the susceptibility clinical evolution were replicated in an independent validation 824 controls A network analysis aiming assess impact...

Nephrosclerosis patients have a high cardiovascular (CV) risk that is very often of more concern than the renal disease itself. We aimed to determine whether variants in phospholipase-related genes, associated with atherosclerosis and CV outcomes general population, could constitute biomarkers nephrosclerosis and/or its risk. screened 1,209 controls for 86 tag-SNPs were identified SCARB1, PLA2G4A, PLA2G7 gene loci. Regression models utilized evaluate their effect on several clinical...

Nephrosclerosis patients are at an exceptionally high cardiovascular (CV) risk. We aimed to determine whether genetic variability represented by 38 tag-SNPs in genes of the cyclooxygenase pathway (PTGS1, PTGS2, PTGES, PTGES2 and PTGES3) leading prostaglandin E2 (PGE2) synthesis, modified CV traits events 493 nephrosclerosis patients. Additionally, we genotyped 716 controls identify risk associations. The addition three variants, namely PTGS2 rs4648268, PTGES3 rs2958155 rs11300958, a...

There is a pressing need for more precise biomarkers of chronic kidney disease (CKD). Plasma samples from 820 subjects [231 with CKD, 325 end-stage (ESKD) and 264 controls] were analyzed by LC-MS/MS to determine metabolic profile 28 aminoacids (AA) biogenic amines test their value as markers CKD risk progression. The Kynurenine/Tryptophan ratio showed the strongest correlation estimated glomerular filtration rate values (coefficient=-0.731, P<0.0001). Models created Orthogonal...

: This study aimed to investigate whether genetic variations in the