Kethmi N. Jayawardhane

ORCID: 0000-0003-4173-2497
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Research Areas
  • Lipid metabolism and biosynthesis
  • Plant nutrient uptake and metabolism
  • Microbial Metabolic Engineering and Bioproduction
  • CRISPR and Genetic Engineering
  • Plant Disease Resistance and Genetics
  • Algal biology and biofuel production
  • Plant Surface Properties and Treatments
  • Plant Molecular Biology Research
  • Polysaccharides and Plant Cell Walls
  • Plant Virus Research Studies
  • Research in Cotton Cultivation
  • Protein Kinase Regulation and GTPase Signaling
  • Nitrogen and Sulfur Effects on Brassica
  • Ruminant Nutrition and Digestive Physiology
  • Fungal Plant Pathogen Control
  • Plant Genetic and Mutation Studies
  • Animal Genetics and Reproduction
  • Photosynthetic Processes and Mechanisms
  • Plant responses to water stress
  • Diet, Metabolism, and Disease
  • Microbial Metabolism and Applications
  • Plant Disease Management Techniques
  • Protein Hydrolysis and Bioactive Peptides
  • Endoplasmic Reticulum Stress and Disease
  • Plant biochemistry and biosynthesis

University of Alberta
2016-2024

GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE (GPAT) genes encode enzymes involved in glycerolipid biosynthesis plants. Ten GPAT homologues have been identified Arabidopsis. GPATs 4-8 shown to be the production of extracellular lipid barrier polyesters. Recently, GPAT9 was reported essential for triacylglycerol (TAG) developing Arabidopsis seeds. The enzymatic properties and possible functions surface lipid, polar TAG non-seed organs, however, not investigated. Here we show that exhibits sn-1...

10.1093/jxb/erw242 article EN cc-by Journal of Experimental Botany 2016-06-20

Polyunsaturated fatty acids (PUFAs) such as α-linolenic acid (ALA, 18:3Δ9cis,12cis,15cis ) have high nutritional and industrial values. In oilseed crops, PUFAs are synthesized on phosphatidylcholine (PC) accumulated in triacylglycerol (TAG). Therefore, exploring the mechanisms that route PC-derived PUFA to TAG is essential for understanding improving production. The seed oil of flax (Linum usitatissimum) enriched ALA, this plant has many lipid biosynthetic enzymes prefer ALA-containing...

10.1074/jbc.ac119.010601 article EN cc-by Journal of Biological Chemistry 2019-09-03

Microalgal oils in the form of triacylglycerols (TAGs) are broadly used as nutritional supplements and biofuels. Diacylglycerol acyltransferase (DGAT) catalyzes final step acyl-CoA-dependent biosynthesis TAG, is considered a key target for manipulating oil production. Although growing number DGAT1s have been identified over-expressed some algal species, detailed structure-function relationship, well improvement DGAT1 performance via protein engineering, remain largely untapped. Here, we...

10.1111/tpj.14708 article EN The Plant Journal 2020-01-28

Rising emissions of anthropogenic greenhouse gases such as carbon dioxide (CO2), nitrous oxide (N2O) and methane (CH4) are a key driver climate change, which is predicted to have myriad detrimental consequences in coming years if not kept check. Given the potency CH4 terms trapping heat atmosphere short term, well fact that ruminant production currently contributes approximately 30% emissions, there an impetus substantially decrease generation ruminant-derived CH4. While various strategies...

10.3390/agriculture12111780 article EN cc-by Agriculture 2022-10-26

Abstract Alfalfa ( Medicago sativa L.) and sainfoin Onobrychis viciifoila Scop.) are two key forage legumes for the western Canadian cattle industry. Despite high protein content, drawbacks to their use exist, including inefficient digestibility energy efficiency in ruminant system, leading economic losses negative environmental impacts. Increasing proportion of lipids diet is known mitigate greenhouse gas emissions; however, above possess only trace quantities shoot tissues used by...

10.1002/csc2.20027 article EN Crop Science 2020-09-22

Clubroot, caused by the obligate parasite Plasmodiophora brassicae, is one of most devastating diseases canola (Brassica napus) in Canada. The identification novel genes that contribute to clubroot resistance important for sustainable management clubroot, as these may be used development resistant cultivars. Phospholipase As (PLAs) play roles plant defense signaling and stress tolerance, thus are attractive targets crop breeding. However, since an allopolyploid has multiple copies each PLA...

10.3389/fpls.2022.799142 article EN cc-by Frontiers in Plant Science 2022-02-18

Abstract Soilborne pathogens can be highly devastating, and clubroot, caused by Plasmodiophora brassicae , is particularly destructive to cruciferous plants. Although many AP2/ERF family transcription factors (TFs) have crucial physiological functions, very little known regarding their functions in the context of soilborne diseases. Here we investigated roles AINTEGUMENTA-LIKE 7 (AIL7), an AIL sub-family TF family, plant immunity against clubroot. Unexpectedly, both AIL7 overexpression...

10.1101/2024.05.22.595381 preprint EN cc-by-nc-nd bioRxiv (Cold Spring Harbor Laboratory) 2024-05-26

SUMMARY Microalgal oils in the form of triacylglycerols (TAGs) are broadly used as nutritional supplements and biofuels. Diacylglycerol acyltransferase (DGAT) catalyzes final step acyl-CoA-dependent biosynthesis TAG is considered a key target for manipulating oil production. Although growing number DGAT1 s have been identified over-expressed some algal species, detailed structure-function relationship, well improvement performance via protein engineering, remain largely untapped. Here, we...

10.1101/2019.12.21.885871 preprint EN bioRxiv (Cold Spring Harbor Laboratory) 2019-12-23
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