A5101994966- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- Insect and Pesticide Research
- Heat shock proteins research
- Physiological and biochemical adaptations
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Cancer-related gene regulation
- Circular RNAs in diseases
- Nitrogen and Sulfur Effects on Brassica
- Machine Learning in Bioinformatics
- Hypothalamic control of reproductive hormones
- ATP Synthase and ATPases Research
- Genomics and Phylogenetic Studies
- Biochemical and Molecular Research
- Retinoids in leukemia and cellular processes
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
- Vector-borne infectious diseases
- Protist diversity and phylogeny
- Biological Research and Disease Studies
- RNA Research and Splicing
- Growth Hormone and Insulin-like Growth Factors
- DNA and Nucleic Acid Chemistry
- Ubiquitin and proteasome pathways
Tianjin Medical University General Hospital
2010-2024
Shanghai Ocean University
2019-2023
Soochow University
2018
First Affiliated Hospital of Soochow University
2018
The First People’s Hospital of Lianyungang
2018
Tianjin Medical University
2010
Columbia University
2008-2010
Augusta University
2007
University of Memphis
2002
DNA sequencing by synthesis (SBS) on a solid surface during polymerase reaction can decipher many sequences in parallel. We report here method that is hybrid between the Sanger dideoxynucleotide terminating and SBS. In this approach, four nucleotides, modified as reversible terminators capping 3'-OH with small moiety so they are still recognized substrates, combined cleavable fluorescent dideoxynucleotides to perform The ratio of two sets nucleotides adjusted extension cycles proceed....
Circular RNAs (circRNAs) act as microRNA (miRNA) sponges that regulate gene expression and are involved in physiological pathological processes. In this study, we evaluated the roles of circRNAs chemoresistance non-small cell lung cancer (NSCLC) to taxol.High-throughput circRNA microarrays were employed investigate profiles parental A549 taxol-resistant A549/Taxol cells. We predicted miRNA targets differentially expressed via prediction software then constructed a circRNA/miRNA network using...
We use methods from Data Mining and Knowledge Discovery to design an algorithm for detecting motifs in protein sequences. The assumes that a motif is constituted by the presence of "good" combination residues appropriate locations motif. attempts compile such good combinations into "pattern dictionary" processing aligned training set dictionary subsequently used detect new Statistical significance detection results are ensured statistically determining various parameters algorithm. Based on...
Kallmann syndrome (KS) consists of idiopathic hypogonadotropic hypogonadism (IHH) and anosmia/hyposmia. Currently, the fibroblast growth factor receptor 1 (FGFR1) gene is only known autosomal dominant cause KS, which also associated with synkinesia, midfacial defects, dental agenesis.Mutations in FGFR1 typically demonstrate reduced penetrance, variable expressivity, until recently have been exclusively identified families anosmia. The purpose this study was to determine whether mutations...
The aim of this study was to the expression Syk gene and methylation in its promoter region lung cancer investigate relationship between silencing DNA cell lines. Real-time PCR immunohistochemistry were used examine specimens from 3 lines 16 patients (tumor tissues adjacent normal tissues). MSP analyze status region. We also investigated role restoring by using a methyltransferase inhibitor, 5-aza-CdR, suppressing invasion No detected In patient samples, significantly lower tumor than that...
We have previously shown that heat shock protein (Hsp70) is significantly up-regulated by grass carp reovirus (GCRV) infection, and Hsp70 plays a positive role in facilitating GCRV infection vitro through unknown mechanisms. Considering the fact most highly induced cells undergoing conserved response (HSR), we determined here both aquareovirus expression of ctenopharyngodon idella kidney cell line (CIK). The pro-viral function was associated with involvement replication transcription complex...
Plant-derived quercetin is a major dietary flavonoid and known mammalian transcriptional repressor for heat shock factor 1 (HSF-1), which responsible the activation of proteins upon stress stimulation. Our previous studies indicated that Hsp70, protein during response, induced to facilitate infection grass carp reovirus (GCRV), could reduce replication efficiency both genotype I III GCRVs in vitro. Here, we provide vivo evidence show effectively increase survival rate rare minnow Gobiocypris...
Translocational regulation of proinsulin biosynthesis in pancreatic β-cells is unknown, although several studies have reported an important accessory role for the Translocon-Associated Protein complex to assist preproinsulin delivery into endoplasmic reticulum via heterotrimeric Sec61 translocon (comprised α, β, and γ subunits). The actual protein-conducting channel α–subunit encoded either by Sec61A1 or its paralog Sec61A2. Although underlying selectivity translocation almost all Sec61α...
To screen efficient siRNA for inhibiting hepatitis B virus using the technique of PCR-based tRNA(val) Pol III-shRNA expression cassettes (SECs).Based on core gene sequence HBV, five target sites were designed. tRNAval produced by one-step overlapping extension PCR strategy co-transfected with HBV C and pC-EGFP plasmid into AD293 cells respectively. Forty-eight hours after transfection, fluorescence HBVC-GFP protein was detected fluorescence-activated cell sorting (FACS); mRNA...
Abstract Phages are proposed for treating and preventing bacteria diseases largely due to their narrow spectrum of activity capacity multiply at the site infection. Aeromonas hydrophila , a gram-negative facultatively anaerobic bacterium, is widely distributed in aquatic environments causing opportunistically almost all freshwater fish displaying various symptoms including septicemia ulcerative infections. In present study, virulent phage A. designated as HJ05, from natural water was...