A5087483143- Wastewater Treatment and Nitrogen Removal
- Advanced biosensing and bioanalysis techniques
- Phosphorus and nutrient management
- Constructed Wetlands for Wastewater Treatment
- Water Treatment and Disinfection
- DNA and Biological Computing
- Berberine and alkaloids research
- Pelvic floor disorders treatments
- Microbial Community Ecology and Physiology
- Microbial Fuel Cells and Bioremediation
- Gastric Cancer Management and Outcomes
- Pharmacogenetics and Drug Metabolism
- HIV/AIDS drug development and treatment
- Angiogenesis and VEGF in Cancer
- RNA Interference and Gene Delivery
- Effects and risks of endocrine disrupting chemicals
- Advanced Nanomaterials in Catalysis
- Ammonia Synthesis and Nitrogen Reduction
- Flavonoids in Medical Research
- Medical Research and Treatments
- Circular RNAs in diseases
- Metastasis and carcinoma case studies
- RNA and protein synthesis mechanisms
- Dental Education, Practice, Research
- Pharmaceutical and Antibiotic Environmental Impacts
Tianjin University of Science and Technology
2022-2025
Tianjin Institute of Industrial Biotechnology
2023-2025
Chinese Academy of Sciences
2023-2025
Weifang Medical University
2025
Tongji University
2023-2024
Chinese Research Academy of Environmental Sciences
2020-2024
Tianjin University of Traditional Chinese Medicine
2022-2024
Annoroad Gene Technology (China)
2024
Henan Cancer Hospital
2008-2023
Zhengzhou University
2006-2023
Template-free enzymatic approaches are considered the most promising solution for next-generation artificial DNA synthesis. However, development of these technologies has been hampered by lack efficient enzymes specialized stepwise nucleotide addition. By combining evolutionary analysis, high-throughput mutagenesis scanning, and rational design, we identified a terminal deoxynucleotidyl transferase from Zonotrichia albicollis (ZaTdT) reshaped its catalytic cavity to better accommodate...
Non-alcoholic fatty liver disease (NAFLD) is a major public health problem in many countries. Berberine (BBR) an effective therapeutic agent alleviating NAFLD. Berberrubine (BRB) one of the main active metabolites BBR, which shows significant anti-obesity and antihypoglycemic effects. However, whether BRB responsible for vivo effect underlying mechanism on NAFLD have not been elucidated. In this study, ability to ameliorate NAFLD, together with its molecular mechanism, was investigated. The...
In the age of information explosion, exponential growth digital data far exceeds capacity current mainstream storage media. DNA is emerging as a promising alternative due to its higher density, longer retention time, and lower power consumption. To date, commercially mature synthesis sequencing technologies allow for writing reading on with customization convenience at research level. However, under disconnected nonspecialized mode, encounters practical challenges, including susceptibility...
Abstract Wire–arc additive manufacturing (WAAM) is acknowledged as a highly effective and economical 3D printing method for large component production repair. However, defects like porosity, oxidization, distortion may occur due to the high deposition rate improper process, leading vital concern about structural quality. The AE signal generated with WAAM process carries wealth of information, which has been proven be an warning evaluating quality printed components. To reveal features from...
Agricultural wastes used as denitrification carbon sources have some drawbacks such excessive organic release and unclear characteristics of nitrogen, phosphorus, chromatic substances, which can cause adverse effects on the effluent quality during process. The composition surface characteristics, mechanisms, secondary pollutant properties six kinds agricultural wastes, i.e., rice straw (RS), wheat (WS), corn stalk (CS), corncob (CC), soybean (SS), hull (SH) were studied analyzed in this...
In recent years, the bacteriophage Φ29 (Phi29) DNA polymerase has garnered increasing attention due to its high-fidelity amplification capacity at constant temperatures. To advance industrial application of this type isothermal polymerases, study mined and characterized new enzymes from microbial metagenome based on known Phi29 sequence. The results revealed that a enzyme, Php29 polymerase, was identified in with plants as hosts. This enzyme exhibited higher strand displacement activity,...
β-galactosidase (β-Gal) has emerged as a pivotal biomarker for the comprehensive investigation of diseases associated with cellular senescence. The development fluorescent sensor is considerable importance precisely detecting activity and spatial distribution β-Gal. In this study, we developed two excited-state-altering responsive sensors (TF1 TF2) ratiometric detection Two TCF dyes, composed tricyanofuran (TCF) naphthol units, feature electron “pull–push” systems are quenched fluorescence...
Pancreatic cancer (PC) is one of the deadliest cancers about digestive system. Recent researches have validated that long non-coding RNAs (lncRNAs) play vital roles in various cancers, while function LINC01006 PC rarely clarified.Investigation specific role PC.LINC01006 expression was examined by RT-qPCR. CCK-8, EdU, transwell, wound healing, and western blot assays were carried out to explore PC. The interaction among LINC01006, miR-2682-5p HOXB8 verified luciferase reporter, RIP ChIP...
Non-nucleoside reverse transcriptase inhibitor (NNRTI)-based highly active antiretroviral therapy (HAART) including efavirenz is recommended as a 1st-line treatment choice in international HIV guidelines, and it one of the most common components initial therapy. Resistance to 1st-generation NNRTIs found among treated untreated HIV-infected individuals creating subpopulation whom not fully effective. This analysis reviewed published articles conference abstracts examine prevalence NNRTI...
Oligonucleotides represent a class of shorter DNA or RNA nucleic acid polymers extensively applied in the biomedical field. Despite progress detecting and analyzing oligonucleotides, high-throughput analysis samples remains challenging. In this work, method for oligonucleotide was developed based on acoustic droplet ejection-open port interface-mass spectrometry (ADE-OPI-MS) technology. This approach to determine enzymatic activity terminal deoxynucleotide transferase (TdT) synthesis, with...
Cdk5/p25 hyperactivity has been demonstrated to lead neuron apoptosis and degenerations. Chronic exposure high glucose (HG) results in of Cdk5 reduced insulin secretion. Here, we set out determine whether abnormal upregulation activity may be induced a pancreatic beta cell line, Min6 cells. We first confirmed that p25 were overexpressed p35 cells treated with HG increased time course dependence. Next, showed no was detected under short stimulation (4–12 hrs), however detectable the long (24...
Hydrilla verticillata–sulfur-based heterotrophic and autotrophic denitrification (HSHAD) process was developed in free water surface constructed wetland mesocosms for the treatment of nitrate-rich agricultural runoff with low chemical oxygen demand/total nitrogen (C/N) ratio, whose feasibility mechanism were extensively studied compared those H. verticillata (HHD) through a 273-day operation. The results showed that can be combined successfully HSHAD mesocosms, achieve satisfactory nitrate...
In this study, temporal and spatial distribution of nitrogen in the Songhua River sediments characteristics related microbes as well relationship between them were investigated.
Xue Anab, Peipei Gaoab, Shasha Zhaoab, Li Zhuab, Xueting Youab, Congyu Lic, Qiurong Zhangab* & Lihong Shanab*a School of Pharmaceutical Sciences Zhengzhou University; b Collaborative Innovation Center New Drug Research and Safety Evaluation, Zhengzhou, PR China; c Affiliated Cancer Hospital University, Henan Hospital, China