A5100655578- PARP inhibition in cancer therapy
- Carcinogens and Genotoxicity Assessment
- Mitochondrial Function and Pathology
- Glutathione Transferases and Polymorphisms
- Prostate Cancer Treatment and Research
- Catalytic C–H Functionalization Methods
- Sulfur-Based Synthesis Techniques
- Genomics, phytochemicals, and oxidative stress
- Cancer Immunotherapy and Biomarkers
- DNA Repair Mechanisms
- Fluorine in Organic Chemistry
- Autophagy in Disease and Therapy
- Liver Disease Diagnosis and Treatment
- Cancer-related gene regulation
- Genetic factors in colorectal cancer
- Neuroscience and Neuropharmacology Research
- Gut microbiota and health
- Toxic Organic Pollutants Impact
- Drug Transport and Resistance Mechanisms
- Cancer, Hypoxia, and Metabolism
- Alzheimer's disease research and treatments
- Adipose Tissue and Metabolism
- Nanoparticle-Based Drug Delivery
- Dietary Effects on Health
- Radical Photochemical Reactions
Nanjing University of Chinese Medicine
2025
Indiana University Bloomington
2013-2024
Lanzhou University
2016-2024
Shandong University
2020-2024
State Key Laboratory of Microbial Technology
2020-2024
Leshan Normal University
2024
Qingdao Binhai University
2020-2023
Peking University
2020-2023
Takeda (United States)
2023
Peking University Cancer Hospital
2023
Glucagon-like peptide 1(7-36) amide (GLP-1) is postulated to be the major physiological incretin in humans, but evidence indirect. We report first studies examining role of GLP-1 postprandial state humans using antagonist exendin 9-39. Exendin 9-39 completely blocked GLP-1-induced glucose-stimulated insulin release from perifused human islets Langerhans. In healthy fasted volunteers, intravenous infusion at 500 pmol x kg(-1) min(-1) hyperglycemic abolished insulinotropic effect a dose and...
Abstract T cell activation requires at least two distinct signals, including signaling via the Ag-specific TCR and a costimulatory pathway. The best characterized pathway involves CD28 molecule, which is expressed constitutively on cells binds family of B7 counter-receptors APCs. Inhibition this prevents can lead to long-term unresponsiveness or anergy. In contrast, CTLA4, homologous CD28, has been shown be negative regulator activation. CTLA4 molecule not resting cells, but induced after...
Glucagon-like peptide-1 7-36 amide (GLP-1) has been postulated to be the primary hormonal mediator of entero-insular axis but evidence indirect. The discovery exendin (9-39), a GLP-1 receptor antagonist, allowed this further investigated. IC50 for binding, using RIN 5AH beta-cell membranes, was found 0.36 nmol/l and 3.44 (9-39). There no competition by (9-39) at binding sites glucagon or related peptides. In anaesthetized fasted rat, insulin release after four doses (0.1, 0.2, 0.3, 0.4...
Mitochondrial injury and dysfunction, a significant feature in metabolic syndrome, triggers endothelial cell dysfunction death. Increasing evidence suggests that mitophagy, process of autophagic turnover damaged mitochondria, maintains mitochondrial integrity. PINK1 (phosphatase tensin homolog (PTEN)-induced putative kinase 1) Parkin signaling is key pathway mitophagy control. In this study, we examined whether could protect mitochondria under stress. We found palmitic acid (PA) induced...
Neurodegenerative diseases are an enormous public health problem, affecting tens of millions people worldwide. Nearly all these characterized by oligomerization and fibrillization neuronal proteins, there is great interest in therapeutic targeting aggregates. Here, we show that soluble aggregates α-synuclein tau bind to plate-immobilized PrP vitro on mouse cortical neurons, this binding requires at least one the same N-terminal sites which Aβ bind. Moreover, tau, cause both functional...
Immune checkpoint inhibitors (ICIs), although promising, have variable benefit in head and neck cancer (HNC). We noted that tumor galectin-1 (Gal1) levels were inversely correlated with treatment response survival patients HNC who treated ICIs. Using multiple mouse models, we show tumor-secreted Gal1 mediates immune evasion by preventing T cell migration into the tumor. Mechanistically, reprograms endothelium to upregulate cell-surface programmed death ligand 1 (PD-L1) galectin-9. genetic...
Social impairment is a defining feature of autism spectrum disorder (ASD) with no demonstrated effective pharmacologic treatments. The goal this study was to evaluate efficacy, safety, and tolerability oral N-acetylcysteine (NAC), an antioxidant whose function overlaps proposed mechanisms ASD pathophysiology, targeting core social in youth ASD. This 12-week randomized, double-blind, placebo-controlled trial NAC Study participants were medically healthy age 4 12 years ASD, weighing ≥15 kg,...
Abstract Single‐cell gel electrophoresis, commonly called a comet assay, is simple and sensitive method for assessing DNA damage at the single‐cell level. It an important technique in genetic toxicological studies. The assay performed under alkaline conditions (pH >13) considered optimal version identifying agents with genotoxic activity. capable of detecting double‐strand breaks, single‐strand alkali‐labile sites, DNA‐DNA/DNA‐protein cross‐linking, incomplete excision repair sites....
Although cardiac cytosolic cyclic 3',5'-adenosine monophosphate (cAMP) regulates multiple processes, such as beating, contractility, metabolism and apoptosis, little is known yet on the role of this second messenger within mitochondria. Using cellular subcellular approaches, we demonstrate here local expression several actors cAMP signaling mitochondria, namely a truncated form soluble AC (sACt) exchange protein directly activated by 1 (Epac1), show protective for sACt against cell death,...
Continuous exposure to various environmental carcinogens and genetic polymorphisms of xenobiotic-metabolizing enzymes (XME) are associated with many types human cancers, including esophageal squamous cell carcinoma (ESCC). Huaian, China, is one the endemic regions ESCC, but fewer studies have been done in characterizing risk factors ESCC this area. The aims study evaluate etiological roles demographic parameters, food-borne exposure, XME formation investigate possible gene-gene...
The mitochondrial transcription factor A (TFAM) is required for DNA (mtDNA) replication and transcription. Disruption of TFAM results in heart failure premature aging mice. But very little known about the role cancer development. Here, we report identification frequent frameshift mutations coding mononucleotide repeat sporadic colorectal (CRC) cell lines primary tumors with microsatellite instability (MSI), but not stable (MSS) CRC tumors. presence truncating mutation, cells MSI, reduced...
Abstract Regulatory T cells (Tregs), a key mediator in regulating anti-tumor immune suppression, tumor escape, metastasis and relapse, are considered an important therapeutic target immunotherapy of human cancers. In the present investigation, elevated CD19 + CD24 CD38 regulatory B (Bregs) were observed PBMCs invasive carcinoma breast (IBCa) patients compared with that fibroadenoma (FIBma) or healthy individuals, positive correlation existed between Bregs CD4 CD25 CD127 − Tregs ( r = 0.316,...
Androgen signaling through androgen receptor (AR) is critical for prostate tumorigenesis. Given that AR-mediated gene regulation enhanced by AR coregulators, inactivation of those coregulators emerging as a promising therapy cancer (PCa). Here, we show the N -acetyltransferase arrest-defect 1 protein (ARD1) functions unique regulator in PCa cells. ARD1 up-regulated human cell lines and primary tumor biopsies. The expression was augmented treatment with synthetic (R1881) unless deficient or...
Perfluorohexanoic acid (PFHxA), a 6-carbon perfluoroalkyl (C6; CAS # 307-24-4), has been proposed as replacement for the commonly used 8-carbon perfluoroalkyls: perfluorooctanoic and perfluorooctane sulfonate. PFHxA is not currently commercial product but rather ultimate degradation of C6 fluorotelomer to make acrylate polymers. It can be expected that, greater or lesser extent, environmental loading will increase, treatments are waste generated. This article reports on chronic study...
Accumulating evidence suggests that B cells play important roles in inhibiting the immune response autoimmune disorders and human tumors as well murine tumor models. In an effort to explore role of breast cancer etiology, we examined presence CD19+ lymphocytes 134 cases invasive carcinoma (IBCa) 31 fibroadenoma, assessed its relationship with PD-L1 (programmed death-ligand 1) expression cancer. We found density was higher IBCa compared significantly associated increasing grade, negative...
Aerobic exercise has been shown to slow tumor progression in rodents and humans, but the mechanisms behind this effect are still unclear. Here we show that aerobic form of chronic endurance training suppresses recruitment FoxP3+ Treg cells thus enhancing antitumor immune efficiency.Adult wild-type athymic BALB/c female mice were endurance-trained for 8 weeks. Circulating leukocytes as well muscle liver mtDNA copy number compared aged-matched concurrent sedentary controls establish systemic...
The activation and functionalization of C–F bonds for the construction C–C C–X have drawn significant research attention in recent years. However, chemo- stereoselective control dual bond gem-difluoroalkenes remains a formidable challenge. Herein, Ni-catalyzed reductive cross-coupling with alkenyl-electrophiles D2O that allowed generation C(sp2)–C(sp2) C(sp2)–D one pot by successive defluorination is described. This methodology offers facile access to various deuterated 1,3-dienes broad...
Designing selective PARP-1 inhibitors has become a new strategy for anticancer drug development. By sequence comparison of and PARP-2, we identified possible site (S site) consisting several different amino acid residues α-5 helix D-loop. Targeting this S site, 140 compounds were designed, synthesized, characterized their activities mechanisms. Compound
Abstract Aberrant activation of the Wnt/β-catenin signaling pathway is a critical event in advanced prostate cancer, but genetic alterations that activate Wnt many other cancers are rarely observed cancer. Other molecular mechanisms regulate cancer remain to be identified. Here, it demonstrated KIF3a, subunit kinesin-II motor protein, functions as an agonist KIF3a upregulated majority human cell lines and primary tumor biopsies. The expression levels correlate with higher Gleason score,...