A5019393845- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Ion channel regulation and function
- Pain Mechanisms and Treatments
- Receptor Mechanisms and Signaling
- Memory and Neural Mechanisms
- Sleep and Wakefulness Research
- Photoreceptor and optogenetics research
- Genetics and Neurodevelopmental Disorders
- Genetic Syndromes and Imprinting
- Nicotinic Acetylcholine Receptors Study
- Adipose Tissue and Metabolism
- Neurotransmitter Receptor Influence on Behavior
- Neuroscience and Neural Engineering
- Genetic Neurodegenerative Diseases
- Neurobiology and Insect Physiology Research
- Ion Channels and Receptors
- Lipid Membrane Structure and Behavior
- Pluripotent Stem Cells Research
- Mitochondrial Function and Pathology
- Erythrocyte Function and Pathophysiology
- Mesenchymal stem cell research
- Muscle Physiology and Disorders
- Olfactory and Sensory Function Studies
- Stress Responses and Cortisol
National Taiwan University
2014-2024
GTx (United States)
2020
Chung Shan Medical University
2020
Taipei Tzu Chi Hospital
2020
Institute of Zoology
2016
Institute of Cognitive and Brain Sciences
2011
Rutgers New Jersey Medical School
2008
Rutgers, The State University of New Jersey
2008
China Medical University
2002-2004
University College London
1998-1999
Acid-sensing ion channel 3 (ASIC3) is involved in acid nociception, but its possible role neurosensory mechanotransduction disputed. We report here the generation of Asic3-knockout/eGFPf-knockin mice and subsequent characterization heterogeneous expression ASIC3 dorsal root ganglion (DRG). expressed parvalbumin (Pv+) proprioceptor axons innervating muscle spindles. further generate a floxed allele Asic3 (Asic3(f/f)) probe neurite-bearing Pv+ DRG neurons through localized elastic matrix...
The subthalamic nucleus (STN) plays a critical role in modulating motor and cognitive functions within the basal ganglia, with its dysfunction being implicated movement disorders such as Parkinson's disease. However, behavioral representations of individual STN neurons remain incompletely understood. Using vivo two-photon calcium imaging behaving mice, we systematically mapped activity single across diverse contexts, including locomotion, licking, reward-driven actions. Our findings reveal...
Exogenous application of agonists at the kainate subtype glutamate receptors has been shown to depress evoked monosynaptic inhibition by γ-aminobutyric acid (GABA)ergic interneurons in hippocampus. This observation led hypothesis that synaptic release endogenous might have a disinhibitory effect on neuronal circuits, addition depolarizing neurons via postsynaptic α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic (AMPA), kainate, and N -methyl- d -aspartic (NMDA) receptors. It is not known,...
The objective of this study was to evaluate the role beta-adrenergic receptors in modulating associative long-term potentiation (LTP) induced at CA1 synapses. Two independent Schaffer collateral pathways were stimulated hippocampal slices. field EPSP (fEPSP) response evoked one pathway (the weak pathway) small, whereas a large response, usually 80-90% maximum, strong pathway. After recording baseline fEPSP 0.033 Hz, LTP could be associatively by paired stimulation and 100 times 6 sec...
Application of phorbol 12,13-diacetate (PDA) caused marked enhancement synaptic transmission nociceptive parabrachio-amygdaloid (PBA) input onto neurons the capsular central amygdaloid (CeAC) nucleus. The potentiation PBA–CeAC EPSCs by PDA involved a presynaptic protein kinase C (PKC)-dependent component and postsynaptic PKC–extracellular-regulated (ERK)-dependent component. NMDA glutamatergic receptor (NMDAR)-dependent long-term (LTP) EPSCs, which was also dependent on PKC–ERK signaling...
Among all voltage-gated calcium channels, the T-type Ca2+ channels encoded by Cav3.2 genes are highly expressed in hippocampus, which is associated with contextual, temporal and spatial learning memory. However, specific involvement of channel these hippocampus-dependent types memory remains unclear. To investigate functional role this memory, we subjected homozygous heterozygous knockout mice their wild-type littermates to behavioral tasks, including trace fear conditioning, Morris...
Sensing external stimulation is crucial for central processing in the brain and subsequent behavioral expression. Although sensory alteration or deprivation may result changes, most studies related to control of behavior have focused on mechanisms. Here we created a deficit model mice lacking acid-sensing ion channel 3 (Asic3(-/-)) probe alterations. ASIC3 predominately distributed peripheral nervous system. RT-PCR immunohistochemistry used examine expression Asic3 mouse showed...
Peroxisomal proliferator-activated receptor gamma (PPARγ) is a nuclear hormone whose agonist, rosiglitazone has neuroprotective effect to hippocampal neurons in pilocarpine-induced seizures. Hippocampal slice preparations treated Mg2+ free medium can induce ictal and interictal-like epileptiform discharges, which regarded as an vitro model of N-methyl-D-aspartate (NMDA) receptor-mediated temporal lobe epilepsy (TLE). We applied slices medium. The effects on CA1-Schaffer collateral synaptic...
The locus coeruleus (LC) contains noradrenergic (NA) neurons that respond to novel stimuli in the environment with phasic activation initiate an orienting response; LC is also triggered by stimuli, representing outcome of task-related decision processes, facilitate ensuing behaviours and help optimize task performance. Here, we report LC-NA exhibit bursts action potentials vitro resembling vivo, activity gated inhibitory interneurons (I-INs) located peri-LC. We observe inhibition peri-LC...
Long-term potentiation (LTP) of excitatory transmission is an important candidate cellular mechanism for the storage memories in mammalian brain. The subcellular phenomena that underlie persistent increase synaptic strength, however, are incompletely understood. A potentially powerful method to detect a presynaptic glutamate release examine effect LTP induction on rate at which use-dependent blocker MK-801 attenuates successive N -methyl- d -aspartic acid (NMDA) receptor-mediated signals....
Abstract Pain-related diseases are the top leading causes of life disability. Identifying brain regions involved in persistent neuronal changes will provide new insights for developing efficient chronic pain treatment. Here, we showed that anterior nucleus paraventricular thalamus (PVA) plays an essential role development mechanical hyperalgesia neuropathic and inflammatory models mice. Increase c-Fos, phosphorylated extracellular signal–regulated kinase, hyperexcitability PVA neurons were...
The locus coeruleus (LC) contains predominantly norepinephrine (NE) neurons with widespread axonal projections throughout the brain and plays a critical role in control of behavior, particularly arousal. Historically, LC-NE system was thought to perform this function by releasing NE uniformly most regions. However, recent evidence suggests that cortical LC are modularly organized, allowing coordination diverse sometimes opposing functions, such as fear memory formation extinction. many...
Abstract The object of the present study is to investigate role endogenous adrenergic innervation in regulating bi‐directional synaptic plasticity rat hippocampal CA1 synapses. system was eliminated by giving subcutaneous injection 6‐hydroxydopamine (6‐OHDA) rats immediately after birth, and animals were killed for experiments at postnatal ages 25–35 days. In slices taken from 6‐OHDA‐treated animals, theta‐burst stimulation 100 Hz failed induce long‐term potentiation (LTP) However, induction...
Aromatic L-amino acid decarboxylase (AADC) deficiency is a rare pediatric neuro-metabolic disease in children. Due to the lack of an animal model, its pathogenetic mechanism poorly understood. To study role AADC brain development, zebrafish model was generated. We identified aadc gene homolog, dopa (ddc), genome. Whole-mount situ hybridization analysis showed that ddc expressed epiphysis, locus caeruleus, diencephalic catecholaminergic clusters, and raphe nuclei 36-h post-fertilization (hpf)...
Abstract The brainstem region, locus coeruleus (LC), has been remarkably conserved across vertebrates. Evolution woven the LC into wide-ranging neural circuits that influence functions as broad autonomic systems, stress response, nociception, sleep, and high-level cognition among others. Given this conservation, there is a strong possibility activity inherently similar species, furthermore age, sex, brain state similarly species. degree to which homogenous these factors, however, never...
Abstract We examined the mechanisms underlying spike‐timing‐dependent plasticity induction at resting and conditioned lateral perforant pathway (LPP) synapses in rat dentate gyrus. Two stimulating electrodes were placed outer third of molecular layer granule cell hippocampal slices to evoke field excitatory postsynaptic potentials (fEPSPs) antidromic somatic spikes (afSSs), respectively. Long‐term potentiation (LTP) LPP was induced by paired stimulation with fEPSP preceding afSS. Reversal...
Key points Noradrenaline (NA)‐releasing neurons in the locus coeruleus (LC) provide NA to forebrain and play important roles regulating many brain functions. LC are subject tonic inhibition mediated by GABA B receptors (GABA Rs) that extent of effect varies with ambient levels. R‐mediated can effectively tune spontaneous firing rate (SFR) neurons; it is developmentally regulated responsible for maintaining a constant SFR during development. In male, but not female rats, chronic perinatal...
Tian ma (Gastrodia elata, GE) is an ancient Chinese herbal medicine that has been suggested to be effective as anticonvulsant and analgesic, have sedative effects against vertigo, general paralysis, epilepsy tetanus. The primary active ingredient isolated from GE termed gastrodin, which the glucoside of 4-hydroxybenzyl alcohol (4-HBA). Gastrodin can abolish hypoxia-, glutamate- N-methyl-D-aspartate (NMDA) receptor-induced toxicity in culture rat cortical neurons, reduces seizure severity...
Spinocerebellar ataxia type 1 (SCA1) is an autosomal dominant neurodegenerative disorder caused by the expansion of CAG repeats in ATXN1 gene resulting polyglutamine protein. Unfortunately, there has yet been any effective treatment so far for SCA1. This study investigated feasibility transplanting human umbilical mesenchymal stem cells (HUMSCs) into transgenic SCA1 mice containing expanded uninterrupted allele with 82 ATXN1-coding region. 106 were transplanted cerebella at month age. HUMSCs...
Relay neurons in the ventrobasal nucleus of thalamus transmit somatosensory information to cerebral cortex and receive sensory cortical (feedback) synaptic inputs via, respectively, medial lemniscal (ML) corticothalamic (CT) fibres. Here, we report that calcium-permeable AMPA receptors are expressed at CT synapses, but not ML NMDA receptor (NMDAR)-mediated/non-NMDAR-mediated current ratio is significantly larger synapses than synapses. Moreover, NMDAR-dependent LTP L-type voltage-gated...