A5086363233- Anatomy and Medical Technology
- Innovations in Medical Education
- Lysosomal Storage Disorders Research
- Surgical Simulation and Training
- Virus-based gene therapy research
- Cellular transport and secretion
- Medical and Biological Sciences
- Molecular Biology Techniques and Applications
- Circadian rhythm and melatonin
- Skin and Cellular Biology Research
- Genetic and Kidney Cyst Diseases
- Vascular anomalies and interventions
- Aortic aneurysm repair treatments
- RNA regulation and disease
- Pharmacological Effects of Medicinal Plants
- Reproductive Biology and Fertility
- Connective tissue disorders research
- Adipose Tissue and Metabolism
- Central Venous Catheters and Hemodialysis
- Renal and Vascular Pathologies
- Trypanosoma species research and implications
- Axon Guidance and Neuronal Signaling
- Point processes and geometric inequalities
- Musculoskeletal Disorders and Rehabilitation
- Olfactory and Sensory Function Studies
University of Massachusetts Chan Medical School
2016-2023
Boston University
2012-2017
Agricultural Research Service
2017
Cornell University
2002-2005
We have previously shown that myelin abnormalities characterize the normal aging process of brain and an age-associated reduction in Klotho is conserved across species. Predominantly generated kidney, overexpression extends life span, whereas loss accelerates development aging-like phenotypes. Although function unknown, expression leads to cognitive deficits. found significant effects on oligodendrocyte functions, including induced maturation rat primary oligodendrocytic progenitor cells...
Mild age-related declines in visual function occur humans and monkeys, independent of ocular pathology, suggesting involvement central pathways (Spear [1993] Vision Res 33:2589-2609). Although many factors might account for this decline, a loss neurons primary cortex (V1) could be contributing factor. Previous studies neuron numbers V1 reported stability across age, but were limited the ages genders studied sampled only parts or cell types, allowing possibility subtle neurons. We pursued...
Storage of tissue sections for long periods allows multiple samples, acquired over months or years, to be processed together, in the same reagents, quantitative histochemical studies. Protocols freezer storage free-floating frozen using sucrose with different additives have been reported and assert that has no effect on histochemistry, but support provided. The present study analyzed efficacy long-term brain at −80C buffered 15% glycerol. To determine whether reactivity is affected, we 11...
Late infantile neuronal ceroid lipofuscinosis (LINCL), a pediatric autosomal recessive neurodegenerative lysosomal storage disorder, results from mutations in the CLN2 gene and consequent deficiency tripeptidyl-peptidase I (TPP-I) progressive destruction of neurons. We have previously demonstrated that CNS transfer AAV2CUhCLN2 (an AAV2-based vector expressing human cDNA) rats nonhuman primates mediates long-term TPP-I expression neurons [Sondhi, D., Peterson, D.A., Giannaris, E.L., Sanders,...
Mice with targeted gene disruption have provided important information about the molecular mechanisms of circadian clock function. A full understanding roles circadian-relevant genes requires manipulation their expression in a tissue-specific manner, ideally including high efficiency within suprachiasmatic nuclei (SCN). To date, conditional SCN has been difficult. In previously developed mouse line, Cre recombinase was inserted into vesicular GABA transporter (Vgat) locus. Since virtually...
Dissection provides a unique opportunity to integrate anatomical and clinical education. Commonly, cadavers are randomly assigned courses, which may result in skewed representation of patient populations. The primary aim this study was determine if the donors studied by students at University Massachusetts Medical School (UMMS) accurately represent disease burden local population. This cross‐sectional compared Memorial Center claims data body donation from UMMS Anatomical Gift Program (AGP)....
We describe a unique solitary kidney with duplex collecting system and vascular variation observed in an 86-year-old white male formaldehyde- phenol-fixed cadaver during routine academic dissection. The left renal fossa was empty intact adrenal gland, the right contained fused mass apparent polarity between superior inferior regions two pelves converging into single ureter. There were three arteries supplying mass; middle noted to be postcaval artery precaval. also veins draining vena cava...
Storage of tissue sections for significant periods is often required to allow from multiple samples, acquired over months years, be processed together, in the same reagents, quantitative studies using immunohistochemistry. Several protocols storage sucrose with different additives have been reported that assert there no effect on immunogenicity. However, assessments how long‐term frozen any cryoprotectant affects The present study analyzed existing datasets, derived monkey brain stored at...
There has been a recent shift in medical student anatomy education with greater incorporation of virtual resources. Multiple approaches to resources have described, but few involve video or images from surgical procedures. In this pilot study, series case videos was created using robotic surgery footage for first-year course. Five operations were included that covered thoracic, abdominal, and pelvic anatomy. Students surveyed at the end course regarding their experience perceptions towards...
The Summer Anatomy Enrichment Program (SAEP) was developed for rising second year medical students at the University of Massachusetts Medical School. goals SAEP are (1) to provide participants an advanced review and further training in anatomy through human cadaveric dissection; (2) develop educational materials component interdisciplinary Development, Structure, Function (DSF) course first students. This selective program is geared toward high achieving anatomical sciences with a strong...
We report varicosities affecting the right deep femoral vein and consequent to a unique unilateral variation in course of small saphenous vein. The vein, instead draining into popliteal at fossa, expanded considerably girth thickness continued as then pierced adductor magnus muscle, appeared anterior compartment, joined Four centimeters distal this junction, two-centimeter long varicosity was noted. There also one-centimeter on its point attachment abnormal has several clinical implications,...
Late infantile neuronal ceroid lipofuscinosis (LINCL), a fatal lysosomal storage disease leading to neurodegeneration, results from mutations in the CLN2 gene which lead deficiency of product, tripeptidyl peptidase I (TPP-I). The objective this study was assess feasibility using therapy interrupt progression LINCL. Due chronic and diffuse nature disease, long term TPP-I production is required throughout brain following transfer. In study, AAV2CUhCLN2, serotype 2 replication-deficient...
Late infantile neuronal ceroid lipofuscinosis (LINCL), a pediatric neurodegenerative lysosomal storage disorder, results from mutations in the CLN2 gene that lead to deficiency tripeptidyl peptidase (TPP-I) and consequent progressive destruction of neurons. Studies our laboratory have demonstrated long term TPP-I expression CNS following transfer AAV2CUhCLN2 (an AAV2-based vector expressing human cDNA) rat non-human primate. To prepare for possible clinical trial, present study tests...
Introduction In the era of radical medical curriculum reform, preclinical anatomy should not only prepare students for USMLE Step 1, but also provide sufficient knowledge clinical clerkships and electives. Unfortunately, data regarding anatomical considered essential any given clerkship or elective is lacking. Aim This IRB study addresses lack on required electives using an online survey provided to clerkship/elective educators evaluate importance 98 items (tissues structures) across all...
Introduction To prepare medical students for clinical training and practice, it is critical to understand the anatomical knowledge considered most important different specialties. Aim address this issue, a consortium of anatomists in US Canada collecting data from educators Internal Medicine clerkships electives identify anatomy they consider essential. Methods An IRB‐approved, online survey (Qualtrics, Seattle, WA) was used assess importance 98 topics seven body regions. The study first...
Introduction To prepare medical students for clinical training and practice, it is critical to understand the anatomical knowledge considered most important different specialties. Aim address this issue, a consortium of anatomists in US Canada collecting data from educators clerkships electives identify anatomy they consider essential their specialty. Methods An IRB‐approved, online survey (Qualtrics, Seattle, WA) was used assess importance 98 topics seven body regions. The study first...
Arterial tortuosity describes variation via bending of the arterial wall and has been noted in several arteries throughout body. Tortuous blood vessels can cause nerve compression, as well present difficulties to surgeons radiologists. Here we an unusual case multi-vessel discovered 78-year-old Hispanic male cadaver, independent systemic pathology. The left ulnar right tibial were dissected, using calibrated digital callipers, their external internal diameters measured both at origin site...