A5047145747- Multiple Sclerosis Research Studies
- Immunotherapy and Immune Responses
- MicroRNA in disease regulation
- Economic and Fiscal Studies
- Accounting Theory and Financial Reporting
- Neurogenesis and neuroplasticity mechanisms
- Immune Cell Function and Interaction
- Heat shock proteins research
- Cell death mechanisms and regulation
- Developmental Biology and Gene Regulation
- Systemic Lupus Erythematosus Research
- T-cell and B-cell Immunology
- RNA Interference and Gene Delivery
- Mesenchymal stem cell research
- SARS-CoV-2 and COVID-19 Research
- Invertebrate Immune Response Mechanisms
- Studies on Chitinases and Chitosanases
- Nanoparticle-Based Drug Delivery
- Management and Organizational Practices
- Trace Elements in Health
- Peripheral Neuropathies and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune cells in cancer
- Taxation and Legal Issues
- Axon Guidance and Neuronal Signaling
Medical University of Lodz
2008-2024
University of Cologne
2018-2024
University of Łódź
2013-2015
The University of Texas Health Science Center at Houston
2009
Albert Einstein College of Medicine
2007
Montreal Neurological Institute and Hospital
1998-2000
McGill University
1998-2000
Abstract Knockout of caspase-8, a cysteine protease that participates in the signaling for cell death by receptors TNF/nerve growth factor family, is lethal to mice utero. To explore tissue-specific roles this enzyme, we established its conditional knockout using Cre/loxP recombination system. Consistent with role induction, deletion caspase-8 hepatocytes protected them from Fas-induced caspase activation and death. However, application approach investigate cause embryos revealed enzyme also...
Inositol has 8 stereoisomers, four of which are physiologically active. <i>myo</i>-Inositol is the most abundant isomer in brain and more recently shown that <i>epi</i>- and<i>scyllo</i>-inositol also present. complexes with Aβ42 <i>in vitro</i> to form a small stable micelle. The ability inositol stereoisomers interact stabilize Aβ was addressed. Circular dichroism spectroscopy demonstrated <i>scyllo</i>- but not <i>chiro</i>-inositol were able induce structural transition from random...
Multiple sclerosis (MS) is considered to be an autoimmune disease that directed either at myelin or its cell of origin, the oligodendrocytes (OL). The inflammatory lesions in central nervous system contain multiple Ag-restricted and nonrestricted populations with potential mediate tissue injury. Previous studies indicate it possible generate MHC class I-restricted peptide-specific cytotoxic CD8 T cells, human adult OLs express I molecules vitro. purpose this study was demonstrate basic...
Tumour necrosis factor (TNF)-induced death of oligodendrocytes, the cell type targeted in multiple sclerosis, is mediated by TNF receptor p55 (TNFR-p55). The ligation TNFR-p55 induces several signal transduction pathways; however, precise mechanism involved human oligodendrocyte (hOL) unknown. We defined that TNF-induced hOLs non-caspase dependent, as evidenced lack generation caspases 8, 1 and 3 active subunits; cleavage fluorogenic substrates; hOL inhibition general caspase inhibitor,...
Abstract Objective: Antigen‐specific therapy targeting selective inhibition of autoreactive responses holds promise for controlling multiple sclerosis (MS) without disturbing homeostasis the whole immune system. Key autoantigens in MS include myelin proteins, such as basic protein (MBP), proteolipid (PLP), and oligodendrocyte glycoprotein (MOG). In this study, we examined effect transdermal with peptides on skin, lymph nodes, peripheral blood cells patients. Methods: a 1‐year...
Abstract Among its varied functions, Notch signaling is involved in peripheral T cells responses. The activation and polarization of CD4+ toward a Th1 lineage are essential steps the pathogenesis multiple sclerosis animal model, experimental autoimmune encephalomyelitis. Inhibition all four receptors with γ-secretase inhibitor was shown to block Th1-type attenuate symptoms In this study, we have examined role individual proliferation, cytokine production, encephalitogenic potential...
Integrating imaging and therapeutic capabilities into a single entity can offer enhanced diagnostic accuracy treatment efficacy in clinically effective formulations. Due to the diversity of chemical structures and/or limited solubility inhibitors or fluorophores, it is essential employ robust delivery carrier that facilitate drug absorption distribution during its circulation blood. This study explores potential hollow gadolinium oxide (Gd2O3) nanocarriers applications. The citric acid...
Tumour necrosis factor (TNF) induces death of oligodendrocytes, the putative cell target in multiple sclerosis. We defined that intracellular transduction pathway involved TNF-induced human adult oligodendrocytes (hOLs) is dependent on c-jun NH(2)-terminal kinase (JNK) activation, but not other mitogen-activated protein (MAPK), p38. JNK measured by phosphorylation and induction phosphorylated form JNK, was enhanced, prolonged correlated with hOLs exposed to TNF. Comparative autoradiographic...
<b>Background: </b> CNS axons display a poor regenerative response to injury. In multiple sclerosis (MS), failure of damaged regenerate may be major factor underlying non-reversible neurologic dysfunction. Nogo is development-related molecule inhibiting axonal regeneration and component myelin. <b>Methods: CSF from 114 patients with remitting relapsing MS (RR-MS) secondary progressive (SP-MS) 153 controls, as well tissue 3 2 were used for this study. <b>Results: We found soluble 20 kDa...
The molecular mechanisms that regulate the immune function of bone marrow-derived mesenchymal stem cells (BMSCs) are not known. We have shown previously freshly isolated BMSCs when induced to express neuronal cell markers lose immunoregulatory transferred into mice sensitized develop experimental autoimmune encephalomyelitis. Recently, microRNAs (miRs) been be involved in regulation several responses both innate and acquired immunity. now show among differentially expressed miRs, miR-146a...
MicroRNAs (miRNAs) associate with Argonaute (Ago), GW182, and FXR1 proteins to form RNA-induced silencing complexes (RISCs). RISCs represent a critical checkpoint in the regulation bioavailability of miRNAs. Recent studies have revealed dysregulation miRNAs multiple sclerosis (MS) its animal model, experimental autoimmune encephalomyelitis (EAE); however, function EAE MS is largely unknown. Here, we examined expression Ago, CNS tissue, oligodendrocytes (OLs), brain-infiltrating T...
YKL-40 (CHI3L1) is a matrix glycoprotein stored in human neutrophil-specific granules and released upon activation. While it implicated inflammation, cancer progression, cell differentiation, its exact physiological role remains unclear. This study investigated the intracellular expression secretion of by untreated DMSO-treated HL-60 cells association with surface CD11b CD66b throughout differentiation process (up to 120 h). Secreted protein mRNA levels YKL-40, CD66b, were measured ELISA...
Abstract Tumor necrosis‐related apoptosis‐inducing ligand (TRAIL) induces apoptosis of oligodendrocytes, target cells immune attack in multiple sclerosis (MS). TRAIL‐induced human oligodendrocyte (hOL) death depends on TRAIL ligation with its receptor 1 (TRAIL‐R1). However, the intracellular signaling initiated TRAIL‐R1 hOLs is unknown. We defined that transduction involved associated strong activation c‐jun NH 2 ‐terminal kinase (JNK) and a dominant negative mutant MKK4/SEK1, MAP upstream...
(1) Background: The present study aims to report the side effects of vaccination against coronavirus disease 2019 (COVID-19) among patients with multiple sclerosis (MS) who were being treated disease-modifying therapies (DMTs) in Poland. (2) Methods: included 2261 MS DMTs, and vaccinated COVID-19 16 Polish centers. data collected demographic information, specific characteristics, current type vaccine, after vaccination, time side-effect symptom onset resolution, applied treatment, relapse...
Abstract Heat shock proteins (Hsp) are markedly up-regulated at sites of inflammation during autoimmune diseases like experimental encephalomyelitis (EAE). In this study, we show that Hsp70-peptide complexes (pc) isolated from brains mice with EAE prevented the development clinically and pathologically when administered before proteolipid protein 139–151 (PLP139–151) immunization. contrast, pure Hsp70 or Hsp70-pc derived healthy other inflamed tissue did not modulate expression EAE. animals...
Iron oxide nanoparticles (NPs) are extensively used in various biomedical applications due to their suitability as carriers of diagnostic and therapeutic agents. Herein, we report on the evaluation concentration-dependent toxicity studies iron (magnetite) NPs zebrafish (Danio rerio). To follow update animal model, particles were functionalized by conjugating a fluorescent dye (Congo red) that serves an efficient probe track uptake accumulations from early life stages zebrafish. As vivo model...
Abstract Inflammation leads to induction of tissue stress conditions that might contribute the generation mechanisms limiting ongoing immune responses. We have shown previously peptides derived from brain mice with experimental autoimmune encephalomyelitis (EAE) complexed chaperone heat shock protein 70 (Hsp70-pc) induce an NK-cell-dependent tolerance for subsequent EAE sensitization. now present data showed MHC class I-related glycoprotein H60 determines Hsp70-pc-induced inhibition....
Heat shock proteins (Hsps) interact with the immune system and have been shown to contribute immunoregulation. As efficient chaperones, Hsps bind many peptides these complexes yet-to-be-clarified functions. We that Hsp70 is complexed within mouse CNS peptide CLAFHDISPQAPTHFLVIPK derived from histidine triad nucleotide-binding protein-1 (HINT1₃₈₋₅₇/Hsp70). Only this complex, in contrast other Hsp70, was able prevent experimental autoimmune encephalomyelitis (EAE) by induction of...
Since vaccination against COVID-19 is available for over a year and the population of immunized individuals with autoimmune disorders higher than several months before, an evaluation safety registered adverse events can be made. We conducted large study side effects following vaccine among patients multiple (MS) sclerosis treated disease-modifying therapies (DMTs) analyzed factors predisposing particular events.We gathered data MS DMTs from 19 Polish Centers, who reported at least one event...
Hollow mesoporous silica capsules (HMSC) are potential drug transport vehicles due to their biocompatibility, high loading capacity and sufficient stability in biological milieu. Herein, we report the synthesis of ellipsoid-shaped HMSC (aspect ratio ∼2) performed using hematite particles as solid templates that were coated with a conformal shell through cross-condensation reactions. For obtaining hollow capsules, iron oxide core was removed by acidic leaching. Gas sorption studies on...