A5101887003- Fungal and yeast genetics research
- Microbial Metabolic Engineering and Bioproduction
- Advanced biosensing and bioanalysis techniques
- Chromium effects and bioremediation
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Plant nutrient uptake and metabolism
- CRISPR and Genetic Engineering
- DNA and Nucleic Acid Chemistry
- Biofuel production and bioconversion
- Algal biology and biofuel production
- Genomics and Phylogenetic Studies
- Fibroblast Growth Factor Research
- RNA regulation and disease
- Polysaccharides and Plant Cell Walls
- Molecular Sensors and Ion Detection
- Alzheimer's disease research and treatments
- Corneal Surgery and Treatments
- Mitochondrial Function and Pathology
- Lipid Membrane Structure and Behavior
- Microbial Fuel Cells and Bioremediation
- Monoclonal and Polyclonal Antibodies Research
- Plant-Microbe Interactions and Immunity
- Surfactants and Colloidal Systems
- Genetics, Aging, and Longevity in Model Organisms
University of Florida
2020-2024
Foundation for Applied Molecular Evolution
2023-2024
Firebird Biomolecular Sciences (United States)
2024
Interface (United States)
2020-2024
Binzhou University
2019-2023
University of Florida Health
2022
Chinese Academy of Sciences
2014-2022
University of Chinese Academy of Sciences
2017-2022
Cancer Hospital of Chinese Academy of Medical Sciences
2022
Zhejiang Cancer Hospital
2022
The glucose dual-affinity transport system (low- and high-affinity) is a conserved strategy used by microorganisms to cope with natural fluctuations in nutrient availability the environment. glucose-sensing uptake processes are believed be tightly associated cellulase expression regulation cellulolytic fungi. However, both identities functions of major molecular components this evolutionarily filamentous fungi remain elusive. Here, we systematically identified characterized model fungus...
Lipid-oligonucleotide conjugates (LONs) are powerful molecular-engineering materials for various applications ranging from biosensors to biomedicine. Their unique amphiphilic structures enable the self-assembly and conveyance of information with high fidelity. In particular, LONs present remarkable potential in measuring cellular mechanical forces monitoring cell behaviors. also essential sensing tools intracellular imaging have been employed developing cell-surface-anchored DNA...
Aptamers developed using in vitro Systematic Evolution of Ligands by Exponential Enrichment (SELEX) technology are single-stranded nucleic acids 10–100 nucleotides length. Their targets, often with specificity and high affinity, range from ions small molecules to proteins other biological as well larger systems, including cells, tissues, animals. rival conventional antibodies improved performance, due aptamers’ unique biophysical biochemical properties, size, synthetic accessibility, facile...
pH-responsiveness has been widely pursued in dynamic DNA nanotechnology, owing to its potential biosensing, controlled release, and nanomachinery. pH-triggering systems mostly depend on specific designs of sequences. However, sequence-independent regulation could provide a more general tool achieve pH-responsive assembly, which yet be developed. Herein, we propose mechanism for assembly by utilizing ethylenediamine (EN) as reversibly chargeable (via protonation) molecule overcome...
CRISPR-Staphylococcus aureus Cas9 (CRISPR-SaCas9) has been harnessed as an effective in vivo genome-editing tool to manipulate genomes. However, off-target effects remain a major bottleneck that precludes safe and reliable applications genome editing. Here, we characterize the of wild-type (WT) SaCas9 at single-nucleotide (single-nt) resolution describe directional screening system identify novel variants with desired properties human cells. Using this system, identified enhanced-fidelity...
Pathological hyperphosphorylation and aggregation of microtubule-associated Tau protein contribute to Alzheimer's Disease (AD) other related tauopathies. Currently, no cure exists for Disease. Aptamers offer significant potential as next-generation therapeutics in biotechnology the treatment neurological disorders. Traditional aptamer selection methods focus on binding affinity rather than interference with pathological Tau. In this study, we developed a new strategy enrich DNA aptamers that...
Neurospora crassa recently has become a novel system to investigate cellulase induction. Here, we discovered membrane protein, cellodextrin transporter-like protein 1 (CLP1; NCU05853), putative that is critical component of the induction pathway in N. crassa. Although CLP1 cannot transport cellodextrin, suppression by this was on both cellobiose and Avicel. The co-disruption transporters cdt2 clp1 strain Δ3βG formed CPL7. With cellobiose, production enhanced 6.9-fold CPL7 compared with Δ3βG....
CDT-1 and CDT-2 are two cellodextrin transporters discovered in the filamentous fungus Neurospora crassa. Previous studies focused on characterizing role of these only a few conditions, including cellulose degradation, function is not yet completely understood. In this study, we show that deletion cdt-2, but cdt-1, results growth defects Avicel also xylan. cdt-2 can be highly induced by xylan, mutant has xylodextrin consumption defect. Transcriptomic analysis strain xylan showed major...
Limited uptake is one of the bottlenecks for l-arabinose fermentation from lignocellulosic hydrolysates in engineered Saccharomyces cerevisiae. This study characterized two novel transporters, LAT-1 Neurospora crassa and MtLAT-1 Myceliophthora thermophila. Although proteins share high identity (about 83%), they display different substrate specificities. Sugar transport assays using S. cerevisiae strain EBY.VW4000 indicated that accepts a broad spectrum. In contrast, appeared much more...
Abstract With just four building blocks, low sequence information density, few functional groups, poor control over folding, and difficulties in forming compact folds, natural DNA RNA have been disappointing platforms from which to evolve receptors, ligands, catalysts. Accordingly, synthetic biology has created “artificially expanded genetic systems” (AEGIS) add nucleotides, functionality, density. the expected improvements seen AegisBodies AegisZymes, task for biologists shifts developing...
Crop residue is an abundant, low-cost plant biomass material available worldwide for use in the microbial production of enzymes, biofuels, and valuable chemicals. However, diverse chemical composition complex structure crop residues are more challenging efficient degradation by microbes than homogeneous polysaccharides. In this study, transcriptional responses Neurospora crassa to various straws were analyzed using RNA-Seq, novel beneficial factors biomass-induced enzyme...
Genome editing using RNA‐guided nucleases in their ribonucleoprotein (RNP) form represents a promising strategy for gene modification and therapy because they are free of exogenous DNA integration have reduced toxicity vivo ex vivo. However, genome by Cas9 nuclease from Staphylococcus aureus (SaCas9) has not been reported its RNP form, which recognizes longer protospacer adjacent motif (PAM), 5′‐NNGRRT‐3′, compared with Streptococcus pyogenes (SpCas9) 5′‐NGG‐3′ PAM. Here, SaCas9‐RNP‐mediated...
Clustered regularly interspaced short palindromic repeat-Cas12a has been harnessed to manipulate the human genome; however, low cleavage efficiency and stringent protospacer adjacent motif hinder use of Cas12a-based therapy applications. Here, we have described a directional evolving screening system in cells identify novel FnCas12a variants with high activity. By using this system, identified IV-79 (enhanced activity FnCas12a, eaFnCas12a), which possessed higher DNA than WT FnCas12a....
CRISPR/Cas9 nucleases are widely used for genome editing but can induce unwanted off-target mutations. High-fidelity Cas9 variants have been identified; however, they often reduced activity, constraining their utility, which presents a major challenge use in research applications and therapeutics. Here we developed tRNAGln-processing system to restore the activity of multiple high-fidelity human cells, including SpCas9-HF1, eSpCas9, xCas9. Specifically, acting on previous observations that...
A bispecific circular aptamer was utilized to tether a built-in His tag specific cells enhance the delivery of functional proteins.
Abstract pH‐responsiveness has been widely pursued in dynamic DNA nanotechnology, owing to its potential biosensing, controlled release, and nanomachinery. pH‐triggering systems mostly depend on specific designs of sequences. However, sequence‐independent regulation could provide a more general tool achieve pH‐responsive assembly, which yet be developed. Herein, we propose mechanism for assembly by utilizing ethylenediamine (EN) as reversibly chargeable (via protonation) molecule overcome...
Gene gain and loss frequently occurs in the cyanobacterium Prochlorococcus, a phototroph that numerically dominates tropical subtropical open oceans. However, little is known about stabilization of its core genome, which contains approximately 1250 genes, context genome streamlining. Using Prochlorococcus MED4 as model organism, we investigated constraints on using transcriptome profiling. RNA-Seq technique was used to obtain map MED4, including operons, untranslated regions, non-coding...
<title>Abstract</title> Adding nucleotide pairs to a DNA alphabet not only increases the linear information density of molecules, but also may increase diversity folds available molecular system. Reported here is an example this. A single additional nucleotide, with 5-nitro-6-aminopyridone ("Z") heterocycle, selected from artificially expanded genetic system (AEGIS), was incorporated at twelve sites into strand 23 nucleotides in length. With fluor its 5'-end and quencher 3'-end, this...
Abstract Stimuli responsivity has been extensively pursued in dynamic DNA nanotechnology, due to its incredible application potentials. Among diverse systems, redox‐responsive assembly holds great promise for broad applications, especially considering that redox processes widely exist various physiological environments. However, only a few studies have reported on redox‐sensitive assembly. Albeit ingenious, most of these are either dependent the sequence or involve chemical modification....
The lignocellulolytic filamentous fungus Neurospora crassa is able to assimilate various mono- and oligo-saccharides. However, more than half of predicted sugar transporters in the genome are still waiting for functional elucidation. In this study, system analysis substrate spectra N. was performed at genome-wide level. NCU01868 NCU08152 have capability uptaking hexose, which named as NcHXT-1 NcHXT-2 respectively. Their transport activities glucose were further confirmed by fluorescence...
Abstract Background Fibroblast growth factor 19 (FGF19) takes part in maintaining the balance of glycolipids and may be involved complications type 1 diabetes(T1D) children. This study aimed at evaluating relationship among levels serum FGF19 vascular endothelial factor(VEGF)and soluble klotho protein(sklotho) diabetic Methods In a cross-section single center samples were obtained from 96 subjects: 66 T1D 30 healthy children.Serum VEGF sklotho concentrations measured by ELISA. And diabetes...
Abstract Pathological hyperphosphorylation and aggregation of microtubule‐associated Tau protein contribute to Alzheimer's Disease (AD) other related tauopathies. Currently, no cure exists for Disease. Aptamers offer significant potential as next‐generation therapeutics in biotechnology the treatment neurological disorders. Traditional aptamer selection methods focus on binding affinity rather than interference with pathological Tau. In this study, we developed a new strategy enrich DNA...
To investigate the relationship between semaphorin 7a expression and cell proliferation migration in pterygium fibroblasts.Twenty-six patients with surgically diagnosed were enrolled, including 15 cases of primary 11 recurrent pterygium. In addition, 12 normal conjunctival tissue collected. The tissue, was detected by real-time polymerase chain reaction. Recurrent fibroblasts isolated cultured, silenced small interfering RNA (siRNA) interference technique. Furthermore, effects si-semaphorin...