Peru is currently one of the world’s leading culinary destinations, whose world-renowned cuisine uses vegetables, mainly lettuce, as frequent ingredients. Vegetable consumption promoted worldwide a part healthy diet. However, more frequently have been implicated vehicle infection for several foodborne parasites. This study aimed to determine presence potentially pathogenic parasites in lettuce marketed Central Andes Peru. A total 75 samples were collected from two largest wholesale markets...

Background: Recently, it was proposed the name of Sarcocystis masoni n. sp. for that causes microcyst in skeletal muscle South American camelids. However, there are no ultrastructural reports microcysts cardiac alpacas. This study features from naturally infected Methods: Thirty alpacas (age range: three to five years) province Junin, Peruvian Central Andes, were included this January 2015. Cardiac samples evaluated by histology and transmission electron microscopy. Results: Bradyzoites...

Se evaluó la presencia de enteropatógenos en 60 crías alpacas 1 a 2.5 meses edad con cuadros mortales diarrea las regiones Pasco y Junín, Perú. tomaron muestras heces para determinar frecuencia Escherichia coli Salmonella sp mediante microbiología convencional, parásitos gastrointestinales por técnicas sedimentación flotación, Criptosporidium técnica Ziehl Neelsen Modificado. Además, se evidenció antígenos virales ayuda un kit comercial inmunocromatografía. identificó E. (80%), coronavirus...

El objetivo del presente trabajo fue determinar si los puestos de venta carne pollo son una fuente contaminación con Escherichia coli shigatoxigénica (STEC) en mercados abastos. Se tomaron hisopados la superficie manos, tablas picar y mesas expendio 50 el distrito San Juan Miraflores, Lima, Perú (n=150 muestras). realizó aislamiento microbiológico estándar e identificación molecular genes stx1, stx2 eaeA mediante PCR. 42% (63/150) 25.3% (38/150) las muestras fueron positivas a E. STEC,...

Procedures were evaluated for the rapid and efficient purification of oocysts esporocysts Sarcocystis aucheniae (Sa) from small intestine experimentally infected dogs with 400 Sa macrocysts obtained meat naturally alpacas. The mucosa was removed. Samples washed by centrifugation PBS, homogenized centrifuged. Large quantities attached to intestinal microvilli obtained. In first procedure, an ezimatic treatment 5% tripsin used resulting in 50% oocyst separation. second procedure samples...

El objetivo del estudio fue determinar la presencia de Listeria monocytogenes en muestras canales porcinas Lima, Perú. Se muestrearon 88 superficies procedentes un centro beneficio. Las se sometieron a pre-enriquecimiento medio BLEB y enriquecimiento microbiológico caldo PALCAM, el patógeno identificó con técnica molecular detección rápida hibridación in situ. 13.9 ± 6.1% las resultaron positivas L. monocytogenes, posiblemente debido deficiencias aplicación control buenas prácticas...

We report cystic echinococcosis (CE) prevalence in Huancayo Province, Peru, and the associated economic effect of bovine organ condemnation. CE during 16-month study period was 42.8% caused $14,595 losses. threatens food security region by reducing farmers' income viscera supply markets.

The aim of this study was to determine the presence Campylobacter spp in meat and cecum broilers sold three illegal abattoirs Lima, Peru. For purpose, 90 were selected, collecting 60 samples body carcasses by rinse method 30 cecum. cultured mCCDA agar at 42 °C for 48 h under microaerophilic atmosphere, biochemical tests used identify thermotolerant species. results showed that 16.7% positive, where 60% C. jejuni 40% coli. In samples, 26.7% positive spp, 72.7% 27.2%

Physical and chemical methods of home use techniques for sanitation detoxification alpaca meat infected with macrocysts Sarcocystis aucheniae were evaluated. The physical treatments boiling baking at 80 °C 5 minutes freezing 10, 15 20 days; the salting 30 days, marinated 24 hours. Dogs fed bouts treated containing 180-200 presence oocysts analyzed in faeces 18 days. Besides, lisates injected to rabbits evaluate detoxifying effect on protein content macrocysts. Only proved be effective...

Abstract Boning of dry‐cured hams involves additional handling which increases the risk contamination with Listeria monocytogenes . This work studies ability E‐beam to eliminate this pathogen from boned ham (BDH). The destruction kinetics four L. strains (S2, S4‐2, S12‐1, and S7‐2) innocua NCTC 11288 as a surrogate were determined at doses up 3 kGy. S4‐2 most radioresistant ( D ‐value = 0.55 kGy). penetration was studied in blocks extracted BDH irradiated 2–6 bilateral treatment 2 kGy allows...

Objetivo: Identificar las condiciones de manejo e infraestructura en la crianza no tecnificada cerdos una zona peri-urbanas Lima. Metodología: Se encuesto a 51 productores. tomaron datos como información demográfica y características del sistema productivo. Resultados Conclusiones: La edad promedio los productores fue 51.3 años (rangos 19 74 años) el tiempo que dedicaban actividad 10.8 01 45 años). registró propiedad un total 1401 animales (290 marranas, 34 verracos, 622 gorrinos 455...

Alpacas in the pre-weaning stage often present neonatal diarrhoea, usually of infectious type, causing high mortality. This study determined physiological parameters and their changes animals with diarrhoea. In total, 79 neonate alpacas less than 60 days age from Pasco Junín regions, Peru, were used. Of them, had clinical diarrhoea lasting 1 to 3 days, 19 healthy (controls). Heart respiratory rate, peripheral capillary oxygen saturation, body temperature, refill time, mucous color...

Hot and cold smoked, dry wet cured, wet-cured dry-cure smoked techniques for sanitation detoxification of alpaca meat naturally infected with macrocysts Sarcocystis aucheniae were evaluated. Eighteen puppies 4-6 months age fed the treated (100 per puppy). Lysates from meats prepared inoculated into 18 rabbits μ/kg body weight, subcutaneously). Furthermore, lysates boiled (100º C 10 min) 8 rabbits. Only dogs positive control group cured eliminated sporocysts after 15 days ingestion. None...

A total of 96 samples (3 scalding water, 3 final washing 30 non-eviscerated, eviscerated and cecal content) from three small-scale broiler slaughterhouses were evaluated. Bacteriological test was performed with mCCDA medium positive confirmed by PCR assays using 16S rDNA, hipO asp primers to Campylobacter spp., jejuni coli respectively. showed the presence spp. in 60 samples. However, according assays, only 19 as positive. Non-eviscerated carcasses had fewer numbers for (63 50%,...

The aim of this study was to identify and assess the presence glucose transporters SGLT1 GLUT2 incretin GLP-1 in small intestine guinea pig (Cavia porcellus). Five animals 90 days old were used. Samples from duodenum, jejunum ileum collected after slaughtering carry out immunohistochemistry assays. Expression SGLT1, observed all three portions intestine, where showed highest expression, with a strong staining intensity apical medium section crypt-villus axis. throughout axis jejunum....

The purpose of this study was to evaluate the degree faecal contamination in three vegetables major raw consumption that are sold four large wholesale markets Lima, Peru. A total 180 samples, 15 lettuce (Lactuca sativa), cabbage (Brassica oleracea) and spinach (Spinacea from La Parada, Ramón Castilla, Ceres Caquetá were collected within a year. Samples processed using Most Probable Number method for coliform E. coli Type I (Typical) detection count, plus Absence/Presence test Salmonella. In...

The aim of this study was to determine the presence Salmonella spp in broilers from 17 illegal abattoirs Lima, Peru. Samples were collected body surface carcasses (using rinsing method) and cloacal swabs 170 broilers. isolation identification done through conventional laboratory methods. Results showed 23.5% samples 32.4% positive spp, without differences between where evisceration or not carried out. degree concordance results for both sampling methods significant (k = 0.074, k 0.146),...