A5010956781- Antibiotic Resistance in Bacteria
- Gut microbiota and health
- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotic Use and Resistance
- Viral gastroenteritis research and epidemiology
- Parasitic Infections and Diagnostics
- Travel-related health issues
- Phenothiazines and Benzothiazines Synthesis and Activities
- Microbial Community Ecology and Physiology
- Ethics in Clinical Research
- Antimicrobial agents and applications
- Cancer Research and Treatments
- Antibiotics Pharmacokinetics and Efficacy
- Click Chemistry and Applications
- Advanced biosensing and bioanalysis techniques
- Nanoplatforms for cancer theranostics
- Biosensors and Analytical Detection
- Health and Medical Research Impacts
Washington University in St. Louis
2023-2025
Edison (Italy)
2025
The history of clinical resistance to tetracycline antibiotics is characterized by cycles whereby the deployment a new generation drug molecules quickly followed discovery mechanism resistance. This suggests mechanism-specific selection each generation; however, evolutionary dynamics this remain unclear. Here, we evaluate 24 recombinant Escherichia coli strains expressing genes from (efflux pumps, ribosomal protection proteins, and enzymatic inactivation) in context generation. We employ...
There is a paucity of research on detailed antibiotic resistome and microbiome diversity Indian hospital wastewater. This study reports the predominance clinically concerning ARGs such as beta-lactamases bla NDM OXA colistin resistance gene mcr their association with in six different wastewaters both urban rural origin.
Tetracyclines (TCs) are an important class of antibiotics threatened by enzymatic inactivation. These tetracycline-inactivating enzymes, also known as tetracycline destructases (TDases), a subfamily A flavin monooxygenases (FMOs) that catalyze hydroxyl group transfer and oxygen insertion (Baeyer-Villiger type) reactions on TC substrate scaffolds. Semisynthetic modification TCs (e.g., tigecycline, omadacycline, eravacycline, sarecycline) has proven effective in evading certain resistance...
Abstract International travel contributes to the global spread of antimicrobial resistance. Travelers’ diarrhea exacerbates risk acquiring multidrug-resistant organisms and can lead persistent gastrointestinal disturbance post-travel. However, little is known about impact on travelers’ gut microbiomes, dynamics these changes throughout travel. Here, we assembled a cohort 159 international students visiting Andean city Cusco, Peru applied next-generation sequencing techniques 718...
Abstract Tetracycline destructases (TDases) are flavin monooxygenases which can confer resistance to all generations of tetracycline antibiotics. The recent increase in the number and diversity reported TDase sequences enables a deep investigation sequence-structure-function landscape. Here, we evaluate sequence determinants function through two complementary approaches: (1) constructing profile hidden Markov models predict new TDases, (2) using multiple alignments identify conserved...
Tetracyclines (TCs) are an important class of antibiotics threatened by emerging new resistance mechanism─enzymatic inactivation. These TC-inactivating enzymes, also known as tetracycline destructases (TDases), inactivate all TC antibiotics, including drugs last resort. Combination therapies consisting a TDase inhibitor and antibiotic represent attractive strategy for overcoming this type resistance. Here, we report the structure-based design, synthesis, evaluation bifunctional inhibitors...
Abstract Inactivation of tetracycline antibiotics by destructases (TDases) remains a clinical and agricultural threat. TDases can be classified as type 1 Tet(X)-like 2 soil-derived TDases. Type are widely identified in pathogens. A combination therapy TDase inhibitor is much needed to rescue the efficacy tetracyclines. Anhydrotetracycline pan-TDase that inhibits both Here, we present structural, biochemical, phenotypic evidence anhydrotetracycline binds substrate-like orientation...
ABSTRACT International travelers are frequently afflicted by acute infectious diarrhea, commonly referred to as travelers’ diarrhea (TD). Antibiotics often prescribed treatment or prophylaxis for TD; however, little is known about the impacts of these regimens on gut microbiomes and carriage antibiotic resistance genes (ARGs). Here, we analyzed two cohorts totaling 153 US UK servicemembers deployed Honduras Kenya. These subjects either experienced TD during deployment received a single dose...