To evaluate the biomechanical effect of combined riboflavin-ultraviolet A (UVA) treatment on porcine and human corneas.Department Ophthalmology, Technical University Dresden, Germany.Corneal strips from 5 enucleated eyes 20 cadaver corneas were treated with photosensitizer riboflavin irradiated 2 double UVA diodes (370 nm, irradiance = 3 mW/cm2) for 30 minutes. After cross-linking, static stress-strain measurements untreated performed using a microcomputer-controlled biomaterial tester...

Collagen-crosslinking using combined riboflavin/ UVA treatment has been developed by us as a new for keratoconus stiffening the collagenous matrix. Recently, we have started to use same method of corneal ulcers. The aim present study was evaluate influence crosslinking on resistance cornea against enzymatic degradation.60 enucleated porcine eyes were treated with photosensitizer riboflavin and UVA-irradiation (370 nm; irradiance 1, 2 or 3 mW/cm2) 30 minutes compared 20 untreated control...

Collagen crosslinking of the cornea has been developed recently as a quasiconservative treatment keratoconus. Biomechanical in vitro measurements have demonstrated significant increase biomechanical stiffness crosslinked cornea. The aim present study was to evaluate effect this new procedure on collagen fiber diameter rabbit cornea.The corneas right eyes 10 New Zealand White albino rabbits were by application photosensitizer riboflavin and exposure UVA light (370 nm, 3 mW/cm2) for 30...

To test the biomechanical efficiency of corneal crosslinking with riboflavin without epithelial debridement (C3-R).Moscow Helmholtz Research Institute Eye Diseases, Moscow, Russia.The left eyes rabbits were crosslinked using standard including removal (Group 1), benzalkonium chloride-containing proxymetacaine eyedrops 2), or preservative-free oxybuprocaine 3). All received solution and irradiated an ultraviolet-A double diode for 30 minutes (irradiance 3 mW/cm(2)). The animals killed 1 day...

Combined riboflavin/UVA treatment inducing collagen cross-links in the cornea has been shown to increase biomechanical rigidity of and used successfully progressive keratoconus. The current study was undertaken investigate possible cytotoxic effect combined on corneal keratocytes vivo.Thirty-four New Zealand white rabbits were treated with 0.1% riboflavin solution surface UVA irradiances ranging from 0.75 4 mW/cm2 (1.35- 7.2 J/cm2) for 30 minutes. animals euthanized either (n = 6) or 24 28)...

To evaluate the possible cytotoxic effect of combined riboflavin-ultraviolet-A (UVA) treatment on corneal endothelium.Department Ophthalmology, Technical University Dresden, Germany.The right eyes 34 New Zealand White rabbits were treated with riboflavin and various endothelial UVA doses ranging from 0.16 to 0.9 J/cm2 (0.09 0.5 mW/cm2, 370 nm) postoperative enucleation times 4 hours 24 hours. The cells evaluated in histological sections. terminal deoxynulceotidyl transferase...

Recently, we have developed collagen crosslinking induced by combined riboflavin/UVA treatment, thus increasing the biomechanical rigidity of cornea to treat progressive keratoconus. The present safety study was performed evaluate possible cytotoxic effects treatment on corneal endothelium in vitro. Endothelial cell cultures from porcine corneas were treated with 500 microM riboflavin solution, exposed various endothelial UVA irradiances (370 nm) ranging 0.1 1.6 mW/cm2 for 30 min and...

To develop methods of collagen crosslinking the sclera to increase its biomechanical strength for treatment progressive myopia.Department Ophthalmology, Technical University Dresden, Germany.Sagitally oriented scleral strips 4.0 mm x 8.0 were prepared from 5 human postmortem eyes and 50 porcine cadaver treated with various including physical by combined riboflavin-ultraviolet A (UVA) or rose bengal/white-light irradiation chemical incubation glucose, ribose, glyceraldehyde, glutaraldehyde...

To examine the influence of a new crosslinking treatment on corneal swelling properties that correlate with degree crosslinking.Department Ophthalmology, Vivantes-Klinikum Neukölln, Berlin, Germany.Twenty freshly enucleated porcine eyes were crosslinked by applying photosensitizer riboflavin and ultraviolet-A (UVA) light (370 nm, 3 mW/cm2) for 30 minutes. After treated incubated 24 hours in moist chamber, 15 examined biomicroscopy optical coherence tomography (OCT); 5 microscopy. Five...

Abstract. Purpose: Photodynamic riboflavin/ultraviolet‐A (UVA)‐induced collagen cross‐linking, which increases the biomechanical stiffness of human cornea by about 300%, has been introduced recently as a possible treatment for progressive keratoconus. The present study was undertaken to evaluate longterm effects this new cross‐linking necessary prerequisite its clinical success. Methods: corneas left eyes nine male rabbits were cross‐linked. contralateral served controls. After removal...

To evaluate the role of preocular riboflavin film in ultraviolet-A (UVA) absorption corneal collagen crosslinking (CXL).Eye Laser Institute, Department Ophthalmology, Martin-Luther-University, Halle, Germany.The UVA light was measured human donor and porcine postmortem corneas with without using 3 solutions: standard dextran-riboflavin, methylcellulose-riboflavin, hypoosmolar riboflavin-sodium chloride dextran. The breakup time solutions their absorbance were also determined.After 30-minute...

Collagen cross-linking using combined riboflavin/UVA treatment has been shown to increase the biomechanical rigidity of cornea and used successfully for progressive keratoconus. From morphological biochemical investigations, a different degree anterior posterior stroma by is suggested. The present study was undertaken better evaluate this effect testing thermomechanical behavior.Ten 10 x 5 mm corneal strips from porcine cadaver eyes enucleated within h post mortem were cross-linked...

This study was undertaken to investigate the wound healing process of first 6 weeks after photodynamic cross-linking treatment in rabbit cornea, using photosensitizer riboflavin and UVA.After removal central epithelium, right corneas 8 Chinchilla rabbits were cross-linked with a photosensitizing 0.1% solution UVA light (370 nm; irradiance, 3 mW/cm(2); dose, 5.4 J/cm(2)) for 30 minutes. Two animals euthanized days, 7 4 weeks, postoperatively. The enucleated eyes evaluated 4-microm microscopic...

Scleral crosslinking by the photosensitizer riboflavin and ultraviolet A (UVA) has been shown to increase significantly scleral biomechanical rigidity might therefore become a possible sclera-based treatment modality for progressive myopia. In present study, long-term effect of new method on properties was investigated in rabbit sclera.A 10 x mm sector equatorial sclera nine Chinchilla eyes treated vivo using UVA double diode 370 nm with surface irradiance 3 mW/cm(2) application 0.1%...

Scleral biomechanical weakness and thinning is known to be one of the main factors in pathogenesis progressive myopia. We tried strengthen rabbit sclera by cross-linking scleral collagen using ultraviolet A (UVA) photosensitizer riboflavin.Circumscribed 10 x10 mm sectors posterior--equatorial six chinchilla eyes were treated vivo a UVA double diode with 4.2 mW/cm(2) at 370 nm applying 0.1% riboflavin-5-phosphate drops as for 30 min. 1 day postoperatively stress--strain measurements three...

<i>Background:</i> Collagen cross-linking of the cornea has been shown by us to have an antiedematous effect in cornea. The aim present study was examine if this can be used for treatment bullous keratopathy. <i>Methods:</i> This clinical interventional case series included 3 patients (3 eyes) with keratopathy due pseudophakia, corneal transplant rejection, and Fuchs’ endothelial dystrophy. After dehydration 1 day using 40% glucose, central 8 mm were abraded...

Photodynamic collagen cross-linking by using ultraviolet A (UVA) irradiation and the photosensitizer riboflavin has been recently introduced as a new possible treatment of progressive keratoconus. This is first study, to our knowledge, investigating biochemical aspects procedure. Its aim was analyze changes in electrophoretic pattern corneal type I after treatment.Twenty fresh postmortem porcine corneas were cross-linked; another 20 treated with physiologic saline used controls. After...

<h3>Aims</h3> Collagen crosslinking treatment of progressive keratoconus using the photosensitiser riboflavin and ultraviolet A light 370 nm wavelength has been shown to increase significantly tensile strength corneal collagen by about 300%. In keratoconus, interlamellar interfibrillar slippage have proposed as pathogenetic mechanisms. Therefore, aim this study was assess impact on cohesive force. <h3>Methods</h3> 72 post mortem porcine eyes were divided into six different groups: untreated...

From the Department of Ophthalmology, Universitätsklinkum CGC, Dresden, Germany. Reprint requests: Gregor Wollensak, MD, University Eye Clinic Fetscherstr. 74, D-01307 Germany; e-mail: [email protected]

WOLLENSAK, GREGOR MD; SPOERL, EBERHARD PhD; GROSSE, GERNOT WIRBELAUER, CHRISTOPHER MD Author Information