A5013698198- Erythropoietin and Anemia Treatment
- Hormonal and reproductive studies
- Biosimilars and Bioanalytical Methods
- Economic and Social Issues
- Polymer Foaming and Composites
- Blood groups and transfusion
- Mass Spectrometry Techniques and Applications
- Public Administration and Political Analysis
- Blood properties and coagulation
- Environmental Science and Technology
- Erythrocyte Function and Pathophysiology
- Silicone and Siloxane Chemistry
- Medical and Health Sciences Research
- Muscle metabolism and nutrition
- Polymer composites and self-healing
- Ecology, Conservation, and Geographical Studies
- Innovation, Technology, and Society
- Microfluidic and Capillary Electrophoresis Applications
- Pediatric health and respiratory diseases
- Forensic Toxicology and Drug Analysis
- Advanced Proteomics Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Head and Neck Cancer Studies
- Protein purification and stability
- German Literature and Culture Studies
Seibersdorf Laboratories (Austria)
2014-2025
German Sport University Cologne
2013-2025
München Klinik
2021
SMART Reading
2020
University Hospital Münster
2020
University of Dundee
2015
Institute of Bioinformatics and Systems Biology
2015
King's College London
2015
University of Southampton
2015
University of Glasgow
2015
In hepatocellular carcinoma (HCC), intrahepatic metastasis frequently correlates with epithelial to mesenchymal transition (EMT) of malignant hepatocytes. Several mechanisms have been identified be essentially involved in EMT, among them transforming growth factor (TGF)‐β signaling. Here we show the up‐regulation and activation receptor tyrosine kinase Axl EMT‐transformed hepatoma cells. Knockdown expression resulted abrogation invasive transendothelial migratory abilities HCC cells vitro...
High blood pressure is a major contributor to the global burden of disease and discovering novel causal pathways regulation has been challenging. We tested associations with 280 fasting metabolites in 3980 TwinsUK females. Survival analysis for all-cause mortality was performed on significant independent (P<8.9×10(-5)). Replication conducted 2 cohorts KORA (n=1494) Hertfordshire (n=1515). Three animal experiments were establish causality: (1) change after increasing circulating metabolite...
Abstract Doping of athletes with recombinant and genetically modified erythropoietins (EPO) is currently detected by isoelectric focusing (IEF). The application these drugs leads to a significant change in the isoform profile endogenous urinary erythropoietin (uhEPO). Dynepo, MIRCERA, biosimilars variable IEF‐profiles as well active urines effort have made additional testing strategies necessary. new generation small molecule EPO‐receptor stimulating agents like Hematide will also challenge...
Abstract The detection of doping with MIRCERA (the brand name for Continuous Erythropoietin Receptor Activator, or CERA) is hampered by the limited excretion rather large molecule (approximately 60 kDa) in urine. Blood (serum, plasma) combination sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) appears to be ideal matrix detecting all forms erythropoiesis‐stimulating agents (ESAs) because apparent molecular masses ESAs are different from mass human serum erythropoietin...
ABSTRACT A cost minimized immunoaffinity protocol was developed, which allows the direct purification of ERAs (urinary and recombinant human EPO, Darbepoetin, EPO‐Fc, CERA) from urine. The method applies magnetic beads needs no covalent immobilization capture antibody. It requires only 10 mL urine, 1 μg anti‐EPO antibody, 25 μL bead slurry. are coated with antibody in advance can be stored refrigerator for months without any loss functionality. fully validated combination SAR‐PAGE Western...
Die Deutsche Gesellschaft für Humanökologie (DGH) wird 50 Jahre alt. An dieser Stelle richtet sich unser Blick auf die Zukunft. Mit ganz unterschiedlichen Perspektiven theoretische Grundfragen, humanökologische Zielsetzungen, Diskursräume und kritische Selbstreflexion Diskussion eröffnet, der Jahrestagung geführt werden soll.
ABSTRACT The usage of ACE‐031 (Ramatercept), a dimeric fusion protein consisting human activin receptor IIB (ACVR2B) fragment linked to an Fc‐part IgG1, is banned according chapter S4.3 the “WADA 2024 List Prohibited Substances and Methods” due its potential performance enhancing properties. While has not yet been pharmaceutically approved, it sold as research chemical on “black market” (BM). article presents study BM products detection by gel‐electrophoresis Western blotting. Of 14 tested...
Abstract Our experiments show that it is possible to detect different types of recombinant human erythropoietins (rhEPOs), EPO‐ α , β and novel erythropoesis stimulating protein (NESP), based on exact molecular weight (MW) determination by matrix‐assisted laser desorption/ionisation mass spectrometry (MALDI‐MS) applying a high‐resolution time‐of‐flight (TOF) analyser in the linear mode. Detection limits for highly purified, intact glycoproteins were achievable low fmol range (25–50 fmol)...
Several studies suggest that low birthweight resulting from restricted intrauterine growth can leave a metabolic footprint which may persist into adulthood. To investigate this, we performed metabolomic profiling on 5036 female twins, aged 18–80, with weight at birth information available the TwinsUK cohort and independent replication in two additional cohorts. Out of 422 compounds tested, 25 metabolites associated these replicated 1951 men women Hertfordshire Cohort Study (HCS, 66) 2391...
A medical and scientific multidisciplinary consensus meeting was held from 29 to 30 November 2013 on Anti-Doping in Sport at the Home of FIFA Zurich, Switzerland, create a roadmap for implementation 2015 World Code. The statement accompanying papers set out priorities antidoping community research, science medicine. participants achieved strategy Key components this include: (1) sport-specific risk assessment, (2) prevalence measurement, (3) test distribution plans, (4) storage reanalysis,...
Sotatercept (formerly ACE-011) is a glycosylated, dimeric fusion protein composed of the extracellular domain human activin receptor type IIA (ActRIIA) and Fc region IgG1. The protein-based drug candidate acts as ligand trap which competitively binds to A other members transforming growth factor beta superfamily, thus blocking signalling through ActRIIA. Since inhibition was found significantly increase bone formation quality, originally developed for treatment diseases involving loss. But...
The structural characterization of the O- and N-glycan structures three different commercially available recombinant human erythropoietins (rhEPOs) is represented by means a microscale sample purification using ZipTip technology MALDI-TOF MALDI low-energy CID MS. Glycopeptides were released from rhEPO samples differential endoproteolytic digestion to obtain site-specific glycosylation patterns. Mass accuracies in range +/- 0.04% obtained high-resolution TOF instrument allowed an unambiguous...
The neonatal Fc receptor (FcRn) has been under investigation for several years as a pharmaceutical drug target. Clinical studies have shown that fusion proteins consisting of human recombinant erythropoietin (rhEPO) and the Fc‐part IgG can be transported after pulmonary administration via FcRn across airway epithelium to blood stream. So far, no clinically approved formulation EPO‐Fc is available. Since various forms erythropoietins frequently misused by athletes performance‐enhancing...
The detection of doping with recombinant erythropoietins (Epo) by isoelectric focusing (IEF) and Western double blotting strongly relies on the specificity antibody used. Currently a monoclonal mouse (clone AE7A5) is used for that purpose. Despite its excellent sensitivity (amol range) shows some nonspecific binding behavior. However, occurs outside currently pH range evaluating erythropoietin IEF profiles. A shotgun proteomics approach described consisting preparative large-sized carrier...
The only treatment of end-stage renal disease patients undergoing chronic dialysis is kidney transplantation. However, about half graft recipients encounter organ loss within ten years after There emerging evidence that the presence alloreactive antibodies against non-HLA antigens in serum recipient prior transplantation associated with higher incidence rejection. molecular identity these largely unknown. To determine most common antigens, we tested lymphocytic extracts from 20 healthy...
Sequential deglycosylation by exoglycosidase treatment (Reagent Array Analysis Method, RAAM) and subsequent sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) revealed a profound structural difference between human endogenous recombinant erythropoietins. While both proteins behaved similarly upon digestion with Arthrobacter ureafaciens α-sialidase Steptococcus pneumoniae β-D-galactosidase, the action of N-acetyl-β-D-glucosaminidase from was partly blocked but not...
Human erythropoietin (hEPO) is an erythropoiesis stimulating hormone frequently employed in antianemia therapy. Its capability to increase the amount of red blood cells however makes hEPO and its derivatives also attractive dishonest athletes aiming at artificial illicit enhancement their endurance performance. A major objective international antidoping fight elimination drug misuse prevention severe adverse effects caused by nontherapeutic administrations highly potent drugs. The emergence...
Erythropoietin (EPO) is a hormone, which stimulates the production of red blood cells. Due to its performance-enhancing effect, it prohibited by World Anti-Doping Agency (WADA). In order reduce detection window EPO doping, athletes have been applying low doses recombinant (e.g., <10 IU/kg body weight, daily or every second day) instead larger twice more per week 30 IU/kg). Microdoses Retacrit (epoetin zeta), an biosimilar, were administered intravenously and subcutaneously human males...