Bacterial nanocellulose (BNC) has been drawing enormous attention because of its versatile properties. Herein, we shed light on the BNC production by a novel bacterial isolate (MD1) utilizing various agro-industrial wastes. Using 16S rRNA nucleotide sequences, was identified as Komagataeibacter saccharivorans MD1. For first time, synthesis K. MD1 investigated wastes palm date, fig, and sugarcane molasses along with glucose Hestrin-Schramm (HS) medium control. After incubation for 168 h,...

The World Health Organization (WHO) reports that the emergence of multidrug-resistant and slow advent novel more potent antitumor antimicrobial chemotherapeutics continue to be highest concern for human health. Additionally, stability, low solubility, negative effects existing drugs make them ineffective. Studies into alternative tactics tackle such tenacious diseases was sparked by anticancer antibacterial. Silver (Ag) gold (Au) nanoparticles (NPs) were created from Trichoderma...

The textile and leather industry produces large amounts of wastewater that contains high levels chemicals other pollutants, incorporated during the processing stages. We evaluated efficacy green synthesized iron oxide (α-Fe2O3) magnesium (MgO) nanoparticles (NPs) for treatment crude tannery effluents. Green synthesis α-Fe2O3-NPs MgO-NPs was conducted using newly obtained Aspergillus carbonarious strain D-1. optimization physicochemical parameters biosynthesis NPs, including incubation...

Abstract The goal of the current work was to investigate antibacterial, antibiofilm, anticancer, and antioxidant opportunities silver gold nanoparticles (AgNPs AuNPs) synthesized utilizing a new fungus strain called Fusarium pseudonygamai TB-13c. With aid UV, HR-TEM, FTIR, SEM, XRD, NPs’ creation examined. For AgNPs AuNPs, mycosynthesized highest peak plasmon band seen at around 420 540 nm, respectively. ranged in size from 5 20 whereas AuNPs 8 60 nm. were spherical form. AgNPs, angles...

Exopolysaccharide (EPS) biopolymers produced by microorganisms play a crucial role in the environment such as health and bio-nanotechnology sectors, gelling agents food cosmetic industries addition to bio-flocculants environmental sector they are degradable, nontoxic. This study focuses on improvement of EPS production through manipulation different culture conditions using response surface methodology (RSM). Plackett-Burman design indicated that; molasses, yeast extract incubation...

A 205 samples representing eight different infant foods with various based materials were collected and analyzed for their microbiological properties. The contamination rate by aerobic spore formers was achieved 100% in milk food fruit, vegetables, honey, rice powder. While, it detected 95, 60 65% of the wheat based, ready to use (infant fruit) vegetables), respectively. Biochemical Identification API 50 CHB used identify obtained isolates revealed that B. subtilis most frequently occurring...

Optically pure lactic acid (LA) is an important chemical platform that has a wide range of industrial and biotechnological applications. Improved parameters for cost effective LA production are great interest developments. In the present study, alkaliphilic bacterium, BoM 1-2, was selected among 369 newly obtained bacterial isolates. It characterized using API 50 CHL kit identified as Enterococcus hirae 1-2 by 16S rRNA gene sequence analysis. Efficient polymer-grade L -lactic achieved at pH...

Abstract In the current study, optimal reaction condition for fabrication of Au and Ag nanoparticles by using Trichoderma saturnisporum was developed. Optimization fermentation conditions extracellular AuNPs AgNPs synthesis response surface methodology achieved. To accomplish ideal state, green reaction’s effective parameters central composite face design were used. Precursor concentrations, pH, temperature found to be most nanomaterials. The model’s realization used a respectable foot....

The present work was designed for studying the efficiency of multi-factorial experimental designs to elucidate factors affecting exopolysaccharide production by K.oxytoca isolated from soil.Combinations Plackett-Burman design (PBD) and Box -Behnken (BBD) have been carried out media optimization various factors.Among 11 factors, yeast extract, molasses incubation temperature were most significant factors.These three selected further using Box-Behnken (BBD).The result obtained data analysis...

The industrial wastewater (WW) of potato-chips factory is characterized by its high biological oxygen demand (BOD) and chemical (COD), in addition to a medium content oil & grease (O&G), total dissolved slats (TDS) suspended solids (TSS). A new technique for treatment has been applied using bio-mixture selected strains Aspergillus terreus or Rhizopus sexualis the natural flora sawdust (SD-BIOMIX) form mobile micro-carrier activated sludge system. Different kinds composted were used as...

Egypt produces tons of date palm fibers every year. Fibers Phoenix dactylifera were chemically pulped in here by alkali hydrolysis into hemicellulose, wax, lignin etc... Fractions produced characterized via spectroscopic techniques, while pure cellulose was examined XRD and 1H NMR. Finally, crude designed a microcrystalline form acid for much better surface merits supporting the acetylation reaction resulting acetate (CA). Using NMP as solvent, casting solutions CA prepared loaded with...

Abstract Foodborne pathogens can be found in various foods, and it is important to detect foodborne provide a safe food supply prevent diseases. The nucleic acid base detection method one of the most rapid widely used methods pathogens; depends on hybridizing target sequence synthetic oligonucleotide (probes or primers) that complementary sequence. Designing primers probes for this preliminary critical step. However, new bioinformatics tools are needed automate, specific improve design sets...

The aim of this study was to isolate and identify lipolytic bacteria. Perform a statistical stepwise physicochemical optimization for maximum production extracellular lipase its validation in bioreactor. Several bacteria were isolated from petroleum hydrocarbon‐polluted soil. strain expressing the highest activity (47 U ml−1) genetically identified as Gram‐positive Bacillus stratosphericus PSP8 (NCBI GenBank accession no. MH120423). response surface methodology (RSM)–central composite face...

High cell density fermentation strategies for the production of bovine lactoferrin in Pichia pastoris Thuy Thi Thu Trinh, Huong Ngo, Phong Quoc Truong

The major drawback of the wide applicability biodiesel is its price compared to conventional petro-diesel. feedstock and applied catalyst in transesterification reaction are main contributor for overall cost production. Thus, this study summarizes optimization a batch used domestic waste oil (UDWO) with methanol using CaO, which can be easily prepared from different cheap readily available natural sources. Quadratic model equations were elucidated describing effect methanol:oil molar ratio,...

In this study, response surface methodology based on face center composite – 1/2 factorial fraction design (FCCD) of experiments was employed to optimize the batch transesterification process waste cooking oil as one most abundant and readily available domestic wastes with methanol using immobilized Candida antarctica lipase (Novozym435) maximize production high-purity biodiesel. Statistically significant second-order quadratic model equations (p < 0.0001) were elucidated through multiple...