A5055166215- Nanoplatforms for cancer theranostics
- Advanced biosensing and bioanalysis techniques
- Photoacoustic and Ultrasonic Imaging
- SARS-CoV-2 detection and testing
- Gold and Silver Nanoparticles Synthesis and Applications
- RNA Interference and Gene Delivery
- Biosensors and Analytical Detection
- Luminescence and Fluorescent Materials
- SARS-CoV-2 and COVID-19 Research
- Antimicrobial Peptides and Activities
- Advanced Nanomaterials in Catalysis
- Polymer Surface Interaction Studies
- Conducting polymers and applications
- Photodynamic Therapy Research Studies
- Molecular Junctions and Nanostructures
- Nanocluster Synthesis and Applications
- Herpesvirus Infections and Treatments
- Advanced Polymer Synthesis and Characterization
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Extracellular vesicles in disease
- CRISPR and Genetic Engineering
- Nanoparticle-Based Drug Delivery
University of California, San Diego
2020-2024
La Jolla Bioengineering Institute
2022
University of California System
2020
Abstract The transmission of SARS‐CoV‐2 coronavirus has led to the COVID‐19 pandemic. Nucleic acid testing while specific limitations for mass surveillance. One alternative is main protease (M pro ) due its functional importance in mediating viral life cycle. Here, we describe a combination modular substrate and gold colloids detect M via visual readout. strategy involves zwitterionic peptide that carries opposite charges at C‐/N‐terminus exploit recognition by . Autolytic cleavage releases...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a serious threat to human health and lacks an effective treatment. There urgent need for both real-time tracking precise treatment of the SARS-CoV-2-infected cells mitigate ultimately prevent viral transmission. However, selective triggering therapeutic process in infected remains challenging. Here, we report main protease (Mpro)-responsive, mitochondrial-targeting, modular-peptide-conjugated probe (PSGMR) imaging inhibition via...
Plant viral nanoparticles (plant VNPs) are promising biogenetic nanosystems for the delivery of therapeutic, immunotherapeutic, and diagnostic agents. The production plant VNPs is simple highly scalable through molecular farming in plants. Some important advances VNP nanotechnology include genetic modification, disassembly/reassembly, bioconjugation. Although effective, these methods often involve complex time-consuming multi-step protocols. Here, we report a versatile supramolecular coating...
Activatable contrast agents are of ongoing research interest because they offer low background and high specificity to the imaging target. Engineered sensitivity protease activity is particularly desirable proteases critical biomarkers in cancer, infectious disease, inflammatory disorders, so forth. Herein, we developed characterized a set peptide-linked cyanine conjugates for dual-modal detection via photoacoustic (PA) fluorescence imaging. The peptide–dye were designed undergo contact...
The main protease (M
Abstract A longstanding problem with conventional cancer therapy is the nonspecific distribution of chemotherapeutics. Monitoring drug release in vivo via noninvasive bioimaging can thus have value, but it difficult to distinguish loaded from released live tissue. Here, this work describes an injectable supramolecular hydrogel that allows slow and trackable doxorubicin (Dox) photoacoustic (PA) tomography. Dox covalently linked methylene blue (MB) monitor before, during, after carrier. The...
Abstract While vaccines have been highly successful in protecting against various infections, there are still many high‐priority pathogens for which no clinically approved formulations. To overcome this challenge, researchers explored the use of nanoparticulate strategies more effective antigen delivery to immune system. Along these lines, nanotoxoids a promising biomimetic platform that leverages cell membrane coating technology safely deliver otherwise toxic bacterial antigens their native...
Photoacoustic (PA) imaging has proved versatile for many biomedical applications from drug delivery tracking to disease diagnostics and postoperative surveillance. It recently emerged as a tool accurate real-time heparin monitoring avoid bleeding complications associated with anticoagulant therapy. However, molecular-dye-based application is limited by high concentration requirements, photostability, strong background hemoglobin signal. We developed polydopamine nanocapsules (PNCs) via...
Abstract We report noncovalent assemblies of iRGD peptides and methylene blue dyes via electrostatic hydrophobic stacking. These resulting nanomaterials could bind to cancer cells, image them with photoacoustic signal, then treat photodynamic therapy. first assessed the optical properties physical materials. evaluated their utility for live cell targeting, in vivo imaging, toxicity. tuned performance by adding aspartic acid (DD) or tryptophan doublets (WW) peptide promote stacking blue,...
Organic nanomaterials increasingly generate interest from diverse fields spanning fundamental and clinical research, biotechnology, nanomedicine. are exceptionally versatile, with applications in several domains: molecular imaging, pharmaceutical formulations, image-guided therapies. Unlike inorganic nanomaterials, these materials offer distinct advantages, such as facile tunability composition or surface functionalization, excellent biocompatibility, controlled release, so on. Here,...
Gold nanorods (GNRs) have attracted great interest for photo-mediated biomedicines due to their tunable and high optical absorption, photothermal conversion efficiency facile surface modifiability.GNRs that efficient absorption in second near-infrared (NIR-II) window hold further promise bio-applications low background signal from tissue deep penetration.However, bare GNRs readily undergo shape deformation (termed as 'melting effect') during the laser illumination losing unique localized...
We report the reversible aggregation of gold nanoparticles (AuNPs) assemblies via a di-arginine peptide additive and thiolated PEGs (HS-PEGs). The AuNPs were first aggregated by attractive forces between citrate-capped surface arginine side chains. found that HS-PEG thiol group has higher affinity for AuNP surface, thus leading to redispersion colloidal stability. In turn, there was robust obvious color change due on/off plasmonic coupling. assemblies' dissociation directly related...
Polymer nanocapsules have demonstrated significant value in materials science and biomedical technology, but require complicated time-consuming synthetic steps. We report here the facile synthesis of monodisperse polymer via a redox-mediated kinetic strategy from two simple molecules: dopamine benzene-1,4-dithiol (BDT). Specifically, BDT forms core templates modulates oxidation kinetics into polydopamine (PDA) shells. These uniform nanoparticles can be tuned between ≈70 200 nm because...
Transmembrane protease serine 2 (TMPRSS2) is a plasma membrane that activates both spike protein of coronaviruses for cell entry and oncogenic signaling pathways tumor progression. TMPRSS2 inhibition can reduce cancer invasion metastasis partially prevent the SARS-CoV-2 into host cells. Thus, there an urgent need TMPRSS2-selective imaging precise screening inhibitors. Here, we report TMPRSS2-responsive surface-potential-tunable peptide-conjugated probe (EGTP) with aggregation-induced...
Nucleic acids are well-established biomarkers of cancer with immense value in diagnostics and basic research. However, strategies to monitor these species tissue can be challenging due the need for amplification imaging signal from low analyte concentrations high specificity. Photoacoustic (PA) is gaining traction molecular proteins, small biomolecules, nucleic by coupling pulsed near-infrared (NIR) excitation broadband acoustic detection. This work introduces a PA acid contrast agent that...
Abstract The transmission of SARS‐CoV‐2 coronavirus has led to the COVID‐19 pandemic. Nucleic acid testing while specific limitations for mass surveillance. One alternative is main protease (M pro ) due its functional importance in mediating viral life cycle. Here, we describe a combination modular substrate and gold colloids detect M via visual readout. strategy involves zwitterionic peptide that carries opposite charges at C‐/N‐terminus exploit recognition by . Autolytic cleavage releases...
Abstract The main protease (M pro ) and papain‐like (PL play critical roles in SARS‐CoV‐2 replication are promising targets for antiviral inhibitors. simultaneous visualization of M PL is extremely valuable detection rapid inhibitor screening. However, such a crucial investigation has remained challenging because the lack suitable probes. We have now developed dual‐color probe (3MBP5) by fluorescence (or Förster) resonance energy transfer (FRET). This produces from both Cy3 Cy5 fluorophores...
Abstract Polymer nanocapsules have demonstrated significant value in materials science and biomedical technology, but require complicated time‐consuming synthetic steps. We report here the facile synthesis of monodisperse polymer via a redox‐mediated kinetic strategy from two simple molecules: dopamine benzene‐1,4‐dithiol (BDT). Specifically, BDT forms core templates modulates oxidation kinetics into polydopamine (PDA) shells. These uniform nanoparticles can be tuned between ≈70 200 nm...
Tumor-targeting contrast agents promise indispensable value for therapeutic monitoring, image guided therapy, early stage detection, and tumor growth surveillance. Many agents, however, are limited by millimeter-ranged penetration depths, ionizing radiation sources, full-body scan imaging modalities, or a short half-life. Photoacoustic ultrasound offers chairside real-time platform using both endogenous exogenous sources optical contrast. In this work, we formulated micelle-inspired...
ABSTRACT We report the reversible aggregation of gold nanoparticle (AuNPs) assemblies via a diarginine peptide additive and thiolated PEGs (HS-PEGs). The AuNPs were first aggregated by attractive forces between citrate-capped surface arginine side chains. found that HS-PEG thiol group has higher affinity for surface, thus leading to redispersion colloidal stability. In turn, there was robust obvious color change due on/off plasmonic coupling. assemblies’ dissociation directly related...
DNA nanostructures are well-established vectors for packaging diversified payloads targeted cellular delivery. Here, origami rectangular sheets were combined with Herpes Simplex Virus 1 (HSV1) capsids to demonstrate surface coverage of the particle via electrostatic interactions. The optimized origami:HSV1 molar ratios led characteristic geometries ranging from dispersed "HSV1 pockets" agglomerated sleeves". "Pockets" disguised cells in HeLa and B16F10 44.2% less infective than naked HSV1...