A5047455168- Ion channel regulation and function
- Urinary Bladder and Prostate Research
- Cardiac electrophysiology and arrhythmias
- Cellular transport and secretion
- Neuroscience of respiration and sleep
- Receptor Mechanisms and Signaling
- Lipid Membrane Structure and Behavior
- Neuroscience and Neuropharmacology Research
- Ion Channels and Receptors
- Protein Kinase Regulation and GTPase Signaling
- Calcium signaling and nucleotide metabolism
- Pelvic floor disorders treatments
- Cardiomyopathy and Myosin Studies
- Pain Mechanisms and Treatments
- Photoreceptor and optogenetics research
- Pancreatic function and diabetes
- Real-time simulation and control systems
- Migraine and Headache Studies
- Heart Failure Treatment and Management
- Vestibular and auditory disorders
- Spinal Cord Injury Research
- Political and Social Issues
- Religion, Theology, and Education
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
Bates College
2019-2024
University of Washington
2012-2017
Seattle University
2013-2017
Universität Hamburg
2009-2012
Bernhard Nocht Institute for Tropical Medicine
2005-2012
University Medical Center Hamburg-Eppendorf
2011-2012
Hospices Civils de Lyon
2010
Université Claude Bernard Lyon 1
2010
Rockefeller University
2005
University of Pittsburgh
1990-1995
Progressive familial heart block type I (PFHBI) is a progressive cardiac bundle branch disease in the His-Purkinje system that exhibits autosomal-dominant inheritance. In 3 branches of large South African Afrikaner pedigree with an form PFHBI, we identified mutation c.19G-->A transient receptor potential cation channel, subfamily M, member 4 gene (TRPM4) at chromosomal locus 19q13.3. This predicted amino acid substitution p.E7K TRPM4 terminus. encodes Ca2+-activated nonselective (CAN)...
Isolated cardiac conduction block is a relatively common condition in young and elderly populations. Genetic predisposing factors have long been suspected because of numerous familial case reports. Deciphering genetic blocks may give hint at stratifying carriers more efficient way.One Lebanese family 2 French families with autosomal dominant isolated were used for linkage analysis. A maximum combined multipoint lod score 10.5 was obtained on genomic interval including than 300 genes. After...
No AccessJournal of UrologyInvestigative Urology1 Jun 1995Neural Control Urethral Outlet Activity in Vivo: Role Nitric Oxide B.C. Bennett, M.N. Kruse, J.R. Roppolo, H.D. Flood, M. Fraser, and W.C. de Groat BennettB.C. Bennett , KruseM.N. Kruse RoppoloJ.R. Roppolo FloodH.D. Flood FraserM. Fraser GroatW.C. View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)67391-9AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked...
Spinal cord injury (SCI) in humans results inappropriate contractions of the external urethral sphincter muscle (EUS) during micturition (bladder-sphincter dyssynergia), leading to urinary retention. The major goal this study was determine whether SCI rats has similar detrimental effects on micturition. After chronic SCI, urethan-anesthetized had a significantly (15-fold) increased bladder capacity and impaired voiding (31-fold increase residual volume) compared with control rats. Bladder...
Endoplasmic reticulum–plasma membrane (ER–PM) contact sites play an integral role in cellular processes such as excitation–contraction coupling and store-operated calcium entry (SOCE). Another ER–PM assembly is one tethered by the extended synaptotagmins (E-Syt). We have discovered that at steady state, E-Syt2 positions ER Sac1, lipid phosphatase, discrete junctions. Here, Sac1 participates phosphoinositide homeostasis limiting PM phosphatidylinositol 4-phosphate (PI(4)P), precursor of...
DNA methylation is known as the prima donna epigenetic mark for its critical role in regulating local gene transcription. Changes landscape of across genome occur during cellular transition, such differentiation and altered neuronal plasticity, become dysregulated disease states cancer. The TET family enzymes to be responsible catalyzing reverse process that demethylation by recognizing 5-methylcytosine oxidizing methyl group via an Fe(II)/alpha-ketoglutarate-dependent mechanism. Here, we...
Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) regulates activities of numerous ion channels including inwardly rectifying potassium (Kir) channels, KCNQ, TRP, and voltage-gated calcium channels. Several studies suggest that (KV) might be regulated by PI(4,5)P2. Wide expression KV in different cells suggests such regulation could have broad physiological consequences. To study PI(4,5)P2, we coexpressed several them tsA-201 with a G protein–coupled receptor (M1R), voltage-sensitive lipid...
Myo-inositol is an important cellular osmolyte in autoregulation of cell volume and fluid balance, particularly for mammalian brain kidney cells. We find it also regulates excitability. the precursor phosphoinositides, key signaling lipids including phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2]. However, whether myo-inositol accumulation during osmoregulation affects excitability has not been fully explored. found that overexpression Na(+)/myo-inositol cotransporter (SMIT1)...
Cardiac atrial natriuretic peptide (ANP) locally counteracts cardiac hypertrophy via the guanylyl cyclase-A (GC-A) receptor and cGMP production, but downstream signalling pathways are unknown. Here, we examined influence of ANP on β-adrenergic versus Angiotensin II (Ang II)-dependent (Gs vs. Gαq mediated) modulation Ca2+ i-handling in cardiomyocytes intact hearts. L-type currents i transients adult isolated murine ventricular myocytes were studied by voltage-clamp recordings fluorescence...
Leishmania major aquaglyceroporin (LmjAQP1) adventitiously facilitates the uptake of antimonite [Sb(III)], an active form Pentostam® or Glucantime®, which are first line defence against all forms leishmaniasis. The present paper shows that LmjAQP1 activity is modulated by mitogen-activated protein kinase, LmjMPK2. parasites coexpressing and LmjMPK2 show increased Sb(III) sensitivity. When subjected to a hypo-osmotic stress, these cells faster volume recovery than expressing alone....
Phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] is essential for exocytosis. Classical ways of manipulating PI(4,5)P2 levels are slower than its metabolism, making it difficult to distinguish effects from those metabolites. We developed a membrane-permeant, photoactivatable PI(4,5)P2, which loaded into cells in an inactive form and activated by light, allowing sub-second increases levels. By combining this compound with electrophysiological measurements mouse adrenal chromaffin cells, we...
Cardiac atrial natriuretic peptide (ANP) regulates arterial blood pressure, moderates cardiomyocyte growth, and stimulates angiogenesis metabolism. ANP binds to the transmembrane guanylyl cyclase (GC) receptor, GC-A, exert its diverse functions. This process involves a cGMP-dependent signaling pathway preventing pathological [Ca 2+ ] i increases in myocytes. In chronic cardiac hypertrophy, however, levels are markedly increased GC-A/cGMP responses blunted due receptor desensitization. Here...
Significance Phosphatase and tensin homolog (PTEN) is a phosphatidylinositol 3,4-bisphosphate [PI(3,4)P 2 ] 3,4,5-trisphosphate [PI(3,4,5)P 3 3-phosphatase that plays important roles in cell polarization, division, development as well tumor suppression. Voltage-sensing phosphatases (VSPs) are PTEN family members but have catalytic activity response to membrane depolarization with wider range of substrates: They cleave the 5-phosphate PI(3,4,5)P 4,5-bisphosphate 3-phosphate PI(3,4)P perhaps ....
Micturition in neonatal rats is mediated by a spinal reflex pathway activated the mother licking perineum (the perineal-to-bladder reflex, P-Bld). adult spinobulbospinal bladder distension bladder-to-bladder Bld-Bld). This study examines postnatal development of Bld-Bld decerebrate or spinalized unanesthetized and urethan-anesthetized rat pups 2-26 days age. Urethan anesthesia depressed both P-Bld micturition reflexes. reflexes (peak intravesical pressures 13 +/- 6 cmH2O durations 35 11 s)...
Leishmania parasites undergo profound morphological and biochemical changes while passing through their life cycle. Protein kinases have been shown to be involved in the differentiation from extracellular flagellated promastigotes intracellular "non-flagellated" amastigotes vice versa. Moreover, these enzymes are likely regulation of proliferation different stages.Here, we characterize LmxMPK4, a mitogen-activated protein (MAP) kinase homologue mexicana. The reveals all sequence motifs for...
Levels of nerve growth factor (NGF) are elevated in inflamed tissues. In sensory neurons, increases NGF augment neuronal sensitivity (sensitization) to noxious stimuli. Here, we hypothesized that also sensitizes sympathetic neurons proinflammatory We cultured superior cervical ganglion (SCG) from adult male Sprague Dawley rats with or without added and compared their responsiveness bradykinin, a peptide. The NGF-cultured exhibited significant depolarization, bursts action potentials, Ca 2+...