A5080185074- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Streptococcal Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Escherichia coli research studies
- Reproductive tract infections research
- Antimicrobial Resistance in Staphylococcus
- Respiratory viral infections research
- Infective Endocarditis Diagnosis and Management
- Salmonella and Campylobacter epidemiology
- Syphilis Diagnosis and Treatment
- Botany, Ecology, and Taxonomy Studies
- Fungal Biology and Applications
- Child Nutrition and Water Access
- Pneumocystis jirovecii pneumonia detection and treatment
- Plant Diversity and Evolution
- Bacterial Identification and Susceptibility Testing
- Neonatal and Maternal Infections
- Plant and animal studies
- Botany and Plant Ecology Studies
- Clostridium difficile and Clostridium perfringens research
- Mycorrhizal Fungi and Plant Interactions
- Botanical Studies and Applications
- Diphtheria, Corynebacterium, and Tetanus
- Virology and Viral Diseases
Queensland Health
2012-2023
Government of Western Australia Department of Health
2014-2023
Australian Government
2023
Massey University
2021
Scientific Services
2005-2019
Public Health England
2018
The University of Queensland
2017
Queensland Government
2017
Pfizer (United Kingdom)
2015
Queensland Police Service
2015
Pneumococcal polysaccharide conjugate vaccines prevent pneumococcal disease in infants, but their efficacy against community-acquired pneumonia adults 65 years of age or older is unknown.In a randomized, double-blind, placebo-controlled trial involving 84,496 older, we evaluated the 13-valent vaccine (PCV13) preventing first episodes vaccine-type strains pneumonia, nonbacteremic and noninvasive invasive disease. Standard laboratory methods serotype-specific urinary antigen detection assay...
A new variant of Streptococcus pyogenes serotype M1 (designated 'M1UK') has been reported in the United Kingdom, linked with seasonal scarlet fever surges, marked increase invasive infections, and exhibiting enhanced expression superantigen SpeA. The progenitor S. 'M1global' M1UK clones can be differentiated by 27 SNPs 4 indels, yet mechanism for speA upregulation is unknown. Here we investigate previously unappreciated expansion Australia, now isolated from majority serious infections...
We determined the incidence of cryptosporidiosis in children aged <5 years presenting with diarrhoea an urban and rural hospital-based setting Malawi. Stools were collected over a 22-month period during both rainy dry seasons. A range microscopic methods used to determine presence Cryptosporidium spp. oocysts. Species determination was by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) oocyst-extracted DNA using 18S rRNA COWP gene loci. oocysts seen 5.9%...
A novel method for genotyping the clustered, regularly interspaced short-palindromic-repeat (CRISPR) locus of Campylobacter jejuni is described. Following real-time PCR, CRISPR products were subjected to high-resolution melt (HRM) analysis, a new technology that allows precise profile determination amplicons. This investigation shows HRM assay provides powerful addition existing C. methods and emphasizes potential short sequence repeats in other species.
Neisseria gonorrhoeae antimicrobial resistance (AMR) is a global problem heightened by emerging to ceftriaxone. Appropriate molecular typing methods are important for understanding the emergence and spread of N. AMR. We report on development, validation testing Sequenom MassARRAY iPLEX method multilocus sequence (MLST)-style genotyping isolates. An (iPLEX14SNP) was developed targeting 14 informative gonococcal single nucleotide polymorphisms (SNPs) previously shown predict MLST types. The...
ABSTRACT Escherichia coli O157 is a food-borne pathogen whose major reservoir has been identified as cattle. Recent genetic information indicated that populations of E. from cattle and humans can differ genetically this variation may have an impact on their ability to cause severe human disease. In addition, there emerging evidence strains different geographical regions also be divergent. To investigate the extent variation, we used Shiga toxin bacteriophage insertion sites (SBI),...
During a large outbreak of Shiga toxin-producing Escherichia coli illness associated with an agricultural show in Australia, we used whole-genome sequencing to detect IS1203v insertion the toxin 2c subunit A gene E. coli. Our study showed that clinical was mild, and hemolytic uremic syndrome not detected.
SUMMARY We investigated an outbreak of Shigella sonnei infections in Denmark and Australia associated with imported baby corn from one packing shed Thailand. reviewed nationwide surveillance undertook case finding, food trace-back microbiological investigation human, environmental samples. A recall sugar snaps was based on descriptive epidemiological evidence. In Denmark, we a retrospective cohort study workplace. total, 215 cases were laboratory-confirmed 12 Australia. multivariable...
ABSTRACT While the differential association of Escherichia coli O157 genotypes with animal and human hosts has recently been well documented, little is known about their distribution between countries how this might affect regional disease rates. Here, we used a 48-plex single nucleotide polymorphism (SNP) assay to segregate 148 E. isolates from Australia, Argentina, United States into 11 SNP lineages. We also investigated relationship lineages, Shiga toxin (Stx) gene profiles, total Stx...
Background. Antimicrobial resistance (AMR) by Neisseria gonorrhoeae is considered a serious global threat. Methods. In this nationwide study, we used MassARRAY iPLEX genotyping technology to examine the epidemiology of N. and associated AMR in Australian population. All available isolates (n = 2452) received from reference laboratories January June 2012 were included study. Genotypic data combined with phenotypic information define strain types. Results. A total 270 distinct types observed....
We investigated the prevalence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli in untreated hospital wastewaters and 2 sewage treatment plants (STPs). A collection 252 ESBL-producing E. isolates from wastewater STPs were typed tested for resistance to 17 antimicrobial agents presence integron-associated integrases (intI gene) ESBL genes. Eighty-nine percent (n = 176) strains found more than 1 sample (common types), with common type accounting 35% isolates, all samples....
SUMMARY Following the introduction of vaccination against Haemophilus influenzae type b (Hib), cases invasive encapsulated Hib disease have decreased markedly. This study aimed to examine subsequent epidemiological trends in H. Queensland, Australia and particular, assess clinical impact public health implications non-typable (NTHi) strains. A multicentre retrospective was conducted from July 2000 June 2013. Databases major laboratories Queensland including Forensic Scientific Services...
The objective of this study was to develop a real-time PCR method for specific detection the gonococcal GyrA amino acid 91 locus directly in clinical samples so as predict Neisseria gonorrhoeae ciprofloxacin susceptibility.The assay, GyrA91-PCR, designed using two probes, one WT S91 sequence and other S91F alteration. performance assay initially assessed characterized N. isolates (n = 70), panel commensal Moraxella species 55 isolates) providing negative results by commercial nucleic...
ObjectivesSurveillance for Neisseria gonorrhoeae azithromycin resistance is of growing importance given increasing use ceftriaxone and dual therapy gonorrhoea treatment. In this study, we developed two real-time PCR methods direct detection key N. 23S rRNA mutations associated with resistance.
Diphtheria is a potentially fatal respiratory disease caused by toxigenic Corynebacterium diphtheriae. Although resistance to erythromycin has been recognized, β-lactam in diphtheria not described. Here, we report case of C. diphtheriae resistant penicillin and all other antibiotics, describe novel mechanism inducible carbapenem associated with the acquisition mobile element.Long-read whole-genome sequencing was performed using Pacific Biosciences Single Molecule Real-Time determine genome...