A5101473206- Neurobiology and Insect Physiology Research
- Nicotinic Acetylcholine Receptors Study
- Lipid metabolism and biosynthesis
- Photoreceptor and optogenetics research
- Cellular transport and secretion
- Neuroscience and Neuropharmacology Research
- Cell Image Analysis Techniques
- Insect Utilization and Effects
- Adipose Tissue and Metabolism
- Neural dynamics and brain function
- Invertebrate Immune Response Mechanisms
- Advanced Fluorescence Microscopy Techniques
- Lipid Membrane Structure and Behavior
- Endoplasmic Reticulum Stress and Disease
- Plant Molecular Biology Research
- Anesthesia and Neurotoxicity Research
National Institute of Neurological Disorders and Stroke
2018-2025
National Institutes of Health
2015-2025
Morpho (United States)
2018
Abstract Lipid shuttling between neurons and glia contributes to the development, function, stress responses of nervous system. To understand how a neuron acquires its lipid supply from specific lipoproteins their receptors, we perform combined genetic, transcriptome, biochemical analyses in developing Drosophila larval brain. Here report, astrocyte-derived secreted lipocalin Glial Lazarillo (GLaz), homolog human Apolipoprotein D (APOD), neuronal receptor, brain-specific short isoforms...
Highly motile dendritic protrusions are hallmarks of developing neurons. These exploratory filopodia sample the environment and initiate contacts with potential synaptic partners. To understand role for dynamic in dendrite morphogenesis experience-dependent structural plasticity, we analyzed dynamics, synapse formation, volume expansion ventral lateral neurons (LNvs) Drosophila larval visual circuit. Our findings reveal temporal coordination between heightened dynamics synaptogenesis LNvs...
Synapses have undergone significant diversification and adaptation, contributing to the complexity of central nervous system. Understanding their molecular architecture is essential for deciphering brain’s functional evolution. While nicotinic acetylcholine receptors (nAchRs) are widely distributed across metazoan brains, associated protein networks remain poorly characterized. Using in vivo proximity labeling, we generated proteomic maps subunit-specific nAchR interactomes developing mature...
Abstract Glia derived secretory factors play diverse roles in supporting the development, physiology, and stress responses of central nervous system (CNS). Through transcriptomics imaging analyses, we have identified Obp44a as one most abundantly produced proteins from Drosophila CNS glia. Protein structure homology modeling Nuclear Magnetic Resonance (NMR) experiments reveal a fatty acid binding protein (FABP) with high affinity towards long-chain acids both native oxidized forms. Further...
Highlights•Cell-type-specific RNA-seq analyses identify activity modified transcripts•Activity induces the upregulation of LpR1 and LpR2 transcripts in larval LNvs•LpRs are required for LNv dendrite morphogenesis during development•Upregulation LpRs supports neuronal adaptation to elevated activitySummaryActivity-dependent modifications strongly influence neural development. However, molecular programs underlying their context circuit-specific effects not well understood. To study global...
Significance Cholinergic neurotransmission in the central nervous system contributes immensely to animal behavior and is linked a multitude of neuropsychiatric disorders humans. However, contrast well-established role glutamate receptor signaling developing brain, it remains unclear whether mediated by acetylcholine receptors also major effector neural development plasticity. Here, we address this question Drosophila system, where most excitatory synapses use as main neurotransmitter. Our...
Abstract Construction and maturation of the postsynaptic apparatus are crucial for synapse dendrite development. The fundamental mechanisms underlying these processes most often studied in glutamatergic central synapses vertebrates. Whether same principles apply to excitatory cholinergic insect nervous system (CNS) is not known. To address this question, we investigated Drosophila ventral lateral neurons (LNvs) identified nAchRα1 (Dα1) nAchRα6 (Dα6) as main functional nicotinic acetylcholine...
Highly motile dendritic filopodia are widely present in neurons at early developmental stages. These exploratory dynamic branches sample the surrounding environment and initiate contacts with potential synaptic partners. Although connection between branch dynamics synaptogenesis is well established, how activity-dependent processes regulate not understood. This partly due to technical difficulties associated live imaging quantitative analyses of these fine structures using an vivo system. We...
Presentation of poster 2319C at TAGC 2020 Online. Files include a PDF the Developmental switching nicotinic acetylcholine receptor subunits supports central cholinergic synapse maturation.pdf.