Abstract Determining the structure of a protein and its transformation under different conditions is key to understanding activity. The structural stability activity proteins in aqueous–organic solvent mixtures, which an intriguing topic research biochemistry, dependent on nature properties medium. Herein, effect commonly used cosolvent, dimethyl sulfoxide (DMSO), conformational dynamics bovine serum albumin (BSA) studied by fluorescence correlation spectroscopy (FCS) measurements...

Carbon dots are one of the most promising nanomaterials which exhibit a wide range applications in field bioimaging, sensing and biomedicine due to their ultra-small size, high photostability, tunable fluorescence, electrical properties, etc. However, green carbon synthesized from several natural renewable sources show some additional advantages, such as favorable biocompatibility, sources, low cost production ecofriendly nature. In this review, we will provide an update on latest research...

Choline dioctylsulfosuccinate [Cho][AOT] (a surface active ionic liquid) has been found to induce all-α α + β conformational transition in the secondary structure of enzyme cytochrome<italic>c</italic>(Cyt<italic>c</italic>) with an enhanced peroxidase activity its aqueous vesicular phase at pH 7.0.

Laccase is a highly versatile biocatalyst with broad applications across bioremediation, biofuel production, organic synthesis and in industries such as food paper manufacturing. However, the industrial use of laccase often limited by its inactivation under harsh conditions, including presence non-aqueous solvents, extreme pH high temperatures. To overcome these challenges, various solvent engineering strategies have been explored, natural deep eutectic solvents (NADESs) environmentally...

Over the past decade, there has been a significant increase in discovery of greener solvents for industrial applications. In this context, deep eutectic (DESs) have emerged as promising candidates across various sectors, including biomass conversion, paper and pulp, pharmaceuticals, textiles. A new class DESs, known natural (NADESs), is derived from chemicals expected to be more environmentally friendly. However, research into environmental impact toxicity NADESs still limited. Given broad...

The structural integrity of a nucleic acid under various conditions determines its utility in biocatalysis and biotechnology. Exploration the ionic liquids (ILs) for extraction DNA other based applications requires an understanding nature interaction between IL DNA. Considering these aspects, we have studied calf-thymus less toxic morpholinium IL, [Mor1,2][Br], employing fluorescence correlation spectroscopy (FCS), conventional steady state time-resolved fluorescence, circular dichroism (CD)...

The understanding of the transport drugs and naturally occurring molecules in living cells tissues requires a thorough knowledge diffusion behaviour molecular systems these media. In this work, we studied translational three fluorescent molecules, electrically neutral coumarin 102 (C102), cationic rhodamine 6G (R6G) anionic fluorescein (FL) phosphate-buffered (pH 7) aqueous solutions bovine serum albumin (BSA) protein absence presence common salt urea using fluorescence correlation...

Photophysical properties of a natural plant alkaloid, ellipticine (5,11-dimethyl-6H-pyrido[4,3-b]carbazole), which comprises both proton donating and accepting sites, have been studied in different solvents using steady state time-resolved fluorescence techniques primarily to understand the origin dual that this molecule exhibits some specific alcoholic solvents. Ground excited calculations based on density functional theory also carried out help interpretation experimental data. It is shown...

Aggregation behavior of a series neat 1-ethyl 3-methylimidazolium alkyl sulfate (alkyl = ethyl, butyl, hexyl, and octyl) ionic liquids has been investigated through combined time-resolved fluorescence spectroscopy, 1-D 2-D NMR correlation spectroscopy (FCS). Interestingly, experimentally measured rotational relaxation times (τr) for hexyl octyl systems are to be 2.25, 1.64, 1.36, 1.32 higher than the estimated (from Stokes–Einstein–Debye theory) values same respective systems. This indicates...

Ability of the ionic liquids to alter structural stability proteins in aqueous solution is a topic considerable interest modern bioscientific research because possible applications these substances protein purification and as refolding agents. A few early studies involving imidazolium have demonstrated their role both denaturants As influence an liquid on given depends identity species, it necessary extend wider number obtain insight into mechanism interaction between two arrive at...

Abstract Two new free‐base β‐octa and hexaalkyl naphthobipyrrole‐derived sapphyrins are reported along with various salts thereof. One of them has substituents at all its β positions, whereas the pyrrole unit opposite to bipyrrolic moiety is unsubstituted in other. The effect bipyrrole fusion on structure was explored. Interestingly, an unprecedented sandwiched supramolecular aqua‐bridged sapphyrin dimer also characterized solid state. Further, anions third‐order nonlinear optical properties...

Owing to their potential applications in the extraction, purification, and preservation of biomolecules biocatalysis, ionic liquids (ILs) have gained great attention biotechnology. Although it is known that structure dynamics proteins ILs depend on nature both ILs, biophysical mechanism governing protein–IL interaction, which determines stability or activity an enzyme these nonconventional media, yet be understood clearly. Herein, we study effect two ammonium triethylammonium dihydrogen...

The dynein family of microtubule minus-end-directed motor proteins drives diverse functions in eukaryotic cells, including cell division, intracellular transport, and flagellar beating. Motor protein processivity, which characterizes how far a walks before detaching from its filament, depends on the interaction between microtubule-binding domain (MTBD) microtubule. Dynein's MTBD switches high- low-binding affinity states as it steps. Significant structural functional data show that specific...

Out-of-equilibrium processes are ubiquitous across living organisms and all structural hierarchies of life. At the molecular scale, out-of-equilibrium (for example, enzyme catalysis, gene regulation, motor protein functions) cause biological macromolecules to sample an ensemble conformations over a wide range time scales. Quantifying conceptualizing structure–dynamics function relationship is challenging because continuously evolving multidimensional energy landscapes necessary describe...

Abstract The recent COVID pandemic has shown major impact on public health and economic crisis. Despite the development of many vaccines drugs against severe acute respiratory syndrome (SARS) coronavirus 2, still persists. continued spread virus is largely driven by emergence viral variants such as α, β, γ, delta, epsilon spike, omicron its subvariants (BA.1,2,3) which can evade current through mutations in spike protein. [1] For instance, to modifications at different (D405N, K417N, S477N,...