A5056896634- Melanoma and MAPK Pathways
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Angiogenesis and VEGF in Cancer
- Cancer Mechanisms and Therapy
- Biomedical Ethics and Regulation
- Cellular Mechanics and Interactions
- Computational Drug Discovery Methods
- Cell Adhesion Molecules Research
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- Extracellular vesicles in disease
- Cutaneous Melanoma Detection and Management
- Kruppel-like factors research
- Wnt/β-catenin signaling in development and cancer
- Inflammatory mediators and NSAID effects
- Sesquiterpenes and Asteraceae Studies
- PI3K/AKT/mTOR signaling in cancer
- Phytochemical compounds biological activities
- Colorectal Cancer Surgical Treatments
- Caveolin-1 and cellular processes
- CRISPR and Genetic Engineering
- Genetic factors in colorectal cancer
- Lymphatic System and Diseases
- Tissue Engineering and Regenerative Medicine
Medical University of Vienna
2015-2024
Medical University of Sofia
2023
Institute of Clinical Research
2015
Many cell lines derived from solid cancers can form spheroids, which recapitulate tumor clusters and are more representative of the in vivo situation than 2D cultures. During spheroid formation, a small proportion variety different colon cancer did not integrate into sphere lost cell-cell adhesion properties. An enrichment protocol was developed to augment these cells 100% purity. The basis for separation spheroids non-spheroid forming (NSF) is simple gravity-sedimentation. This gives rise...
Invasive colorectal cancer is associated with poor prognosis requiring treatment systemic chemotherapies usually including 5-fluorouracil. A consequence of prolonged the acquisition resistance eventually resulting in recurrence highly metastatic cells. To address relationship between drug and increased lymphatic potential, we used a 3D co-culture model colon tumour cell spheroids parent CCL227 cells subclones gradually increasing against From each investigated line, homogeneous were...
Retraction of mesenchymal stromal cells supports the invasion colorectal cancer (CRC) into adjacent compartment. CRC-secreted 12(S)-HETE enhances retraction cancer-associated fibroblasts (CAFs) and therefore, may enforce invasivity CRC. Understanding mechanisms metastatic CRC is crucial for successful intervention. Therefore, we studied pro-invasive contributions in physiologically relevant three-dimensional vitro assays consisting spheroids, CAFs, extracellular matrix endothelial cells, as...
Targeted therapies against mutant BRAF are effectively used in combination with MEK inhibitors (MEKi) to treat advanced melanoma. However, treatment success is affected by resistance and adverse events (AEs). Approved (BRAFi) show high levels of target promiscuity, which can contribute these effects. The blood vessel lining direct contact plasma concentrations BRAFi, but effects the this cell type unknown. Hence, we aimed characterize responses approved BRAFi for melanoma vascular...
RELA, RELB, CREL, NFKB1 and NFKB2, the upstream regulators NEMO NIK were knocked-down in lymph endothelial cells (LECs) MDA-MB231 breast cancer spheroids to study contribution of NF-κB vascular barrier breaching. Suppression inhibited "circular chemo-repellent induced defects" (CCIDs), which form when cross lymphatic vasculature, by ~20-30%. NFKB2 CCIDs only ~10-15%. In RELA constituted MMP1 expression, caused activation PAR1 adjacent LECs. The knock-down pharmacological inhibition LECs CCID...
Melanoma cells can switch between distinct gene expression profiles, resulting in proliferative or invasive phenotypes. Signaling pathways involved this were analyzed by profiling of a cohort 22 patient-derived melanoma cell lines. CDH1 negativity was identified as surrogate marker for the phenotype. could be turned on and off modulating activity p38 its downstream target MK2, suggesting that pathway controls progression. Mechanistically, MK2 inhibition prevented melanoma-induced vascular...
ABSTRACT Dysfunction of vascular barriers is a critical step in inflammatory diseases. Endothelial tight junctions (TJs) control barrier function, and the cytoplasmic adaptor protein cingulin connects TJs to signalling pathways. However, local events at during inflammation are largely unknown. In this study, we investigate response TJ its interaction with Rho guanine nucleotide exchange factor H1 (GEF-H1, also known as ARHGEF2) upon disruption find new approach counteract leak. Based on...
Lymph node metastasis of breast cancer is a clinical marker poor prognosis. Yet, there exist no therapies targeting mechanisms intravasation into lymphatics. Herein we report on an effect the antidyslipidemic drug fenofibrate with vasoprotective activity, which attenuates in vitro, and describe potential mechanisms. To measure 3-dimensional co-culture model MDA-MB231 MCF-7 spheroids were placed immortalised lymphendothelial cell (LEC) monolayers. This provokes formation circular...
Abstract Background: The use of peripheral blood mononuclear cells (PBMCs) and urine-derived epithelial for reprogramming towards induced pluripotent stem (iPSCs) has shown to be highly effective. Due their easy accessibility, these cell sources hold promising potential non-invasive repetitive isolation from patients. This study aims conduct a comparative analysis the phenotype, differentiation efficacy, functional properties iPSCs derived PBMCs urine endothelial (EC) vascular smooth muscle...
Background and Objectives: Peripheral blood mononuclear cells (PBMCs) urine‐derived epithelial have both emerged as valuable sources for induced pluripotent stem cell (iPSC) generation, each presenting unique advantages in terms of accessibility reprograming efficiency. This study aimed to assess compare the potential PBMC‐derived iPSCs (PiPSCs) (UiPSCs) generating functional endothelial (ECs) vascular smooth muscle (VSMCs), which are critical tissue engineering disease modeling. Phenotypic...
Abstract Targeted therapies against mutant BRAF are effectively used in combination with MEK inhibitors (MEKi) to treat advanced melanoma. However, treatment success is affected by resistance and adverse events (AEs). Approved (BRAFi) show high levels of target promiscuity, which can contribute these effects. Blood vessels direct contact plasma concentrations BRAFi, but effects the this cell type unknown. Hence, we aimed characterize responses approved BRAFi for melanoma vascular...