Objective Non-bacterial thrombotic endocarditis (NBTE) is a syndrome characterised by cardiac valve vegetations and/or thickening due to non-infective mechanisms. Nowadays, premortem diagnosis of NBTE possible based on echocardiographic findings. Therefore, better characterise this disease, we performed contemporary review the epidemiology, demographics, and clinical outcomes these patients. Methods Adults with seen within Mayo Clinic Enterprise from December 2014 2021 were included. was...

Recent advances in shotgun metagenomic sequencing (sMGS) for detecting microbial cell-free DNA (mcfDNA) peripheral blood have shown promise across various patient populations. This study evaluates the application of sMGS diagnosing osteoarticular infections (OAIs), a condition with significant diagnostic challenges. We conducted retrospective analysis on 73 patients suspected OAIs at Mayo Clinic from 2019 to 2023, incorporating mcfDNA (Karius test [KT]) into their evaluation. categorized...

Abstract Background The introduction of shotgun metagenomic sequencing (sMGS) methods that identify the presence microbial cell-free DNA (mcfDNA) in peripheral blood have shown promising results various populations patients. There is a pressing need to evaluate its application for osteoarticular infections (OAIs) real-world setting. Baseline characteristics patients with suspected osteo-articular infections, including demographics, clinical characteristics, diagnosis, and KT results. Methods...

Abstract. Background: Elbow native joint septic arthritis (NJSA) is a rare condition, constituting 6 %–9 % of all cases. It associated with elevated mortality and morbidity. This study aims to clarify the characteristics, management, outcomes elbow NJSA. Methods: We retrospectively analyzed adults diagnosed NJSA who underwent surgical intervention at Mayo Clinic facilities from January 2012 December 2021. Diagnosis relied on clinical presentation, synovial fluid white blood cell (WBC) count,...

Native joint septic arthritis (NJSA) is definitively diagnosed by a positive Gram stain or culture, along with supportive clinical findings. Preoperative antibiotics are known to alter synovial fluid cell count, stain, and culture results typically postponed until after arthrocentesis optimize diagnostic accuracy. However, data on the impact of preoperative operative yield for NJSA diagnosis limited.

Identifying and treating patients with acute Q fever who are at an increased risk of progressing to persistent disease is crucial for preventing future complications. In this study, we share our decade-long clinical experience fever, highlighting the challenges that clinicians encounter from making initial diagnosis performing stratification determining appropriate prophylaxis regimen duration.

Mycobacterium haemophilum is a nontuberculous mycobacterium with fastidious in vitro growth requirements and an increasingly reported cause of extrapulmonary disease. Timely diagnosis management M. infections the immune reconstitution inflammatory syndromes (IRIS) observed subset patients during treatment remain challenging.We conducted retrospective chart review between January 1, 2010, 2022 identified 26 diagnosed infection at our institution. We describe their clinical presentation,...

Abstract. Introduction: The absence of a standardized postoperative antibiotic treatment approach for patients with surgically treated septic bursitis results in disparate practices. Methods: We retrospectively reviewed charts adult olecranon at Mayo Clinic sites between 1 January 2000 and 20 August 2022, focusing on their clinical presentation, diagnostics, management, use, outcomes. Results: A total 91 were identified during the study period. Staphylococcus aureus was most common pathogen...

Abstract We examined the effect of preoperative antibiotic exposure and duration on synovial fluid samples from patients with native joint septic arthritis hip/knee. While before diagnostic arthrocentesis did not affect parameters, increased was associated a decreased total nucleated cell count, underscoring complex effects parameters.

ABSTRACT A total of 1,925 Corynebacterium isolates were tested for antimicrobial susceptibility at the Mayo Clinic Microbiology laboratory (Rochester, Minnesota) from January 2012 to March 2023, with C. striatum (35.6%) and amycolatum (24.4%) identified as predominant species. Species known potentially carry diphtheria toxin excluded. Common sources isolation included skin soft tissue (56.8%), bone and/or native joint synovial fluid (14.2%), urine (13.1%), sputum (6.1%), blood (5.9%). For...

Abstract Background Non-diphtheriae Corynebacterium spp. are important human pathogens responsible for a broad spectrum of clinical syndromes. Data on species-specific susceptibility patterns is limited. In this study we aim to these over time and summarize the resistance profile that could be used by clinicians guide therapeutic decisions. Methods We conducted retrospective review all isolates were submitted antimicrobial testing (AST) between January-2012 February 2023 our reference lab....

Abstract Background Native joint septic arthritis (NJSA) is diagnosed by synovial fluid analysis to evaluate for evidence of infection but may be definitively established a positive gram stain or culture. Antibiotics given prior sampling have been described alter cell count, stain, and culture results are conventionally deferred until after arthrocentesis optimize diagnostic yield. However, there limited data on the impact preoperative antibiotic therapy operative yield in diagnosis NJSA....

Abstract Background Diagnosing acute Q fever is challenging partly due to the non-specific and self-limited course of illness in majority cases. It important identify treat patients who are at increased risk for progression persistent disease prevent future complications. We describe demographics, clinical presentation, treatment diagnosed with our institution. Methods identified all between December 2019 March 2022. Patients 18 years or older had a anti-phase II IgM ≥ 1:50 IgG 200 were...