A5006070471- Pain Mechanisms and Treatments
- Vestibular and auditory disorders
- Neurobiology and Insect Physiology Research
- Hereditary Neurological Disorders
- Ion channel regulation and function
- Hearing, Cochlea, Tinnitus, Genetics
- Axon Guidance and Neuronal Signaling
- Muscle Physiology and Disorders
- RNA Research and Splicing
- Genetics and Neurodevelopmental Disorders
- Developmental Biology and Gene Regulation
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience of respiration and sleep
- Connexins and lens biology
- Fungal and yeast genetics research
- Adipose Tissue and Metabolism
- Epigenetics and DNA Methylation
- Neuroscience and Neuropharmacology Research
- Pluripotent Stem Cells Research
- Plant Reproductive Biology
- Zebrafish Biomedical Research Applications
- Genomics and Chromatin Dynamics
- Neuroscience and Neural Engineering
- Pain Management and Placebo Effect
- Nerve injury and regeneration
The University of Texas Southwestern Medical Center
2009-2024
Southwestern Medical Center
2009-2024
Small Business Administration
2020
Duke University
2018
Duke University Hospital
2016
Duke Medical Center
2016
University of California, San Francisco
2005
Howard Hughes Medical Institute
2005
Johns Hopkins University
2003
Chinese University of Hong Kong
2003
The human genome is far smaller than originally estimated, and one explanation that alternative splicing creates greater proteomic complexity a simple count of open reading frames would suggest. p53 homologue p63, for example, tetrameric transcription factor implicated in epithelial development expressed as at least six isoforms with widely differing transactivation potential. In particular, p63alpha contain 27-kDa C-terminal region drastically reduces their activity clear biological...
Neurogenesis requires neural progenitor cell (NPC) proliferation, neuronal migration, and differentiation. During embryonic development, neurons are generated in specific areas of the developing neuroepithelium migrate to their appropriate positions. In adult brain, neurogenesis continues subgranular zone (SGZ) hippocampal dentate gyrus subventricular (SVZ) lateral ventricle. Although is fundamental brain development function, our understanding molecular mechanisms that regulate still...
Abstract Somatic sensation is defined by the existence of a diversity primary sensory neurons with unique biological features and response profiles to external internal stimuli. However, there no coherent picture about how this cell states transcriptionally generated. Here, we use deep single analysis resolve fate splits molecular biasing processes during neurogenesis in mice. Our results identify complex series successive specific transcriptional changes post-mitotic that delineate...
The sensation of pain is essential for the preservation functional integrity body. However, key molecular regulators necessary initiation development pain-sensing neurons have remained largely unknown. Here, we report that, in mice, inactivation transcriptional regulator PRDM12, which perception humans, results a complete absence nociceptive lineage, while proprioceptive and touch-sensitive remain. Mechanistically, our data reveal that PRDM12 required neurogenesis activation cascade...
Neural basic helix-loop-helix (bHLH) transcription factors are crucial in regulating the differentiation and neuronal subtype specification of neurons. Precisely how these direct such processes is largely unknown due to lack bona fide targets <i>in vivo</i>. Genetic evidence suggests that bHLH have shared their common role, but unique with respect distinct roles specification. However, whether subtype-specific exist remains an unsolved question. To address this question, we focused on Atoh1...
Proprioception, the sense of limb and body position, is essential for generating proper movement. Unconscious proprioceptive information travels through cerebellar-projecting neurons in spinal cord medulla. The progenitor domain defined by basic-helix-loop-helix (bHLH) transcription factor, ATOH1, has been implicated forming these neurons; however, their precise contribution to tracts motor behavior unknown. Significantly, we demonstrate that Atoh1-lineage reside outside Clarke's column...
Tip growth in fungi involves highly polarized secretion and modification of the cell wall at growing tip. The genetic requirements for initiating are perhaps best understood model budding yeast Saccharomyces cerevisiae. Once is committed to enter cycle by activation G1 cyclin/cyclin-dependent kinase (CDK) complexes, polarity regulator Cdc42 becomes concentrated presumptive bud site, actin cables oriented toward that septin filaments assemble into a ring around site. Several minutes later,...
Prdm12 is a key transcription factor in nociceptor neurogenesis. Mutations of cause congenital insensitivity to pain (CIP) from failure development. However, precisely how deletion during development or adulthood affects nociception unknown. Here, we employ tissue- and temporal-specific knockout mouse models test the function adulthood. We find that constitutive loss causes deficiencies proliferation sensory also demonstrate conditional dorsal root ganglia (DRGs) embryogenesis defects...
Proprioception, the sense of limb and body position, generates a map that is essential for proper motor control, yet we know little about precisely how neurons in proprioceptive pathways are wired. Defining anatomy secondary spinal cord integrate relay potentially cutaneous information from periphery to cerebellum fundamental understanding circuits function. Here, define unique anatomic trajectories long-range direct indirect spinocerebellar as well local intersegmental using genetic tools...
Despite their opposing actions on food intake, POMC and NPY/AgRP neurons in the arcuate nucleus of hypothalamus (ARH) are derived from same progenitors that give rise to ARH neurons. However, mechanism whereby common neuronal precursors subsequently adopt either anorexigenic (POMC) or orexigenic (NPY/AgRP) identity remains elusive. We hypothesize cell fates specified maintained by distinct intrinsic factors. In search them, we profiled transcriptomes developing mice. Moreover,...
<h3>Abstract</h3> Motor neurons (MNs) innervating the digit muscles of intrinsic hand (IH) and foot (IF) control fine motor movements. The ability to reproducibly label specifically IH IF MNs in mice would be a beneficial tool for studies focused on control. To this end, we find that CRE knock-in mouse line <i>Atoh1</i>, developmentally expressed basic helix-loop-helix (bHLH) transcription factor, reliably expresses CRE-dependent reporter genes ∼60% MNs. We determine expression is ectopic...
This review highlights similarities in human, rodent and avian eye development, focusing on morphogenesis, proliferative activity, apoptosis mechanistic aspects during the development of retina, iris cornea. Retinal morphogenesis encompasses four stages: (1) appearance ganglion cell layer, (2) formation outer plexiform layer separating inner nuclear layers, (3) immature photoreceptor segments (4) segments. The first two stages occur very early i.e. within 10 days incubation chickens,...