Lizard tail autotomy is an antipredator strategy consisting of sturdy attachment at regular times but quick detachment during need. We propose a biomimetic fracture model lizard using multiscale hierarchical structures. The structures consist uniformly distributed micropillars with nanoporous tops, which recapitulate the high-density mushroom-shaped microstructures found on tail's muscle plane. experiments showed adhesion enhancement when combining interfacial surfaces flexible in tensile...

It is extremely advantageous to detect nucleic acid levels in the early phases of disease management; such detection facilitates timely treatment, and it can prevent altogether certain cancers infectious diseases. A simple, rapid, versatile platform without enzymatic amplification for both short long sequences would be highly desirable this regard. Our study addresses need by introducing IMACC, an ICP-based Microfluidic Accelerator Combined with CRISPR, amplification-free detection. exploits...

Exosomes have gained immense importance since their proteomic and genetic contents could potentially be used for disease diagnostics, monitoring of cancer progression, metastasis, drug efficacy. However, establishing the clinical utility exosomes has been restricted due to small sizes high sample loss from extensive preparation. Sample is particularly critical body fluids limited in volume difficult access, e.g., cerebrospinal fluid. We present a microfluidic technique that locally enhances...

Flow-based microfluidic biochips (FMBs) have seen rapid commercialization and deployment in recent years for point-of-care clinical diagnostics. However, the outsourcing of FMB design manufacturing makes them susceptible to malicious physical level intellectual property (IP)-theft attacks. This work demonstrates first structure-based (SB) attack on representative commercial FMBs. The SB attacks maliciously decrease heights reaction chambers produce false-negative results. We validate this...

A microfluidic force assay chip was used to quantify the relative changes in thrashing of<italic>C. elegans</italic>upon exposure various external stimuli.

Researchers develop bioassays following rigorous experimentation in the lab that involves considerable fiscal and highly-skilled-person-hour investment. Previous work shows a bioassay implementation can be reverse-engineered by using images or video control signals of biochip. Hence, techniques must devised to protect intellectual property (IP) rights developer. This study is first step this direction it makes contributions: (1) introduces use dummy valve as security primitive obfuscate...

Over the past few years, detection of biological analytes has intensively been studied to understand biodiagnostics various diseases [1]. Some that have used as biomarkers for specific include cell-free nucleic acids, (for example, DNA), messenger RNA (mRNA) and microRNA (miRNA) [2]-[5], circulating tumor-specific acids [circulating tumor DNA (ctDNA) ctRNA] [6]-[8], exosomes [9], [10], cells [11]. These biomolecules are known be effective indicators can diagnose presence a potential disease...

Biological soft interfaces often exhibit complex microscale interlocking geometries to ensure sturdy and flexible connections. If needed, the can rapidly be released on demand leading an abrupt decrease of interfacial adhesion. Here, inspired by lizard tail autotomy where such apparently tunable fracture behavior observed, we hypothesized mechanism between body based muscle-actuated mushroom-shaped microinterlocks along planes. To mimic tail, developed a bilayer patch that consisted dense...

We examine the underlying fracture mechanics of human skin dermal-epidermal layer's microinterlocks using a physics-based cohesive zone finite-element model. Using microfabrication techniques, we fabricated highly dense arrays spherical microstructures radius $\ensuremath{\approx}50\phantom{\rule{0.16em}{0ex}}\ensuremath{\mu}\mathrm{m}$ without and with undercuts, which occur in an open cavity whose centroid lies below microstructure surface to create polydimethylsiloxane layers. From...

In this study, deoxyribonucleic acid (DNA) hybridization was demonstrated using a suspended silicon photonics micro-ring resonator with 90 nm-thick slab and morpholino as the capture probe. Complementary DNA of various concentrations were tested achieving surface sensitivity 2.12 nm/nM, detection limit 250 pM, an intrinsic 36.9 pM. A bulk 98 nm/RIU <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex-math notation="LaTeX">$1.03\times...

Microfluidic devices offer new technical possibilities for a precise manipulation of Caenorhabditis elegans due to the comparable length scale. C. is small, free-living nematode worm that popular model system genetic, genomic, and high-throughput experimental studies animal development neurobiology. In this paper, we demonstrate microfluidic in polydimethylsiloxane (PDMS) dispensing single into 96-well plate. It consists two PDMS layers, flow control layer. Using five pneumatic valves layer,...

The elasticity of white blood cells (WBCs) provides valuable insight into the condition themselves, presence some diseases, as well immune system activity. In this work, we describe a novel process refined control WBCs’ through combined use gold nanoparticles (AuNPs) and microelectrode array device. capture controlled deformation enriched in vitro are demonstrated quantified. Gold enhance effect electrically induced make DEP-related processes more controllable.

Microfluidic biochips enable low-cost automation of biochemical protocols with numerous applications to medical diagnostics, forensics, molecular biology, and drug design. An important component protocol design is sample preparation, which involves dilution or mixing two more fluids in a desired ratio concentration factors (CF). Existing continuous-flow microfluidic deploy either free-flowing networks where only single layer flow-channels used devoid any control valves, valve-based...

Small multicellular organisms, such as C. elegans and zebrafish larvae, are essential models in biological research, but their 3D manipulation poses challenges due to small size. Manual positioning is labor-intensive imprecise. To address this, we developed a soft PDMS microtube that can be gently stretched released, immobilizing organisms without anesthetization damage. This tube enables easy rotation adjustment of orientation, facilitating comprehensive imaging. Functionality testing...

Flow-based microfluidic biochips (FMBs) have microvalves as key components. The physical characteristics of the vary instance-to-instance due to inherent variability numerous fabrication parameters. In this work, we leverage unclonable, unpredictable instance-specific behavior and propose physically unclonable functions (PUFs) for FMBs, namely Biochip-PUFs (Bio-PUFs in short). We utilize microvalve membrane deflection response associated with actuation pressure challenge be our Bio-PUF...

Organoids have gained significant interests in the recent years to recapitulate organ development and disease progression in-vitro for personalized medicine. We propose a microfluidic chip design grow organoids spheroids of more uniform size shape with minimal intervention. The hybrid allows open close culture wells using reversibly bonded cover sheet. By combining advantages similar conventional microplate sealed channel chips, cells can directly be pipetted into individual continuously...

Abstract The elasticity of white blood cells (WBCs) provides valuable insight into the condition themselves, presence some diseases, as well immune system activity. In this work, we describe a novel process refined control WBCs’ through combined use gold nanoparticles (AuNPs) and microelectrode array device. capture controlled deformation enriched in vitro are demonstrated quantified. Gold enhance effect electrically induced make DEP related processes more controllable.