A5040817129- Prostate Cancer Treatment and Research
- Cancer Genomics and Diagnostics
- Enzyme function and inhibition
- Prostate Cancer Diagnosis and Treatment
- Bladder and Urothelial Cancer Treatments
- Phonetics and Phonology Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Molecular Biology Techniques and Applications
- Ion Transport and Channel Regulation
- Immune cells in cancer
- Cholinesterase and Neurodegenerative Diseases
- Radiopharmaceutical Chemistry and Applications
- Chemical Reactions and Mechanisms
- Immunotherapy and Immune Responses
- Cancer, Lipids, and Metabolism
- Synthesis and Catalytic Reactions
- Glutathione Transferases and Polymorphisms
- Diamond and Carbon-based Materials Research
- Plant Surface Properties and Treatments
- Cancer-related molecular mechanisms research
- Neutropenia and Cancer Infections
- Polyamine Metabolism and Applications
- Renal cell carcinoma treatment
- Speech and dialogue systems
- Cancer, Hypoxia, and Metabolism
Tampere University
2011-2025
Tampere University Hospital
2006-2024
University of Jyväskylä
2022
Fimlab (Finland)
2015-2020
Prostate Cancer Research
2017
Cancer Research Center
2017
Tampere University of Applied Sciences
2011
A significant subset of prostate cancer (PC) patients with a castration-resistant form the disease (CRPC) show primary resistance to androgen receptor (AR)-targeting drugs developed against CRPC. As one explanation could be expression constitutively active splice variants (AR-Vs), our current objectives were study AR-Vs and other AR aberrations better understand emergence CRPC.We analysed specimens from different stages by next-generation sequencing immunohistochemistry.AR mutations copy...
Epithelial ovarian cancer (EOC) is one of the leading causes cancer-related death in women worldwide, and characterized by a high rate recurrence after surgery chemotherapy. We sought to implement circulating tumor DNA (ctDNA)–based blood test for more accurate post-operative surveillance this disease. analyzed 264 plasma samples collected between June 2016 September 2021 from 63 EOC patients using tumor-guided cell-free analysis detect residual disease treatment. Assay specificity was...
Abstract Prostate cancer treatment resistance is a significant challenge facing the field. Genomic and transcriptomic profiling have partially elucidated mechanisms through which cells escape treatment, but their relation toward tumor microenvironment (TME) remains elusive. Here we present comprehensive landscape of prostate TME at multiple points in standard timeline employing single-cell RNA-sequencing spatial transcriptomics data from 120 patients. We identify club-like as key epithelial...
We report the first combined analysis of whole-genome sequence, detailed clinical history, and transcriptome sequence multiple prostate cancer metastases in a single patient (A21). Whole-genome was obtained from nine anatomically separate metastases, targeted DNA sequencing performed cancerous noncancerous foci within primary tumor specimen removed 5 yr before death. Transcriptome revealed increased expression androgen receptor (AR)-regulated genes liver that harbored an AR p.L702H mutation,...
Abstract Introduction: The prostate cancer tumor microenvironment (TME) contains various cell type identities. We recently described club-like epithelial cells as a constitutive part of the TME present in tissue regions with increased myeloid-derived suppressor infiltration. origin, exact function, and identity remain unresolved. Methods: Here we used bulk, single-cell, spatial transcriptomics to characterize their relation towards androgen deprivation therapy. additionally analyzed...
Abstract Background Of the thirteen active carbonic anhydrase (CA) isozymes, CA IX and XII have been linked to carcinogenesis. It has suggested that these membrane-bound CAs participate in cancer cell invasion, which is facilitated by an acidic tumor environment. Since migration a characteristic feature of embryonic development, we set out explore whether isozymes are expressed mouse embryos different ages. The studies were focused on organogenesis stage. Results Immunohistochemistry...
Our previous microarray study showed that the non-specific cytotoxic cell receptor protein 1 (Nccrp1) transcript is significantly upregulated in gastric mucosa of carbonic anhydrase IX (CA IX)-deficient (Car9−/−) mice. In this paper, we aimed to characterize human NCCRP1 and elucidate its relationship CA IX. Recombinant was expressed Escherichia coli, a novel polyclonal antiserum raised against purified full-length protein. Immunocytochemistry intracellularly, even though it has previously...
Peroxiredoxins (Prxs) have recently been suggested to a role in tumorigenesis. We studied the expression of Prx I-VI and their relationship patient survival 383 grade II-IV diffuse astrocytic brain tumors. I positivity was found 68%, II 84%, III 90%, IV 5%, V 4% VI 47% decreased significantly with increasing malignancy (p < 0.001 p 0.001). Patients or positive tumors were younger than average age all patients = 0.014 0.005). A lower proliferation rate associated 0.019 0.033), apoptotic...
Advances in prostate cancer biology and diagnostics are dependent upon high-fidelity integration of clinical, histomorphologic, molecular phenotypic findings. In this study, we compared fresh frozen, formalin-fixed paraffin-embedded (FFPE), PAXgene-fixed (PFPE) tissue preparation methods radical prostatectomy from 36 patients performed a preliminary test feasibility using PFPE routine surgical pathology diagnostic assessment. addition to comparing histology, immunohistochemistry, general...
Carbonic anhydrases (CAs) are a family of enzymes that regulate pH homeostasis in various tissues. CA IX is an exceptional member this because addition to the basic function, it has been implicated several other physiological and pathological processes. Functions suggested for include roles cell adhesion malignant invasion. In addition, likely regulates proliferation differentiation, which was demonstrated Car9-/- mice. These mice had gastric pit hyperplasia depletion chief cells; however,...
While utterance fluency measures are often studied in relation to perceived L2 and proficiency, the effect of creaky voice remains ignored.However, is frequent a number languages, including Finnish, where it serves as cue for phrase-boundaries turn-taking.In this study we investigate roles predicting proficiency ratings spontaneous Finnish (F2) speech.In so doing, 16 expert raters participated assessing narrative speech samples from 160 learners Finnish.The on was using linear regression...
AIM: Carbonic anhydrase Ⅸ (CA9) and carbonic Ⅻ (CA12) are cancer-associated enzymes that strongly up-regulated by hypoxia via hypoxia-inducible factor HIF-1, but whether these isozymes regulated other mechanisms is not well understood. In the present study, we investigated effects of certain hormones growth factors on levels CA9 CA12 mRNA expression in human cancer cell lines.METHODS: Seven lines were selected for study. The cells treated with several 24 h. Changes transcripts detected using...
Abstract Prostate cancer treatment resistance is a significant challenge facing the field. Genomic and transcriptomic profiling have partially elucidated mechanisms through which cells escape treatment, but their relation toward tumor microenvironment (TME) remains elusive. Here we present comprehensive landscape of prostate TME at multiple points in standard timeline employing single-cell RNA-sequencing spatial transcriptomics data from 110 patients. We identify club-like as key epithelial...
Abstract Background: The detection of cancer-associated DNA in urine samples has significant potential urothelial cancer (UC) and surveillance. Every year millions patients with urinary symptoms undergo cystoscopy, an examination the bladder using a flexible scope. Only 10% these invasive examinations result diagnosis. Cystoscopy is also used post-operative surveillance, many UC requiring life-long cystoscopies. Methods: We developed high-volume collection kit laboratory platform...
You have accessJournal of UrologyBladder Cancer: Epidemiology & Evaluation II (MP35)1 May 2024MP35-05 DEVELOPING NON-INVASIVE AND COST-EFFECTIVE METHODS FOR UROTHELIAL CANCER DETECTION SURVEILLANCE USING A URINE DNA PANEL (UROSCOUT ASSAY) Jussi Nikkola, Thea Veitonmäki, Lauri Ryyppö, Juuso Vuorinen, Heini Kallio, Hanna Selin, Pyry Jämsä, Antti Kaipia, Tarmo Pekkarinen, Jonne Åkerla, Matti Nykter, and Annala NikkolaJussi Nikkola , VeitonmäkiThea Veitonmäki RyyppöLauri Ryyppö VuorinenJuuso...
Fluency is a commonly used descriptor of second language (L2) speaking skills.Unplanned and too frequent pauses, hesitations, repetitions disrupt the flow speech can cause temporal irregularities at all levels hierarchy, from syllable rate to phrasing.However, most studies that attempt quantify fluency disregard pause locations.The current study investigates, whether which locations affect perceived proficiency in L2 Finnish.Several parameters were computed spontaneous Finnish samples.Pause...
Abstract Prostate cancer (PC) is the most commonly diagnosed male both in United States and Europe. Approximately 20-25% of cases will develop metastatic disease, which eventually progresses to lethal castration-resistant form disease (CRPC). Even though exact mechanism by CRPC develops remains be fully understood, several mechanisms castration resistance have been identified. Importantly, androgen signaling active even stage. This has led clinical development second-generation AR-targeting...