A5078237945- Insect Resistance and Genetics
- Insect Pest Control Strategies
- Enzyme Production and Characterization
- Biochemical and Structural Characterization
- Protein Hydrolysis and Bioactive Peptides
- Agricultural pest management studies
- Marine Sponges and Natural Products
- Proteoglycans and glycosaminoglycans research
- Carbohydrate Chemistry and Synthesis
- Invertebrate Immune Response Mechanisms
- Essential Oils and Antimicrobial Activity
- Regulation of Appetite and Obesity
- Phytase and its Applications
- Glycosylation and Glycoproteins Research
- Seaweed-derived Bioactive Compounds
- Biochemical Analysis and Sensing Techniques
- Proteins in Food Systems
- Insect Utilization and Effects
- Pharmacological Effects of Natural Compounds
- Biofuel production and bioconversion
- Aquaculture Nutrition and Growth
- Microbial Metabolites in Food Biotechnology
- Protease and Inhibitor Mechanisms
- Bioactive Compounds and Antitumor Agents
- Adipokines, Inflammation, and Metabolic Diseases
Universidade Regional do Cariri
2025
Universidade Federal do Rio Grande do Norte
2015-2025
Universidade Federal de Pernambuco
2019-2025
Universidade Federal do Rio Grande
2007-2022
Instituto Federal de Educação, Ciência e Tecnologia do Ceará
2019-2021
Instituto Federal do Rio Grande do Norte
2021
Universidade Estadual Paulista (Unesp)
2011
Brazilian Biosciences National Laboratory
2008-2009
Universidade Federal de Minas Gerais
2007
Universidade Federal do Ceará
2006-2007
Fucan is a term used to denote family of sulfated L-fucose-rich polysaccharides which are present in the extracellular matrix brown seaweed and egg jelly coat sea urchins. Plant fucans have several biological activities, including anticoagulant antithrombotic, related structural chemical composition polysaccharides. We extracted from Dictyota menstrualis by proteolytic digestion, followed separation into 5 fractions sequential acetone precipitation. Gel electrophoresis using 0.05 M...
This study set out to evaluate how effective the unexplored macrofungi Langermannia bicolor is in providing a biomolecular repertoire capable of optimizing action antibacterials available on market. Extract and fractions (obtained by fractionation with acetone) L. were quantified for presence proteases hemagglutinin, composition carbohydrates, proteins, phenolic flavonoid compounds; then, they tested against multidrug-resistant gram-positive gram-negative strains combined conventional...
Cancer represents a set of more than 100 diseases, including malignant tumors from different locations. Strategies inducing differentiation have had limited success in the treatment established cancers. Marine sponges are biological reservoir bioactive molecules, especially lectins. Several animal and plant lectins were purified with antitumor activity, mitogenic, anti-inflammatory antiviral, but there few reports literature describing mechanism action marine to induce apoptosis human tumor...
Inhibitors of peptidases isolated from leguminous seeds have been studied for their pharmacological properties. The present study focused on purification, biochemical characterization and anti-inflammatory anticoagulant evaluation a novel Kunitz trypsin inhibitor Erythrina velutina (EvTI). Trypsin inhibitors were purified by ammonium sulfate (30-60%), fractionation followed Trypsin-Sepharose affinity chromatography reversed-phase high performance liquid chromatography. showed molecular mass...
Soybean is one of the most common grain crops worldwide, representing an important protein and oil source. Although genetic variability in chemical composition grains seen soybean, mean levels proteins have remained stagnant or, some cases, decreased over time, arousing concern agricultural industry. Furthermore, environmental conditions influence grains. Thus, present study evaluated effect water deficit (WD) induced at vegetative period (vegetative stress (VS)) reproductive (reproductive...
OBJECTIVES: Seeds are excellent sources of proteinase inhibitors, some which may have satietogenic and slimming actions.We evaluated the effect a trypsin inhibitor from Tamarindus indica L. seeds on weight gain, food consumption cholecystokinin levels in Wistar rats.METHODS: A was isolated using ammonium sulfate (30-60%) following precipitation with acetone further Trypsin-Sepharose affinity chromatography.Analyses were conducted to assess vivo digestibility, intake, body evolution...
Crude extract from the sponge Cinachyrella apion showed cross-reactivity with polyclonal antibody IgG anti-CvL (Cliona varians lectin) and also a strong haemagglutinating activity towards human erythrocytes of all ABO groups. Thus, it was submitted to acetone fractionation, anti-deglycosylated CvL Sepharose affinity chromatography, Fast Protein Liquid Chromatography (FPLC-AKTA Purifier) gel filtration on Superose 6 10/300 column purify novel lectin. C. lectin (CaL) agglutinated types...
Trypsin inhibitors are studied in a variety of models for their anti-obesity and anti-inflammatory bioactive properties. Our group has previously demonstrated the satietogenic effect tamarind seed trypsin (TTI) eutrophic mouse effects other inhibitors. In this study, we evaluated TTI upon satiety, biochemical inflammatory parameters an experimental model metabolic syndrome (MetS). Three groups n = 5 male Wistar rats with obesity-based MetS received 10 days one following: (1) Cafeteria diet;...
A trypsin inhibitor isolated from tamarind seed (TTI) has satietogenic effects in animals, increasing the cholecystokinin (CCK) eutrophy and reducing leptin obesity. We purified TTI (pTTI), characterised, observed its effect upon CCK obese Wistar rats. By HPLC, after amplification of resolution, two protein fractions were observed: Fr1 Fr2, with average mass [M + 14H]+ = 19,594,690 Da 13H]+ 19,578,266 Da, respectively. The showed 54 53 amino acid residues same sequence. pTTI presented...
Ingestion of peanuts may have a beneficial effect on weight control, possibly due to the satietogenic action trypsin inhibitors. The aim this study was isolate new inhibitor in typical Brazilian peanut sweet (paçoca) and evaluate its biochemical parameters, gain food intake male Wistar rats. paçoca (AHTI) isolated. Experimental diets were prepared with AIN-93G supplemented AHTI. Animals had their monitored. anesthetized, euthanized, bloods collected by cardiac puncture for dosage...
A natural heparin-like compound isolated from the crab Goniopsis cruentata was structurally characterized and its anticoagulant hemorrhagic activities were determined. Enzymatic nuclear magnetic resonance analysis revealed that structure is rich in disulfated disaccharides, possessing significant amounts of 2-O-sulfated-β-D-glucuronic acid units. Furthermore, low trisulfated disaccharide units containing 2-O-sulfated-α-L-iduronic detected, when compared to mammalian heparin. In addition,...
The increasing prevalence of obesity and, consequently, chronic inflammation and its complications has increased the search for new treatment methods. effect purified tamarind seed trypsin inhibitor (TTIp) on metabolic alterations in Wistar rats with dyslipidemia was evaluated. Three groups animals were formed, consuming a high glycemic index load (HGLI) diet, 10 days: Obese/HGLI diet; Obese/standard diet + TTIp (730 μg/kg); one eutrophic group fed standard diet. Rats evaluated daily food...
A trypsin inhibitor, PdKI, was purified from Pithecellobium dumosum seeds by TCA precipitation, trypsin-sepharose chromatography, and reversed-phase-HPLC. PdKI 217.6-fold recovered 4.7%. SDS-PAGE showed that is a single polypeptide chain of 18.9 kDa 19.7 MALDI-TOF. The inhibition on stable in the pH range 2-10 at temperature 50 degrees C. Ki values were 3.56 x 10(-8)and 7.61 10(-7) M with competitive noncompetitive mechanisms for papain, respectively. N-terminal sequence identified members...