A5063194139- Pluripotent Stem Cells Research
- Single-cell and spatial transcriptomics
- Neurobiology and Insect Physiology Research
- RNA Research and Splicing
- Mathematical Biology Tumor Growth
- Congenital heart defects research
- Cell Image Analysis Techniques
Champalimaud Foundation
2023-2024
Development of multicellular organisms is orchestrated by persistent cell-cell communication between neighboring partners. Direct interaction different cell types can induce molecular signals that dictate lineage specification and fate decisions. Current single-cell RNA-seq technology cannot adequately analyze contact-dependent gene expression, mainly due to the loss spatial information. To overcome this obstacle resolve contact-specific expression during embryogenesis, we performed RNA...
Live imaging has been instrumental in understanding cellular dynamics Drosophila tissues, but technical limitations have prevented long-term visualization of cell competition adult brains. Here, we describe a simple ex-vivo protocol that enables extended live brains for up to 32 hours. The method relies on non-supplemented Schneider's medium and hydrophobic interactions maintain brain stability during imaging, eliminating the need complex culture conditions or embedding procedures. We...
Abstract Tissues encompass a quality control mechanism that promotes their optimal state. This mechanism, designated cell competition, is characterised by the elimination of suboptimal yet viable cells when they are near healthier within same tissue compartment. study explores Flower-dependent competition and introduces Ikebana as novel player. The differential expression flower isoforms labels winners or losers, influencing fate in diverse contexts, including eye development, traumatic...
Abstract Competitive interactions between tumor cells and surrounding healthy are constantly present during the progression of a solid tumor, their outcome has been proposed to affect clinical behavior. Previous studies have described various mechanistic molecular aspects that characterize this process, overall indicating cancer behave as supercompetitors, which eliminate neighboring gain vital space for growth infiltration tissue. Nevertheless, there is lack systematic characterization...
Summary Development of multicellular organisms is orchestrated by persistent cell-cell communication between neighboring partners. Direct interaction different cell types can induce molecular signals that dictate lineage specification and fate decisions. Current single RNAseq (scRNAseq) technology cannot adequately analyze contact-dependent gene expression, mainly due to the loss spatial information. To overcome this obstacle resolve contact-specific expression during embryogenesis, we...