A5046991705- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cytokine Signaling Pathways and Interactions
- Machine Learning in Materials Science
- S100 Proteins and Annexins
- Transgenic Plants and Applications
- Influenza Virus Research Studies
- Immunotherapy and Immune Responses
- RNA and protein synthesis mechanisms
- Antimicrobial Peptides and Activities
- Plant-Microbe Interactions and Immunity
- Calcium Carbonate Crystallization and Inhibition
- Immune cells in cancer
- Computational Drug Discovery Methods
- interferon and immune responses
The University of Queensland
2021-2025
MyD88 and MAL are Toll-like receptor (TLR) adaptors that signal to induce pro-inflammatory cytokine production. We previously observed the TIR domain of (MAL
Abstract Toll-like receptor 4 (TLR4) signaling at the plasma membrane and in endosomes results distinct contributions to inflammation host defence. Current understanding indicates that endocytosis of cell surface-activated TLR4 is required enable subsequent from endosomes. Contrary this prevailing model, our data show endosomal not reliant on surface-expressed or ligand-induced endocytosis. Moreover, previously recognized requirements for accessory molecule CD14 are likely attributable...
TLRs engage numerous adaptor proteins and signaling molecules, enabling a complex series of post-translational modifications (PTMs) to mount inflammatory responses. themselves are post-translationally modified following ligand-induced activation, with this being required relay the full spectrum proinflammatory Here, we reveal indispensable roles for TLR4 Y672 Y749 phosphorylation in mounting optimal LPS-inducible responses primary mouse macrophages. LPS promotes at both tyrosine residues,...
Conventional assays to probe signaling protein interactions and function involve measurement of luciferase reporter expression within the bulk cell population, with lack control over target-protein level. To address this issue, we have developed a rapid robust flow cytometric assay for analysis function. A fluorescent tagging target enables simultaneous assessment individual cells. We applied our technique variants lipopolysaccharide receptor Toll-like 4 (TLR4) its adapter MyD88, using...
Myeloid differentiation primary response gene 88 (MyD88) and MyD88 adaptor-like/TIRAP (MAL) are Toll-like receptor (TLR) adaptors that signal to induce proinflammatory cytokine production.The TLR responds a pathogen or endogenous molecules identified as 'dangerous' our cells.This results in triggering the cytoplasmic/interleukin-1 followed by recruitment of adaptor proteins MAL MyD88.We previously observed TIR domain (MAL ) forms filaments vitro induces formation crystalline higher-order...
TIR (Toll/interleukin-1 receptor) domains are widely distributed in animals, plants and bacteria, function through self-association homotypic interactions with other [1].Across phyla, these feature proteins immune functions -TLRs (Toll-like receptors), IL-1Rs (interleukin-1 receptors) their adaptor animals; NLRs (nucleotide-binding, leucine-rich repeat plants; antiphage defence bacteria.Although long assumed to only have protein interaction functions, the across kingdoms also...
MyD88 and MAL are Toll-like receptor (TLR) adaptors that signal to induce pro-inflammatory cytokine production.We previously observed the TIR domain of (MAL ) forms filaments in vitro induces formation crystalline higher-order assemblies (MyD88 ).Due their crystal size, conventional crystallography proved be challenging.However, through serial femtosecond (SFX) we were able determine structure crystals.Here, present SFX assembly, which revealed a biological relevant two-stranded higher order...