A5078138847- Lymphoma Diagnosis and Treatment
- Immune Cell Function and Interaction
- Viral-associated cancers and disorders
- Cancer Immunotherapy and Biomarkers
- Acute Lymphoblastic Leukemia research
- Natural product bioactivities and synthesis
- IL-33, ST2, and ILC Pathways
- Viral Infections and Vectors
- Genetic factors in colorectal cancer
- RNA Research and Splicing
- Cytomegalovirus and herpesvirus research
- Reproductive System and Pregnancy
- Lung Cancer Treatments and Mutations
- Cell death mechanisms and regulation
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Mosquito-borne diseases and control
- DNA Repair Mechanisms
- Molecular Biology Techniques and Applications
- Orthopaedic implants and arthroplasty
- Natural Compounds in Disease Treatment
- Parvovirus B19 Infection Studies
- Viral Infections and Outbreaks Research
Instituto Nacional do Câncer
2011-2022
Oncovir (United States)
2022
Classical Hodgkin lymphoma (cHL) is characterized by a small number of neoplastic cells in background reactive cells. Children and adults differ constitution functionality the immune system it possible that there may be age-related differences tumor microenvironment composition cHL. One hundred children with pediatric cHL were studied. Tumor-infiltrating lymphocytes analyzed immunohistochemistry (IHC) image analysis. Epstein-Barr virus (EBV) status was determined EBER-specific situ...
Macrophages have been implicated in the pathogenesis of classical Hodgkin lymphoma (cHL) and suggested to a negative impact on outcome. Most studies addressing role macrophages cHL relied identification by generic macrophage antigens, e.g., CD68. We therefore conducted an situ analysis polarization series 100 pediatric (pcHL) cases using double staining immunohistochemistry, combining CD68 or CD163 with pSTAT1 (M1-like) CMAF (M2-like). M1- M2-polarised microenvironment was defined excess one...
To aid understanding of primary EBV infection, we have performed an in depth analysis EBV-infected cells and local immune tonsils from infectious mononucleosis (IM) patients, We show that is present approximately 50% B-cells showing heterogeneous patterns latent viral gene expression probably reflecting different stages infection. While the vast majority EBV+ are B-cells, around 9% express T-cell antigens, with a predominance CD8+ over CD4+ cells. PD-L1 was expressed by median 14% The...
Interleukin-10 (IL10) is an immune regulatory cytokine. Single nucleotide polymorphisms (SNPs) in IL10 promoter have been associated with prognosis adult classical Hodgkin lymphoma (cHL). We analyzed SNPs -1082 and -592 respect of therapy response, gene expression tumor microenvironment (TME) composition 98 pediatric patients cHL. As confirmatory results, we found that -1082AA/AG; -592CC genotypes ATA haplotype were unfavourable prognosis: Progression-free survival (PFS) was shorter...
The search for clinically relevant molecular markers in classical Hodgkin lymphoma (cHL) is hampered by the histopathological complexity of disease, resulting from admixture a small number neoplastic and Reed-Sternberg (H-RS) cells with an abundant heterogeneous microenvironment. In this study, we evaluated gene expression profiles 11 selected genes previously proposed as score adult cHL, aiming to validate its application pediatric setting. Assays were performed RT-qPCR formalin-fixed...
Classical Hodgkin lymphoma (cHL) cells overexpress heat-shock protein 90 (HSP90), an important intracellular signaling hub regulating cell survival, which is emerging as a promising therapeutic target. Here, we report the antitumor effect of celastrol, anti-inflammatory compound and recognized HSP90 inhibitor, in Reed–Sternberg lines. Two disparate responses were recorded. In KM-H2 cells, celastrol inhibited proliferation, induced G0/G1 arrest, triggered apoptosis through activation...
Chikungunya virus (CHIKV) is an arbovirus that can cause chronic and debilitating manifestations. The first autochthonous case in Rio de Janeiro state was diagnosed 2015, outbreak declared 2016.The aim of this work to evaluate CHIKV viral load serum, plasma urine cancer patients determine the best sample for diagnosis, as well perform molecular characterisation phylogenetic analysis circulating strains.Paired collected from 31 were tested by real-time quantitative polymerase chain reaction...
We have shown recently that in EBV+ paediatric classical Hodgkin lymphoma (cHL) the tumour microenvironment is characterized by a cytotoxic/Th1 profile and higher numbers of CD14+, CD68+ CD163+ cells when compared to EBV- cases. Our results suggested also represent heterogeneous cell population. The objectives this study were evaluate macrophage polarization cHL its impact on survival. was analysed double-immunohistochemistry based combination CD68 or CD163 with pSTAT1 (M1 macrophages), as...