A5032990613- Malaria Research and Control
- Mosquito-borne diseases and control
- Research on Leishmaniasis Studies
- Virus-based gene therapy research
- Parasites and Host Interactions
- Trypanosoma species research and implications
- Drug-Induced Hepatotoxicity and Protection
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Viral Infections and Outbreaks Research
- Viral Infections and Vectors
- Drug Transport and Resistance Mechanisms
- Infectious Encephalopathies and Encephalitis
- Viral gastroenteritis research and epidemiology
- Vector-borne infectious diseases
- Prenatal Screening and Diagnostics
- Hemoglobinopathies and Related Disorders
- Cytomegalovirus and herpesvirus research
- SARS-CoV-2 and COVID-19 Research
- Hepatitis B Virus Studies
- Venomous Animal Envenomation and Studies
- Animal Virus Infections Studies
- Infectious Diseases and Tuberculosis
- Pneumonia and Respiratory Infections
- Lymphoma Diagnosis and Treatment
University of Lisbon
2023-2024
Instituto Nacional do Câncer
2017-2023
Oncovir (United States)
2022
Ministério da Saúde
2022
Fundação Oswaldo Cruz
2007-2016
Ministério da Saúde
2011
Abstract Background Effective treatment remains a mainstay of malaria control, but it is unfortunately strongly compromised by drug resistance, particularly in Plasmodium falciparum , the most important human parasite. Although P. chemoresistance well recognized all over world, limited data are available on distribution and prevalence pfcrt pfmdr1 haplotypes that mediate resistance to commonly used drugs show distinct geographic differences. Methods -infected blood samples collected 2007 at...
Increasing evidence supports the repositioning of beta-lactams for tuberculosis (TB) therapy, but further research on their interaction with conventional anti-TB agents is still warranted. Moreover, complex cell envelope
In Brazil, malaria still remains a clinically important febrile syndrome for local populations and travelers, occurring mostly in the Amazon Basin. This review aims to report main efforts employed control this disease since 1940s emergence of Plasmodium falciparum vivax chemoresistance chloroquine sulphadoxine-pyrimethamine among other drugs. Additionally, vivo, vitro molecular studies as well consequences on morbidity policy treatment guidelines were commented.
Plasmodium vivax control is now being hampered by drug resistance. Orthologous falciparum genes linked to chloroquine or sulfadoxine-pyrimethamine chemoresistance have been identified in P. parasites, but few studies performed. The goal of the present work characterise pvmdr1 and pvdhfr parasite isolates from a Brazilian endemic area where no molecular investigation had previously conducted. analysis revealed existence single (85.7%) double (14.3%) mutant haplotypes, while examination showed...
Abstract Background The goal of the present study was characterization Plasmodium falciparum genes associated to malaria drug resistance ( pfcrt , pfdhfr and pfdhps) in samples from two Brazilian localities. Methods Parasites 65 P. were genotyped using nested-PCR direct DNA sequencing. Results Six resistant sulphadoxine-pyrimethamine (SP) genotypes one haplotype SP sensitivity detected. For gene, SVMNT chloroquine (CQ)-resistant genotype detected as well CVMNK CQ-sensitive same sample...
Epstein–Barr virus (EBV) is a saliva-borne ɣ-herpesvirus associated with benign and malignant lymphoproliferation. EBV-mediated tumorigenic mechanisms are not fully understood may be related to viral genetic variations. In this work, we characterize the diversity of EBV from Brazil, assessing 82 samples derived saliva asymptomatic carriers (n = 45), biopsies reactive hyperplasia 4), lymphomas 33). Phylogenetic phylogeographic analysis entire coding region LMP-1 was performed. Additionally,...
Malaria is a major parasitic disease, affecting millions of people in endemic areas. Plasmodium falciparum parasites are responsible for the most severe cases and its resistance to anti-malarial drugs notorious. This possible obstacle effectiveness intermittent preventive treatment (IPT) based on sulfadoxine-pyrimethamine (SP) cures administrated pregnant women (IPTp) during their pregnancy. As this intervention recommended Angola since 2006, it has assessed, country, molecular profiles P....
This study was performed to better understand the genetic diversity of known polymorphisms in pfatpase6 and pfmdr1 genes before introduction ACT Brazil, order get a genotypic snapshot Plasmodium falciparum parasites that may be used as baseline reference for future studies.Parasites from P. samples collected 2002, 2004 2006-2007 were genotyped using PCR DNA sequencing at codons 86, 130, 184, 1034, 1042, 1109 1246 gene, 243, 263, 402, 431, 623, 630, 639, 683, 716, 776, 769 771 gene.A...
Parvovirus B19 (B19V) is a common human pathogen, member of the family Parvoviridae. Typically, B19V has been found to infect erythroid progenitors and cause hematological disorders, such as anemia aplastic crisis. However, persistence genomic deoxyribonucleic acid (DNA) demonstrated in tonsils, liver, skin, brain, synovial, testicular tissues well bone marrow, for both symptomatic asymptomatic subjects. Although molecular cellular mechanisms remain undefined, it raises questions about...
Abstract Background Plasmodium falciparum malaria remains a leading health problem in Africa and its control is seriously challenged by drug resistance. Although resistance to the sulphadoxine-pyrimethamine (SP) widespread, this combination an important component of programmes as intermittent preventive therapy (IPT) for pregnant women children. In Angola, patterns have been poorly characterized, IPT has employed since 2006. The aim study was assess prevalence key antifolate mediating...
Chikungunya virus (CHIKV) is an arbovirus that can cause chronic and debilitating manifestations. The first autochthonous case in Rio de Janeiro state was diagnosed 2015, outbreak declared 2016.The aim of this work to evaluate CHIKV viral load serum, plasma urine cancer patients determine the best sample for diagnosis, as well perform molecular characterisation phylogenetic analysis circulating strains.Paired collected from 31 were tested by real-time quantitative polymerase chain reaction...
(1) Background: Malaria is a public health problem worldwide. Despite global efforts to control it, antimalarial drug resistance remains great challenge. In 2009, our team identified, for the first time in Brazil, chloroquine (CQ)-susceptible Plasmodium falciparum parasites isolates from Brazilian Amazon. The present study extends those observations include survey samples 2010 2018 Amazonas and Acre states purpose of tracking pfcrt molecular changes P. parasites. (2) Objective: investigate...
Chikungunya virus (CHIKV) is a reemerging arbovirus. Besides its classical acute symptoms, rare complications such as myocarditis, hepatitis, Guillain-Barré syndrome, encephalitis and meningoencephalitis are being increasingly reported worldwide In the present study, we report case of lethal caused by CHIKV associated with high viral load in an immunosuppressed patient A 45-year-old woman medical history Myasthenia gravis had onset fever (Day 1), followed skin rash polyarthralgia large...
Abstract Increasing evidence supports the repositioning of beta-lactams for tuberculosis (TB) therapy. However, additional research on interaction these drugs with conventional anti-TB agents is still warranted. Since complex cell envelope Mycobacterium ( Mtb ) may pose an obstacle to diffusion beta-lactams, improved activity upon combination that inhibit synthesis outer wall elements particularly relevant. In this context, we aimed determine potential synergies between and antimycobacterial...
Desde os tempos mais remotos, vírus influenza têm sido o agente causal de doenças respiratórias letais. O seu processo replicação ocorre nas células epiteliais do trato respiratório, causando uma síndrome respiratória aguda, na qual as glicoproteínas hemaglutinina (HA) e hemaglutinina-esterase-fusão (HEF), ancoradas superfície dos A C, respectivamente, são responsáveis pelos processos fusão durante ciclo viral. Ao contrário adsorção, sialidase esterase, atividades inatas desempenhadas por...