A5054096812- Sphingolipid Metabolism and Signaling
- Dermatology and Skin Diseases
- Eicosanoids and Hypertension Pharmacology
- Advancements in Transdermal Drug Delivery
- Fatty Acid Research and Health
- Immune cells in cancer
- Immune Response and Inflammation
- Circadian rhythm and melatonin
- Congenital Diaphragmatic Hernia Studies
- Lipid Membrane Structure and Behavior
- Inflammatory Bowel Disease
- Asthma and respiratory diseases
- Neonatal Respiratory Health Research
- Exercise and Physiological Responses
- Wound Healing and Treatments
- Immunotherapy and Immune Responses
- Metabolomics and Mass Spectrometry Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Cell Function and Interaction
- Lipid metabolism and biosynthesis
- Diabetic Foot Ulcer Assessment and Management
- Hematological disorders and diagnostics
- Inflammatory mediators and NSAID effects
- Cholesterol and Lipid Metabolism
- Skin Protection and Aging
Manchester Academic Health Science Centre
2016-2024
University of Manchester
2015-2024
University of Exeter
2011-2014
Royal Devon and Exeter Hospital
2014
University of Bradford
2012
Heavitree Hospital
2011
King Edward VII Hospital
2004
University of Waterloo
1990-1991
Coventry (United Kingdom)
1974-1983
Warwickshire Hospital
1976-1981
Eicosapentaenoic acid (EPA) and docosahexaenoic (DHA) can exert antidepressant, anti-inflammatory neuroprotective properties, but the exact molecular mechanism underlying their effects is still not fully understood. We conducted both in vitro clinical investigations to test which EPA or DHA metabolites are involved these anti-inflammatory, antidepressant effects. In vitro, we used human hippocampal progenitor cell line HPC0A07/03C, pre-treated cells with either DHA, followed by interleukin...
Barrier integrity is central to the maintenance of healthy immunological homeostasis. Impaired skin barrier function linked with enhanced allergen sensitization and development diseases such as atopic dermatitis (AD), which can precede other allergic disorders, for example, food allergies asthma. Epidemiological evidence indicates that children suffering from have lower levels dietary fibre-derived short-chain fatty acids (SCFA). Using an experimental model AD-like inflammation, we report a...
This study examined the effect of exercise intensity and duration on percent blood lymphocytes in men low [LF; maximal O2 uptake (VO2max) less than 50 ml.kg-1.min-1 sedentary], moderate (MF; VO2max = 50-60 recreationally active), high (HF; greater 60 recent training history) fitness. Thirty healthy adult (aged 20-31 yr) participated four randomly ordered cycle ergometer rides: ride 1 (65% VO2max, 30 min), 2 (30% 3 (75% 4 120 min). Blood samples were drawn at various times before after...
Ceramides are important for skin health, with a multitude of species found in both dermis and epidermis. The epidermis contains linoleic acid-Ester-linked Omega-hydroxylated ceramides 6-Hydroxy-sphingosine, Sphingosine Phytosphingosine bases (CER[EOH], CER[EOS] CER[EOP], respectively), that crucial the formation epidermal barrier, conferring protection from environmental factors preventing trans-epidermal water loss. Furthermore, large number ceramides, derivatives same sphingoid various...
Acute inflammation is characterized by granulocyte infiltration followed efferocytosing mononuclear phagocytes, which pave the way for inflammatory resolution. Until now, it was believed that resolution then leads back to homeostasis, physiological state tissues experience before occurred. However, we discovered triggered a prolonged phase of immune suppression mediated prostanoids. Specifically, once switched off, natural killer cells, secreting interferon γ (IFNγ), infiltrated...
Rheumatoid arthritis (RA) is considered a T cell driven autoimmune disease, therefore, the ability of B depleting biologics, e.g., anti-CD20 antibodies, to alleviate RA unclear. This study examined proportions IL-17-secreting lymphocytes in blood healthy subjects and patients determined if Th17 cells belong CD20+ subset cells. Fluorescence-activated sorting confocal microscopy verified CD3, CD4/CD8 CD20-staining IL-17 secretion was using commercial assay. In blood, median percentage total...
Abstract Acne skin demonstrates increased transepidermal water loss ( TEWL ) compared with healthy skin, which may be due, in part, to altered ceramide (CER) levels. We analysed ceramides the stratum corneum of and acne studied seasonal variation over course a year. Using ultraperformance liquid chromatography electrospray ionisation tandem mass spectrometry UPLC / ESI ‐ MS ), we identified 283 ceramides. Acne‐affected demonstrated overall lower levels ceramides, notable reductions CER [ NH...
This study investigated the effects of intensity and duration exercise on lymphocyte proliferation as a measure immunologic function in men defined fitness. Three fitness groups--low [maximal O2 uptake (VO2max) = 44.9 +/- 1.5 ml O2.kg-1.min-1 sedentary], moderate (VO2max 55.2 1.6 recreationally active), high 63.3 1.8 endurance trained)--and mixed control group 52.4 2.3 O2.kg-1.min-1) participated study. Subjects completed four randomly ordered cycle ergometer rides: ride 1, 30 min at 65%...
The spontaneous self-assembly of biomolecules around the surface nanoparticles (NPs) once exposed to plasma and other biofluids, has been termed 'biomolecule corona'. While protein composition biomolecule corona widely characterised, interaction NPs with lipidome not fully investigated. Here, we use targeted untargeted lipidomics analyse a wide spectrum bioactive lipids adsorbed onto liposome post-incubation human plasma. Our data indicate that contains diverse mixture simple complex lipid...
Abstract Resolving inflammation is thought to return the affected tissue back homoeostasis but recent evidence supports a non-linear model of resolution involving phase prolonged immune activity. Here we show that within days following Streptococcus pneumoniae -triggered lung inflammation, there an influx antigen specific lymphocytes with memory and tissue-resident phenotype as well macrophages bearing alveolar or interstitial phenotype. The transcriptome these shows enrichment genes...
We applied systems biology approaches to investigate circadian rhythmicity in rheumatoid arthritis (RA). recruited adults (age 16–80 years old) with a clinical diagnosis of RA (active disease [DAS28 > 3.2]). Sleep profiles were determined before inpatient measurements saliva, serum, and peripheral blood mononuclear leukocytes (PBML). Transcriptome proteome analyses carried out by RNA-SEQ LC-MS/MS. Serum samples analysed targeted lipidomics, along serum from mouse collagen induced-arthritis...
Nutritional supplementation with fish oil or ω‐3 (n‐3) polyunsaturated fatty acids (PUFAs) has potential benefits for skin inflammation. Although the differential metabolism of main n‐3PUFA eicosapentaenoic acid (EPA) and docosahexaenoic (DHA) could lead to distinct activities, there are no clinical studies comparing their relative efficacy in human skin. Following a 10‐wk oral healthy volunteers using mass spectrometry‐based lipidomics, we found that mainly affected epidermal mediator...
Abstract The menopause can lead to epidermal changes that are alleviated by hormone replacement therapy (HRT). We hypothesise these could relate altered ceramide production, and oestrogen may have a role in keratinocyte metabolism. White Caucasian women were recruited into three groups: pre-menopausal (n = 7), post-menopausal 11) taking HRT 10). Blood samples assessed for levels, transepidermal water loss was measured assess skin barrier function, stratum corneum lipids sampled from...
INTRODUCTION: Myomectomy is the gold standard for surgical removal of uterine leiomyomas. Evidence-based research exploring accuracy ultrasound (US) compared to magnetic resonance imaging (MRI) preoperative mapping fibroids limited. METHODS: A retrospective chart review was conducted assess patients who underwent laparoscopic robotic-assisted myomectomy symptomatic at a single institution, by surgeon, from 2013 2023. Imaging, intraoperative, and postoperative outcomes were abstracted medical...
Haemolytic anaemia is a well-recognized complication of chronic renal failure, but in patients with acute disease it much less frequent occurrence.The earliest reports were by Hensley (1952), who described 3 glomerulonephritis complicated haemolytic anaemia, and Dacie, Mollison, Richardson, Selwyn, Shapiro (1953), whose patient atypical congenital anaemia' was found at necropsy to have nephritis'.Attention drawn this 'Haematolytisch-uramische Syndrome' Gasser, Gautier, Steck, Siebenmann,...
Sodium lauryl sulfate (SLS) and ultraviolet radiation (UVR) are two commonly encountered cutaneous inflammatory stimuli. Differing histopathological clinical features implicate involvement of alternative pathways; bioactive lipid mediators (eicosanoids, endocannabinoids sphingolipids) likely candidates for regulation the divergent responses.To assess comprehensively in SLS- UVR-induced responses, to provide a better understanding mediator pathways irritant inflammation.Buttock skin from 10...
Summary. We have compared the mortality and morbidity of low birthweight breech‐presenting singleton infants born in Coventry Maternity Hospital during years 1973 1974 with those 1979 1980. During this period changes management included an increase caesarean section rate continuing development methods intensive care for newborn infants. The neonatal has fallen but increased survival is accounted largely by handicapped value routine absence other obstetric pathology breeches unproven may be...