Periosteum has been demonstrated to have cell populations, including chondroprogenitor and osteoprogenitor cells, that can form both cartilage bone under appropriate conditions. In the present study, periosteum was harvested, expanded in culture, used repair critical size calvarial defects a rabbit model. isolated from New Zealand White rabbits, grown labeled with thymidine analog bromodeoxyuridine for later localization, seeded into resorbable polyglycolic acid scaffold matrices. Thirty...

<h2>Abstract</h2><h3>Background Context</h3> The most commonly used materials for interbody cages are titanium metal and polymer polyetheretherketone (PEEK). Both of these have demonstrated good biocompatibility. A major disadvantage associated with solid is their radiopacity, limiting the postoperative monitoring spinal fusion via standard imaging modalities. However, PEEK radiolucent, allowing a temporal assessment mass by clinicians. On other hand, hydrophobic, which can limit bony...

The clinical study conducted was a prospective, randomized, double-blind, placebo-controlled trial.The purpose of this to evaluate the effect combined magnetic fields on healing primary noninstrumented posterolateral lumbar spine fusion.Combined fields, new type biophysical stimulus, have been shown act by stimulating endogenous production growth factors that regulate process. This is first assess an electromagnetic stimulus fusion surgery as well evaluation adjunctive fusion.This...

Abstract This article summarizes the results of a three-dimensional study changes in morphology L6 rat vertebra at 120 days after ovariectomy (OVX), with estrogen replacement therapy used as positive control. Synchrotron radiation microtomography was to quantify structural parameters defining trabecular bone architecture, while finite-element methods were explore relationships between these and compressive elastic behavior vertebrae. There 22% decrease volume (TBV) 19% decline mean thickness...

In vitro exposure to low-energy, combined magnetic fields (CMF) increased the release of insulin-like growth factor (IGF)-II from human TE-85 osteosarcoma cells. Short-term CMF only 10 min IGF-II levels in conditioned medium 1 h post exposure. were measured with a radioreceptor assay using H-35 cells that contain abundant but not IGF-I receptors. This also uses recently validated BioGel P-10 acid gel filtration method remove IGF binding protein before quantitation either or IGF-II. addition...

Estrogens play an important but poorly understood role in the maintenance of skeletal mass. Whereas mechanisms estrogen action on bone may be complex, finding that osteoblasts express receptors suggests this class hormones exerts direct effects cells. To understand how estrogens regulate osteoblastic function, physiologically active metabolite 17 beta-estradiol was tested to determine its well characterized murine cell-line MC3T3-E1. Experiments were designed identify activities associated...

Cellular morphology changes, which appear related to dedifferentiation (despecialization), have been produced in vitro the nucleated red blood cell of frog. This has achieved by controlled alterations electrochemical environment these living cells, both a selective modification ionic concentrations an isotonic amphibian Ringer solution, and electromagnetic induction pulsating current having specific waveform parameters. Laser flow microfluorometry shows that modified solution is able, per...

This multicenter clinical study was performed to assess the safety and effectiveness of Trinity Evolution® (TE), a viable cellular bone allograft, in combination with PEEK interbody spacer supplemental anterior fixation patients undergoing cervical discectomy fusion (ACDF). In prospective, multi-center study, 31 that presented symptomatic degeneration at one vertebral level underwent ACDF (Orthofix, Inc., Lewisville, TX, USA) fixation. addition all had graft substitute, Evolution...

Abstract Human osteosarcoma–derived osteoblast-like cells, TE-85, were used to assess the effect of a low frequency alternating magnetic field in combination with controlled static (combined fields, CMF) on insulin-like growth factor receptor regulation. In our culture system, application 15.3 Hz CMF induces calculated maximum electrical potential media 10−5 V/m. Initial characterization TE-85 cells demonstrated that (a) contain both type I (IGF-I) and IGF-II receptors (b) dose dependence...

Previous studies have shown that a single injection of thrombin peptide (TP508) accelerates fracture repair in closed rat femoral model. The present study was conducted to elucidate the molecular mechanisms TP508 action using Affymetrix genome-scale profiling and link early gene expression changes histology bone strength changes. Treatment fractures with accelerated as determined by destructive torsion testing. Blinded histological analysis demonstrated TP508-treated callus had significant...

Background: Cellular bone allograft (CBA) possesses osteogenic, osteoinductive, and osteoconductive elements essential for healing. The purpose of this study was to assess the safety effectiveness CBA in foot and/or ankle arthrodeses. Methods: A prospective, multicenter, open-label clinical trial using performed. At 6 weeks at 3, 6, 12 months, imaging performed subject’s pain, function, quality life (QOL) status (Visual Analog Scale, American Orthopaedic Foot &amp; Ankle Society Hindfoot...

The post-surgery integrity of the tendons and muscle quality are two major factors in success rotator cuff (RC) repair. Though surgical techniques for repair have significantly improved past decades, there no effective treatments to improve tendon-to-bone healing after at this point time. Pulsed electromagnetic fields (PEMF) previously been used promoting fracture healing. Previous studies shown that PEMF has a positive role osteoblast precursors proliferation differentiation. However, PEMFs...

Rotator cuff tears are common musculoskeletal injuries often requiring surgical intervention with high failure rates. Currently, pulsed electromagnetic fields (PEMFs) used for treatment of long-bone fracture and lumbar cervical spine fusion surgery. Clinical studies examining the effects PEMF on soft tissue healing show promising results. Therefore, we investigated role rotator using a rat repair model. We hypothesized that exposure following would improve tendon mechanical properties,...

The synthetic peptide, TP508 (Chrysalin), was delivered to rabbit segmental bone defects in biodegradable controlled-release PLGA microspheres determine its potential efficacy for enhancing healing of non-critically and critically sized defects. Non-critically radial were created the forelimbs New Zealand White rabbits, which randomized into three treatment groups receiving 10, 50 100 microg doses right radius control (without TP508) left radius. Torsional testing radii at six weeks showed a...

Abstract Background Thrombin related peptide (TP508) is a 23 amino-acid synthetic that represents portion of the receptor-binding domain thrombin molecule. Previous studies have shown TP508 can accelerate musculoskeletal tissue repair including fracture healing. Objectives The aim this study was to investigate effect on healing in murine model representing high energy situation. Methods Eighty CD 1 mice underwent controlled quadriceps muscle crush and open transverse mid diaphyseal femoral...

Trinity Evolution® (TE), a viable cellular bone allograft, previously demonstrated high fusion rates and no safety-related concerns after single-level anterior cervical discectomy (ACDF) procedures. This prospective multicenter clinical study was performed to assess the radiographic outcomes of TE in subjects undergoing two-level ACDF In prospective, study, 40 that presented with symptomatic degeneration at two adjacent vertebral levels underwent instrumented using autograft substitute...