A5053365297- Genomics and Chromatin Dynamics
- Cancer-related gene regulation
- Kruppel-like factors research
- Williams Syndrome Research
- CRISPR and Genetic Engineering
- Genetic Syndromes and Imprinting
- Retinal Development and Disorders
- Cancer-related Molecular Pathways
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Ocular Oncology and Treatments
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Cellular Mechanics and Interactions
- Infant Health and Development
- Chromosomal and Genetic Variations
- Genetics and Neurodevelopmental Disorders
- Multilevel Inverters and Converters
- Child Nutrition and Feeding Issues
- Sleep and related disorders
- Gene expression and cancer classification
- Electric Motor Design and Analysis
- Pluripotent Stem Cells Research
Michigan State University
2019-2024
University of Wisconsin–Milwaukee
2016
Abstract Retinoblastoma tumor suppressor proteins (Rb) are highly conserved metazoan transcriptional corepressors involved in regulating the expression of thousands genes. The vertebrate lineage and Drosophila genus independently experienced an Rb gene duplication event, leading to several paralogs whose unique redundant roles regulation remain be fully explored. Here, we used a novel CRISPRi system identify significance paralogy family. We engineered dCas9 fusions fly Rbf1 Rbf2 deployed...
Pleiotropically acting eukaryotic corepressors such as retinoblastoma and SIN3 have been found to physically interact with many widely expressed "housekeeping" genes. Evidence suggests that their roles at these loci are not provide binary on/off switches, is observed highly cell-type specific genes, but rather serve governors, directly modulating expression within certain bounds, while shutting down gene expression. This sort of regulation challenging study, the differential levels can be...
Evolution of sequence-specific transcription factors clearly drives lineage-specific innovations, but less is known about how changes in the central transcriptional machinery may contribute to evolutionary transformations. In particular, regulators are rich intrinsically disordered regions that appear be magnets for innovation. The C-terminal Binding Protein (CtBP) a corepressor derived from an ancestral lineage alpha hydroxyacid dehydrogenases; it found mammals and invertebrates, features...
Retinoblastoma tumor suppressor proteins regulate the key transition from G1 to S phase of cell cycle. The mammalian Rb family comprises Rb, p107, and p130, with overlapping unique roles in gene regulation. Drosophila experienced an independent duplication event, leading Rbf1 Rbf2 paralogs. To uncover significance paralogy family, we used CRISPRi. We engineered dCas9 fusions Rbf2, deployed them promoters developing tissue study their relative impacts on expression. On some genes, both...
The C-terminal Binding Protein (CtBP) is a transcriptional corepressor that plays critical roles in development, tumorigenesis, and cell fate. CtBP proteins are structurally similar to alpha hydroxyacid dehydrogenases feature prominent intrinsically disordered region the C-terminus. In mammalian system, lacking Domain (CTD) able function as regulators oligomerize, putting into question significance of this unstructured domain for gene regulation. Yet, presence an CTD ∼100 residues, including...
A rapid measure of anxiety, such as distress in novel toileting situations, may identify at-risk children community practice. Parents 8- to 12-years-old completed demographics surveys, a retrospective toilet fear (TF) survey, the Spence Anxiety Scale, and Pediatric Symptom Checklist-17. 17 percent reportedly had persistent toilets, associated with higher anxiety attention problems scores. The presence TF be meaningful indicator when evaluating child’s risk for anxiety. Because difficulties...
After a COVID-related hiatus, the fifth biennial symposium on Evolution and Core Processes in Gene Regulation met at Stowers Institute Kansas City, Missouri July 21 to 24, 2022. This symposium, sponsored by American Society for Biochemistry Molecular Biology (ASBMB), featured experts gene regulation evolutionary biology. Topic areas covered enhancer evolution, cis-regulatory code, regulatory variation, with an overall focus bringing power of deep learning (DL) decipher DNA sequence...
The C-terminal Binding Protein (CtBP) is a transcriptional corepressor that plays critical roles in development, tumorigenesis, and cell fate. CtBP proteins are structurally similar to alpha hydroxyacid dehydrogenases feature prominent intrinsically disordered region the C-terminus. In mammalian system, lacking Domain (CTD) able function as regulators oligomerize, putting into question significance of this unstructured domain for gene regulation. Yet, presence an CTD ~100 residues, including...
Abstract Evolution of sequence-specific transcription factors clearly drives lineage-specific innovations, but less is known about how changes in the central transcriptional machinery may contribute to evolutionary transformations. In particular, regulators are rich intrinsically disordered regions that appear be magnets for innovation. The C-terminal Binding Protein (CtBP) a corepressor derived from an ancestral lineage alpha hydroxyacid dehydrogenases; it found mammals and invertebrates,...
Transcriptional repressors are often thought of as mediators decisive on/off activity that underlies cell-type, or condition-specific, gene expression. However, our recent assessments global regulators Retinoblastoma (Rb) and SIN3 led to the realization another important, but overlooked repression involves measured critical modulation expression is considerably less than two-fold. This so-called “soft repression” promoter-proximal regulation by Rb may interfere with only a subset incoming...