A5082874707- Tendon Structure and Treatment
- Rheumatoid Arthritis Research and Therapies
- Osteoarthritis Treatment and Mechanisms
- Inflammatory mediators and NSAID effects
- Cytokine Signaling Pathways and Interactions
- Molecular Biology Techniques and Applications
- Psoriasis: Treatment and Pathogenesis
- Shoulder Injury and Treatment
- Exercise and Physiological Responses
- Surgical Sutures and Adhesives
- Cardiovascular Effects of Exercise
- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- Heat shock proteins research
- Musculoskeletal synovial abnormalities and treatments
- Total Knee Arthroplasty Outcomes
- Autoimmune and Inflammatory Disorders Research
- Silk-based biomaterials and applications
- Salivary Gland Disorders and Functions
- Spaceflight effects on biology
- Dermatology and Skin Diseases
- Analytical Chemistry and Chromatography
- Cellular Mechanics and Interactions
- Additive Manufacturing and 3D Printing Technologies
- Conferences and Exhibitions Management
University of Oxford
2018-2025
Nuffield Orthopaedic Centre
2019-2025
Utrecht University
2015
Increased interleukin (IL)-17A has been identified in joints affected by osteoarthritis (OA), but it is unclear how IL-17A, and its family members IL-17AF IL-17F, can contribute to human OA pathophysiology. Therefore, we aimed evaluate the gene expression signalling pathway activation effects of different IL-17 chondrocytes synovial fibroblasts derived from cartilage synovium patients with end-stage knee OA. Immunohistochemistry staining confirmed that receptor A (IL-17RA) IL-17RC are...
Abstract The molecular and cellular basis of health in human tendons remains poorly understood. Among tendons, hamstring tendon has markedly low pathology can provide a prototypic healthy reference. aim this study was to determine the transcriptomes location all cell types tendon. Using single nucleus RNA sequencing, we profiled 10 533 nuclei from four donors identified 12 distinct types. We confirmed presence two fibroblast types, endothelial cells, mural immune previously unreported...
For more than 20 years, robotic bioreactor systems have facilitated the growth of tissue-engineered constructs using mechanical stimulation. However, we are still unable to produce functional grafts that can translate into clinical use. Humanoid robots offer prospect providing physiologically-relevant stimulation and implants which may expedite their deployment. To investigate feasibility a humanoid bioreactor, designed flexible chamber be attached modified musculoskeletal (MSK) robot...
Abstract Tendon ruptures in humans have often been studied during the chronic phase of injury, particularly context rotator cuff disease. However, early response to acute tendon remains less investigated. Quadriceps tendons, which require prompt surgical treatment, offer a model investigate this response. Therefore, study aimed explore cellular changes ruptured compared healthy human quadriceps tendons. samples were collected from patients undergoing fracture repair (healthy) or surgery...
Abstract Objectives Osteoarthritis (OA) is increasingly recognised as a whole joint disease, with an important role for synovium. However, the repertoire of immune cells and fibroblasts that constitute OA synovium remains understudied. This study aims to characterise cellular composition advanced explore potential correlations between different cell types patient demographics or clinical scores. Methods Synovium, collected from 10 patients during total knee replacement surgery, was...
Interleukin (IL)-17A, a pro-inflammatory cytokine that is linked to the pathology of several inflammatory diseases, has been shown be upregulated in early human tendinopathy and mediate tissue remodelling events. However, it remains unclear which cells tendons can respond IL-17A, how its family members IL-17F IL-17AF, affect intracellular signalling activation mRNA expression healthy diseased tendon-derived fibroblasts. Using well-phenotyped tendon samples, we show IL-17A receptors IL-17RA...
Biomaterial augmentation of surgically repaired rotator cuff tendon tears aims to improve the high failure rates (∼40%) traditional repairs. Biomaterials that can alter cellular phenotypes through provision microscale topographical cues are now under development. We aimed systematically evaluate effect topographic architecture on phenotype fibroblasts from healthy and diseased tendons. Electrospun polydioxanone scaffolds with fiber diameters ranging 300 4000 nm, in either a highly aligned or...
Abstract Tendon ruptures in humans have regularly been studied during the chronic phase of injury. However, early response to injury remains less investigated. Quadriceps tendons, which require prompt surgical treatment, offer a model investigate this response. Therefore, study aimed explore cellular changes ruptured compared healthy human quadriceps tendons. tendon samples were collected from patients undergoing tibial shaft fracture repair (healthy) or surgery for complete rupture...
Recurrent tears after surgical tendon repair remain common. Repair failures can be partly attributed to the use of sutures not designed for cellular niche nor promotion processes. Synthetic electrospun materials mechanically support whilst providing topographical cues that regulate cell behaviour. Here, a novel suture made from twisted polydioxanone (PDO) polymer filaments is compared PDS II, clinically-used PDO currently utilised in repair. We evaluated ability these attachment and...
Next generation sequencing, especially single cell RNA Sequencing (scRNA-Seq) using enzymatic digestion, has revolutionised our ability to study tissues. However, several problems remain as these techniques have traditionally been used for tissues from which substantial numbers are easily liberated. Tissues with low-cellularity and a extracellular matrix present significant challenge scRNA-Seq approaches due stress responses digestion. Here, we describe robust protocol the isolation of...
The collection of (human) tissue is essential for many aspects biomedical research. In order to maintain quality and achieve reproducible representative results, it critical that the collected in a consistent manner, especially when by different people, at locations, and/or over long period time. This particularly important snap-frozen tissue, which can be stored periods time before use range experiments including bulk single nucleus RNA sequencing imaging. Therefore, this protocol provides...
Abstract Increased interleukin (IL)-17A has been identified in joints affected by osteoarthritis (OA), but it is unclear how IL-17A, and its family members IL-17AF IL-17F, can contribute to human OA pathophysiology. Therefore, we aimed evaluate the gene expression signalling pathway activation effects of different IL-17 fibroblasts derived from cartilage synovium patients with end-stage knee OA. Immunohistochemistry staining confirmed that receptors A (IL-17RA) IL-17RC are expressed...
Abstract The molecular and cellular basis of health in human tendons remains poorly understood. Amongst tendons, the hamstrings are least likely to be injured or degenerate, providing a prototypic healthy tendon reference. aim this study was define transcriptome location all cell types hamstring tendon. We profiled transcriptomes 10,533 nuclei from 4 donors using single-nucleus RNA sequencing (snRNA-seq) identified 12 distinct types. confirmed presence two fibroblast types, endothelial...
This protocol is used the Tendon Seed Network to extract nuclei from small tissue biopsies of tissue. For larger pieces opf we use GentleMACS protocol. an iteration various extraction protocols (Martelotto, Humphreys, Macosko) developed specifically for tendon single-cell RNA-seq experiments, using drop-seq based techniques. The can be isolate both (less than 3mmx3mm) has optional (but recommended) FACS isolation step if sample contains lots debris that make counting difficult and blockages...