A5012317559- Plant Stress Responses and Tolerance
- Plant responses to elevated CO2
- Genetic Mapping and Diversity in Plants and Animals
- Rice Cultivation and Yield Improvement
- Molecular Biology Techniques and Applications
- GABA and Rice Research
- Plant Water Relations and Carbon Dynamics
- Genomics and Phylogenetic Studies
- RNA Research and Splicing
- Crop Yield and Soil Fertility
- Plant Genetic and Mutation Studies
- Seed Germination and Physiology
- Plant responses to water stress
- Wheat and Barley Genetics and Pathology
- Heat shock proteins research
- Plant and Fungal Interactions Research
- Bioenergy crop production and management
Texas Tech University
2023-2024
Naval Facilities Engineering Systems Command
2023
Kansas State University
2017-2022
International Rice Research Institute
2011
ABSTRACT Flowering in wheat ( Triticum aestivum L.) is highly sensitive to heat stress. Eleven spring genotypes were exposed stress (34/16°C, day/night temperature) during flowering investigate the impact on time of day flowering, seed set, grain yield, and quality under controlled environment chambers. In general, all 11 recorded peak cooler hours (i.e. either early morning or late evening). The trend was more pronounced stress, providing first evidence an alternative mechanism escape...
Using existing protocols, RNA extracted from seeds rich in starch often results poor quality RNA, making it inappropriate for downstream applications. Though some methods are proposed extracting plant tissue and other polysaccharides, they invariably yield less RNA. In order to obtain high tissues including roots a modified SDS-LiCl method was compared with methods, TRIZOL kit (Invitrogen), Plant RNeasy mini (Qiagen), Furtado (2014) method, CTAB-LiCl method. Modifications the extraction...
The predicted increase in global temperatures will the probability of exposing sorghum [ Sorghum bicolor (L.) Moench] to heat stress during critical reproductive developmental stages, such as flowering and post‐flowering periods. Greenhouse field studies were conducted quantify impact on pollen germination other physiological processes affecting grain yield. Pollen collected from 24 diverse genotypes grown under greenhouse conditions tested for their tolerance stress. Using same set...
Abstract Background Rice is a major staple food crop for more than half the world’s population. As global population expected to reach 9.7 billion by 2050, increasing production of high-quality rice needed meet anticipated increased demand. However, environmental changes, especially temperatures, can affect grain yield and quality. Heat stress one causes an proportion chalkiness in rice, which compromises quality reduces market value. Researchers have identified 140 quantitative trait loci...
Drought affected rice areas are predicted to double by the end of this century, demanding greater tolerance in widely adapted mega-varieties. Progress on incorporating better drought has been slow due lack appropriate phenotyping protocols. Furthermore, existing protocols do not consider effect and heat interactions, especially during critical flowering stage, which could lead false conclusion about tolerance. Screening germplasm mapping-populations identify quantitative trait loci...
SUMMARY RNA‐Sequencing is widely used to investigate changes in gene expression at the transcription level plants. Most plant RNA‐Seq analysis pipelines base normalization approaches on assumption that total transcript levels do not vary between samples. However, this has been demonstrated. In fact, many common experimental treatments and genetic alterations affect efficiency or RNA stability, resulting unequal abundance. The addition of synthetic controls a simple correction for variation...
Summary Increasing populations and temperatures are expected to escalate food demands beyond production capacities, the development of maize lines with better performance under heat stress is desirable. Here, we report that constitutive ectopic expression a heterologous glutaredoxin S17 from Arabidopsis thaliana ( AtGRXS17 ) can provide thermotolerance in through enhanced chaperone activity modulation stress‐associated gene expression. The thermotolerant had increased protection against...
Sorghum is one of the four major C4 crops that are considered to be tolerant environmental extremes. shows distinct growth responses temperature stress depending on sensitivity genetic background. About half transcripts in sorghum exhibit diurnal rhythmic expressions emphasizing significant coordination with environment. However, an understanding how molecular dynamics contribute genotype-specific context time day not known. We examined whether and impact gene expression thermo-sensitive...
Abstract Increasing temperatures can severely affect wheat ( Triticum aestivum L.) production, particularly when it coincides with the grain‐filling period. Heat stress induces rapid senescence resulting in early maturity and shortened In this study, applicability of vivo chlorophyll fluorescence (Chl‐F) index to track rate flag leaves spikes exposed heat were investigated. Seven winter varieties post‐flowering using growth chambers [35/15 °C (heat stress) 25/15 (control) day/night] unique...
Abstract Sorghum [ bicolor (L.) Moench] is highly sensitive to chilling temperatures (<15°C soil temperatures) during germination and early seedling establishment. Hence, the main objectives of this research were (a) validate potential level tolerance in selected sorghum genotypes, (b) determine effective concentrations safeners for improving (c) if safener combinations further enhance compared individual treatments. Four experiments conducted with five four (oxabetrinil, fluxofenim,...
Abstract The global average daily minimum temperatures are increasing at a quicker pace than the maximum temperatures, which predicted to increase in severity impacting food production. This study focuses on elucidating physiological and transcriptional response high night‐time temperature (HNT) stress 12 US commercial maize ( Zea mays ) hybrids using unique field‐based infrastructure. Our experimental objectives were (i) impose an accurate uniformly distributed post‐flowering HNT of +4.0°C...
Abstract Sorghum is one of the four major C4 crops that are considered to be tolerant environmental extremes. shows distinct growth responses temperature stress depending on sensitivity genetic background. About half transcripts in sorghum exhibit diurnal rhythmic expressions emphasizing significant coordination with environment. However, an understanding how molecular dynamics contribute genotype-specific context time day not known. We examined whether and impact gene expression...
Abstract RNA-Sequencing is widely used to investigate changes in gene expression at the transcription level plants. Most plant RNA-Seq analysis pipelines base normalization approaches on assumption that total transcript levels do not vary between samples. However, this has been demonstrated. In fact, many common experimental treatments and genetic alterations affect efficiency or RNA stability, resulting unequal abundance. The addition of synthetic controls a simple correction for variation...