A5027760463- Biosensors and Analytical Detection
- CRISPR and Genetic Engineering
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced biosensing and bioanalysis techniques
- Viral Infections and Immunology Research
- Microfluidic and Capillary Electrophoresis Applications
- Biosimilars and Bioanalytical Methods
- Genomics and Rare Diseases
- Antiplatelet Therapy and Cardiovascular Diseases
- Protein purification and stability
- Electrowetting and Microfluidic Technologies
South China University of Technology
2025
Beike Biotechnology (China)
2025
Xiamen University
2022-2024
CRISPR-Cas12a (Cpf1) is widely used for pathogen detection. However, most Cas12a nucleic acid detection methods are limited by a PAM sequence requirement. Moreover, preamplification and cleavage separate. Here, we developed one-step RPA-CRISPR (ORCD) system unrestricted the with high sensitivity specificity that offers one-tube, rapid, visually observable of acids. In this system, RPA amplification performed simultaneously, without separate product transfer steps, 0.2 copies/μL DNA 0.4 RNA...
Abstract Objectives β-thalassemias, caused by mutations in HBB , are hereditary blood disorders that impose a significant global health burden. Detecting these through accurate genetic analysis is essential. This study aimed to create panel of cell type reference materials for β-thalassemia testing using prime editing (PE), flexible and precise genomic method. Methods PE3 systems were designed target specific mutations, including single nucleotide variants (SNVs) (−32 (C>A), CD 17...
Drug-metabolizing enzymes play an important role in the metabolism of drugs vivo. Their activity is factor affecting rate drug metabolism, which directly determines intensity and persistence action. Patients taking medication can be divided into different metabolic types through detection CYP2C19 drug-metabolizing enzyme gene polymorphisms, then used for guidance clopidogrel. Here, we describe a method based on real-time polymerase chain reaction (PCR). This uses multicolor melting curve...
Drug-metabolizing enzymes play an important role in the metabolism of drugs vivo. Their activity is factor affecting rate drug metabolism, which directly determines intensity and persistence action. Patients taking medication can be divided into different metabolic types through detection CYP2C19 drug-metabolizing enzyme gene polymorphisms, then used for guidance clopidogrel. Here, we describe a method based on real-time polymerase chain reaction. This uses multicolor melting curve analysis...