Gold, enigmatically represented by the target-like design of its ancient alchemical symbol, has been considered a mystical material great value for centuries. Nanoscale particles gold now command deal attention biomedical applications. Depending on their size, shape, degree aggregation, and local environment, nanoparticles can appear red, blue, or other colors. These visible colors reflect underlying coherent oscillations conduction-band electrons ("plasmons") upon irradiation with light...

Increases in the prevalence of antibiotic resistant bacteria require new approaches for treatment infectious bacterial pathogens. It is now clear that a nanotechnology-driven approach using nanoparticles to selectively target and destroy pathogenic can be successfully implemented. We have explored this by gold nanorods been covalently linked primary antibodies Gram-negative bacterium, Pseudomonas aeruginosa. find that, following nanorod attachment cell surface, exposure near-infrared...

Understanding plant interactions with nanoparticles is of increasing importance for assessing their toxicity and trophic transport. The primary objective this study was to assess uptake, biodistribution associated exposure tobacco plants (Nicotiana xanthi) gold (AuNPs). We employed synchrotron-based X-ray microanalysis absorption near-edge microspectroscopy high resolution electron microscopy localize AuNPs within plants. Results from these experiments reveal that entered through the roots...

For various applications of gold nanotechnology, long-term nanoparticle stability in solution is a major challenge. Lyophilization (freeze–drying) widely used process to convert labile protein and colloidal systems into powder for improved stability. However, the lyophilization itself may induce stresses resulting aggregation. Despite plethora studies evaluating proteins, liposomes, polymeric nanoparticles, little known about nanoparticles (GNPs) upon lyophilization. Herein, effects...

Six persons with the classical Angelman syndrome (AS) phenotype and de novo deletions of chromosome 15q11-q13 were studied to determine parental origin deletion. Four 6 patients had informative cytogenetic studies all demonstrated maternal inheritance These findings, together other reported cases 15 deletion in AS, suggest that maternally contributed leads AS phenotype. This contrasts Prader-Willi (PWS) which a similar paternally is observed. In cases, gamete effect such as genomic...

Abstract Gold nanoparticles have been used as an additive for aesthetic purposes centuries. However, only in the last decade scientists begun to understand fundamental concepts that explain fascinating optical properties nanoscale particles possess. may be tuned absorb and scatter light visible near‐infrared region of electromagnetic spectrum. These result from collective oscillations electrons along metal surface can altered by changing particles' shape, size, or environment. having a...

Respiratory syncytial virus (RSV) is a major cause of pneumonia and wheezing in infants the elderly, but to date there no licensed vaccine. We developed gold nanorod construct that displayed protective antigen virus, fusion protein (F). Nanorods conjugated RSV F were formulated as candidate vaccine preparation by covalent attachment viral using layer-by-layer approach. In vitro studies ELISA, electron microscopy circular dichroism revealed conformation-dependent epitopes maintained during...

In biological tissue, complex mechanisms of cellular response are closely linked to the mechanical environment that cells experience. The key understanding these may lie in measurement local fields near living and between cells. We have developed a novel optical technique which combines light elastically scattered from gold nanorods with digital image analysis track deformations occur vitro cells, real time, under darkfield microscopy. find measurable tension compression exist intercellular...

Abstract Prometaphase chromosome study of 12 persons with an established diagnosis the Angelman syndrome demonstrated that 5 had a 15q12 deletion appearing similar to commonly observed in Prader‐Willi syndrome. Phenotype‐karyotype correlation did not show any obvious clinical differences between those and without no overlap was apparent. Our survey suggests deletions are frequent but presence does appear distinguish different phenotypes. Experience cytogenetic predicts considerable...

We report a simple process to generate iron oxide coated gold nanorods. Gold nanorods, synthesized by our three-step seed mediated protocol, were with layer of polymer, poly(sodium 4-styrenesulfonate). The negatively charged polymer on the nanorod surface electrostatically attracted mixture aqueous iron(II) and iron(III) ions. Base-mediated coprecipitation salts was used form uniform coatings nanoparticles onto magnetic properties studied using superconducting quantum interference device...

A growing number of autopsy studies on larynges injected with polytef (Teflon) paste in the form PTFE Paste for Injection show tissue reaction relation to time. We are reporting two additional human and an excised granuloma. The represents a benign foreign-body variable though progressive encapsulation. degree also appears be variable, ranging from minimal bland marked florid. An undesirable side effect was observed one larynx studied post mortem clinical case; ie, cricothyroid space invaded...

We report the sonochemical synthesis of MOF (metal organic framework) nanoparticles 30-200 nm in size and electrospraying those particles on electrospun nanofibers to process a MOF-attached nanofibrous membrane. This membrane displayed significant selectivity towards CO2 capacity adsorbing with 4000-5000 ppm difference from mixed gas flow 1% 99% N2. Applying ultrasonic waves during offered rapid dispersion formation crystalline room temperature. The 100-200 higher surface area adsorption...

ONE OF US¹recently reviewed the development of intrachordal injection procedures in treatment chronic vocal disability. After having experimented with various substances,^2,3Arnold,⁴in 1962, proposed Teflon granules suspended glycerin. The present designation implant material is PTFE Paste for Injection (modified polytetrafluoro-ethylene, 50% weight w/w glycerin). This technique made it possible to revive original idea published by Brünings⁵in 1911...

• A patient with inoperable lung cancer developed left-sided laryngeal paralysis. Her dysphonia, difficulty swallowing, aspiration of secretions, and diminished cough reflex were improved intracordal polytef injection for the remainder her life. The foreign body reaction to implant showed giant cells, few lymphocytes, no polymorphonuclear leukocytes. This may be described as a bland, chronic type consistent age implant. No areas florid, acute found. Extrusion part through cricothyroid space...

Abstract Little is known about how age influences the ways in which cardiac fibroblasts interact with extracellular matrix. We investigated deformation of collagen substrates by neonatal and adult rat monolayer three-dimensional (3D) cultures, quantified expression three receptors [discoidin domain receptor (DDR)1, DDR2, β1 integrin] contractile protein alpha smooth muscle actin (α-SMA) these cells. report that contracted 3D significantly less than their neonate counterparts, whereas no...

TUBERCULOUS otitis media is a rare entity today, but pulmonary tuberculosis, though diminished in mortality, prevalent disease. While no longer among the leading causes of death United States, tuberculosis still one most frequent between ages 15 and 45 probably principle cause world.¹The possibility associated ear involvement should be considered. This report reviews particulars case diagnosed 1964 traces its course to May 1966. <h3>Report Case</h3> On Sept 29, 1964, 22-year-old,...

Abstract The external rhinoplasty approach extends the usual marginal nasal incisions to include a notched transcolumellar incision. This maneuver enhances surgical exposure for analysis and correction of all types bone, septum cartilage deformities. Nasal septal perforation, bone grafting hypophysectomy my be operated upon through this excellent avenue exposure.