The objective of this study was to investigate the impacts Bacillus subtilis (BS), ATCC 6051a strain, as a probiotic bacterium in broiler diets based 2 protein sources (soybean meal [SBM] and cowpea seeds [CWP]), on growth performance (GP), carcass traits, bone mineralization, microflora population (0 42 d age). SBM CWP starter, grower, finisher were tested presence or absence BS (5.0 × 1011 CFU spores g−1 feed) factorial arrangement treatments completely randomized design. Broilers randomly...

As the use of antibiotics has been banned or reduced in certain countries animal industries, search for new alternatives to and will continue be a research subject poultry several years. This study aimed evaluate effect basil, thyme sage essential oils (EO) broiler chickens’ diets. A total 120 Cobb 500 chickens aged 12 days were distributed into four homogeneous groups thirty with six replicates five broilers each, raised until 42 age. For diets, corn, soybean meal, corn gluten wheat used as...

This pilot study evaluated the effects of dietary pea inclusion as a sustainable and nutritional alternative protein source on growth performance, nitrogen balance, digestibility, intestinal health nine castrated male Topigs hybrid pigs (three pigs/group), with an initial average weight 20 ± 2.5 kg, for 45 experimental days. To conduct this digestibility study, were kept individually in metabolic cages. Three groups compared: T0 (control), T10 (10% inclusion), T20 (20% inclusion). Growth...

In this study, a comparative approach was used with the aim of filling knowledge gap regarding productive and economic parameters two native B. mori silkworm breeds (Lines C Z) fed different diets, namely mulberry leaves alone R. glutinis yeast at concentrations (1 × 109 1 107 CFU/mL). The trifactorial experiment carried out 600 silkworms during fifth instar, 300 for each breed 50 larvae per rearing tray. were randomly assigned into three feeding groups, replicates: in control group (C),...

The influence of feeding a new hybrid sorghum (ES Shamal, orange variety) in broiler chickens on growth, health, meat, and litter quality was evaluated from 1–42 d. A total 360-day-old male Ross 308 chicks (40.1 ± 2.3 g) were randomly assigned to 3 experimental diets: 100% corn-based diet (S0), partial replacement corn with 50% (S50), (S100). All diets calculated be isonitrogenous isocaloric similar content lysine, sulphur amino acids, calcium, available phosphorous. results indicated that...

This study aimed to assess the nutritional quality of cowpea seeds (cv. Doljana – CSD) and impact partially replacing soybean meal with CSD, along supplementation microencapsulated Lactobacillus salivarius (LS), on growth performance, selected carcass traits, biochemical plasma profile, tibia bone quality, microbial populations in ceca excreta broiler chickens aged 1 35 days. A total 432 mixed-sex Ross 308 chickens, one day, were randomly allocated four groups, 108 birds each group, further...

The solid-state fermentation (SSF) efficiency of Bacillus licheniformis ATCC 21424 (BL) on various agro-industrial by-products such as oilseed cakes [hemp (HSC), pumpkin (PSC), and flaxseed (FSC)] was evaluated by examining the nutritional composition, reducing sugars, in vitro protein digestibility (IVPD) for use animal nutrition. SSF significantly decreased crude protein, along with changes total carbohydrates (p < 0.05) all substrates fermented. An increase fat HSC (1.04%) FSC (1.73%)...

From gut content (ileum and cecum) of eight turkeys, 73 days old, was isolated, phenotypic identified preserved, nine strains from Lactobacillus genus (L.acidophilus IBNA 11, L. acidophilus biotype 3 12, fermentum 1, 13-18 salivarius 19) one strain Enterococcus faecium (IBNA 10).The parallel identification made by apiwebTM API50CHL V.5.1, BioMerieux (France) software, API20STREP, ABIS online software.Quantitative level spp.strains (10 6 -10 9 CFU/g intestinal content) in ecological niche...

Mustard and grapeseed meals are low-cost by-products generated by the oil industry. We aimed to investigate effects of fiber level on concentration volatile fatty acids (VFAs) in ileum cecum, as well microflora enteric methane (E-CH4) pigs. A total 70 Topigs hybrid pigs, 56 ± 3 days old, 20.96 0.26 kg live weight, were randomly allotted two feeding groups: (i) SM diet based sunflower meal; (ii) MG mustard × (MG-mixt). VFA profiles determined gas chromatography. E-CH4 was calculated using our...

The aim of the study was to characterize Bacillus subtilis ATCC 6051a strain, in order establish its probiotic utility piglet nutrition. strain assayed morphologically, culturally, biochemically, for hemolytic activity and enzymatically (amylase protease). identification analysis biochemical characteristics performed by catalase assay, API 50 CHB Biomerieux strips, apiweb V 4.0 soft (B. very good identification, 99.4% ID) ABIS online. on blood agar medium. growth evaluated two ways: static...

This study investigates the effects of Bacillus licheniformis (BL) ATCC 21424 strain, as a potential bacterial probiotic in broiler diets based on soybean meal (SBM) or cowpea seeds (CWP), growth performance (GP), bone mineralization, and intestinal/fecal microbiota status (0 to 42 d age). A 2 × factorial arrangement was employed completely randomized design, with four dietary treatments: SBM CWP without BL supplementation (1.0 1011 CFU spores g−1 feed). total 480 one-day-old mixed-sex Ross...

The direct-fed microbial (DFM) supplied as live bacteria represents an alternative to antibiotics for maintaining health and performance in weaning piglets. It was been recognized probiotic products. Early is a valuable model get improved efficiency economical profits the modern intensive swine industry, but negative factors diarrhea diminished feed intake, weight loss gastrointestinal disturbances which can affect piglet functions with serious economic costs. To resolve postweaning...

This study shows the effects of dietary hemp seed oil on milk composition, blood immunoglobulins (Ig), and enteric methane (E-CH4) production primiparous sows, their offspring’s response at three time points. A bifactorial experiment was conducted for 21 days (d) 18 sows (195 ± 3 old). The were fed two diets: (i) a control diet (SO) based soybean (1.6%), with an 18.82 n-6:n-3 polyunsaturated fatty acids (PUFA) ratio; (ii) experimental (HO) 9.14 PUFA ratio. contained elevated level linoleic...

A total of 15 strains lactic acid bacteria (LAB) were isolated from the broiler chicken’s gastrointestinal tract. All isolates phenotypical and genetically identified. Among these isolates, only six biochemical (API 50 CHL ABIS soft) (16S rRNA sequencing) confirmed as Lactobacillus acidophilus, Limosilactobacillus fermentum, Levilactobacillus brevis, Ligilactobacillus salivarius. Probiotic properties, including tolerance to pH (pH 2.0 3.0), bile salts (0.3% oxgall), hemolysis activity,...

The study aimed to assess the effects of dietary supplementation microencapsulated L. acidophilus and plantarum their combination on growth performance, organ development, diarrhoea incidences, blood profiles, intestinal histomorphology microflora in weaned piglets. For that, 160 piglets with an average body weight (BW) 8.52 ± 0.15 kg were divided into four groups (40 piglets/group) allotted one treatments as follows: a basal diet (C diet) or containing 1 × 108 CFU/g (LA diet), 3 (LP both...

The objective of this study was to isolate, identify, preserve and determine the quantitative level Lactobacillus strains from gut content 45-day-old chickens broilers; test viability these preserved at 4 ºC room temperature (20 ± 2 ºC). were isolated, phenotypically identified 17 broilers. Identification performed by morphological, cultural biochemical characters examination, using apiwebTM ABIS online software. in intestinal (105 - 109 CFU/g) (from 8 days 9 months) also determined....

Fourteen lactic acid bacteria (LAB) strains were isolated from the intestinal tract and feces of piglets. Among these isolates, only twelve biochemically (API 50 CHL ABIS online for bacterial identification) genetically (16S rRNA sequencing) confirmed as Limosilactobacillus fermentum, Lactobacillus acidophilus, Lactiplantibacillus plantarum. Experiments to evaluate probiotic potential isolates including pH tolerance (pH 2.0 3.0), bile salts (0.3% ox gall) resistance, hemolysis activity,...

Guar (Cyamopsis tetragonoloba) is an annual legume tolerant to drought. meal (GM) a protein- and carbohydrate-rich co-product generated after the mechanical separation of endosperm from germ hull guar seed. GM has received considerable interest in animal feed as alternative soybean (SM). In this study, we aimed assess nitrogen (N) balance indicators, performance, carcass traits, main greenhouse gas (GHG) emissions resulting enteric fermentation (E-CH4) manure (M-CH4 N2O). Two tests were...

Abstract The study evaluated the effects of a Lactobacillus strains probiotic mix on performance, carcass traits, organs size, and intestinal microflora in broilers. A total 200 one-day-old unsexed broilers were allotted into 2 groups with 5 replicates (20 broilers/replicate). During feeding trial (35 d) fed control (basal diet) or diet plus 3% L. acidophilus plantarum , 1:1 ratio). supplementation did not significantly influence traits size at slaughter age. No effect visceral weight length...

Abstract This study was intended to assess the effect of graded replacements corn gluten meal with Brewer’s yeast ( Saccharomyces cerevisiae ), and without Bacillus megaterium supplementation, on performance, carcass characteristics, gut broilers microflora composition. A total 720 mixed sex one-day-old broiler chicks (Ross 308) were randomly assigned six dietary treatment groups (6 replicates each) in a 3 × 2 factorial arrangement, including levels (BY; 0, 25, 50%, replace meal; CGM)...

Chichens’s gastrointestinal (GI) tract presents a large bacterial diversity and can be beneficial source in order to the isolation of probiotics candidates. In this study, twentythree strains from Lactobacillus genus (one strain L. acidophilus biotype 1, eight 3, one crispatus, five fermentum two brevis 2, salivarius delbrueckii subsp. delbrueckii) were isolated, cultured, conserved ileum cecum digesta sixteen chickens, 26 days old, based on their phenotypic characters. The confirmation was...

Abstract This study was conducted to test the hypothesis that a new rye hybrid produced in Romania (Suceveana variety) could not impair performance and meat quality of broiler chickens. A total 360-day-old male Ross 308 chicks (40.1±2.3 g) were randomly assigned 3 dietary treatments replicated 6 times having 20 birds per replicate. Experimental diets formulated replace 25 50% corn with (RYE RYE 50, respectively) as energy-yielding sources, whereas control diet (CON) 100% corn-based. All be...

Abstract The study aimed to isolate, identify, preserve and evaluate the quantitative level of Lactobacillus strains from gut content faeces weaned piglets, 30-107 days old; test viability these preserved at 4°C room temperature. were isolated, phenotypically identified 20 piglets. Identification was performed by morphological, cultural biochemical character examination, using apiweb TM ABIS online software. spp. intestinal (10 6 – 10 9 CFU/g) 4 ° C temperature also determined (from 38...