The development of hybrid quantum systems is central to the advancement emerging technologies, including information science and quantum-assisted sensing. recent demonstration high-quality single-crystal diamond resonators has led significant interest in a system consisting nitrogen-vacancy centre spins that interact with resonant phonon modes macroscopic mechanical resonator through crystal strain. However, spin-strain interaction not been well characterized. Here, we demonstrate dynamic,...

There has been rapidly growing interest in hybrid quantum devices involving a solid-state spin and macroscopic mechanical oscillator.Such create exciting opportunities to mediate interactions between disparate qubits explore the regime of objects.In particular, system consisting nitrogen-vacancy defect center diamond coupled high quality factor oscillator is an appealing candidate for such device, as it utilizes highly coherent versatile properties center.In this paper, we will review recent...

Abstract Native mass spectrometry (MS) is increasingly used to provide complementary data electron microscopy (EM) for protein structure characterization. Beyond the ability measurements of gas-phase biomolecular ions, MS instruments offer purify, select, and precisely control spatial location these ions. Here we present a modified Orbitrap system capable depositing native ion beam onto EM grids. We further describe use chemical landing matrix that preserves structural integrity deposited...

Solution-exchange lithography is a new modular approach to engineer surfaces via sequential photopatterning. An array of lenses reduces features on an inkjet-printed photomask and reproduces arbitrarily complex patterns onto surfaces. In situ exchange solutions allows successive photochemical reactions without moving the substrate affords access hierarchically patterned substrates. As service our authors readers, this journal provides supporting information supplied by authors. Such...

The recent maturation of hybrid quantum devices has led to significant enhancements in the functionality a wide variety systems. In particular, harnessing mechanical resonators for manipulation and control expanded use two-level systems information science sensing. this letter, we report on monolithic device which strain fields associated with resonant vibrations diamond cantilever dynamically optical transitions single nitrogen-vacancy (NV) defect center diamond. We quantitatively...

Oil-particle interactions and oil submergence are of much interest to spill responders scientists, especially as transportation light heavy crude oils increases in North America’s coastal marine freshwater environments. This report contains an up-to-date review the state science for oil-particle aggregates (OPAs), terms their formation stability which may alter transport, fate, toxicity residual and, hence, its level ecological risk. Operational considerations—detection, containment,...

The results of a comparison one resin and activated carbon in the isolation organic compounds water are reported.

Hybrid quantum devices, in which disparate elements are combined order to achieve enhanced functionality, have received much attention recent years due their exciting potential address key problems information processing, communication, and control. Specifically, significant progress has been made the field of hybrid mechanical a qubit is coupled oscillator. High cooperativity such devices demonstrated with superconducting qubits, coupling defect qubits via crystal strain enabled novel...

Addressing mixtures and heterogeneity in structural biology requires approaches that can differentiate separate structures based on mass conformation. Mass spectrometry (MS) provides tools for measuring isolating gas-phase ions. The development of native MS including electrospray ionization allowed manipulation analysis intact noncovalent biomolecules as ions the gas phase, leading to detailed measurements heterogeneity. Conversely, transmission electron microscopy (TEM) generates images...

Abstract Coronaviruses are a diverse subfamily of viruses containing pathogens humans and animals. This replicates their RNA genomes using core polymerase complex composed viral non-structural proteins: nsp7, nsp8 nsp12. Most our understanding coronavirus molecular biology comes from betacoronaviruses like SARS-CoV SARS-CoV-2, the latter which is causative agent COVID-19. In contrast, members alphacoronavirus genus relatively understudied despite importance in human animal health. Here we...

Single-cell proteomics is a powerful approach to precisely profile protein landscapes within individual cells toward comprehensive understanding of proteomic functions and tissue cellular states. The inherent challenges associated with limited starting material demand heightened analytical sensitivity. Just as advances in sample preparation maximize the amount that makes it from cell mass spectrometer, we strive number ions make ion source detector. In isobaric tagging experiments, reporter...

We describe an apparatus for the cryogenic landing of particles from ion beam a mass spectrometer onto transmission electron microscope grids cryo-electron microscopy. This system also allows controlled formation thin films amorphous ice on grid surface. demonstrate that as compared to room temperature landings, use this device greatly improves structural preservation deposited protein-protein complexes. Furthermore, under conditions can increase diversity particle orientations, allowing...

ABSTRACT Single-cell proteomics is a powerful approach to precisely profile protein landscapes within individual cells toward comprehensive understanding of proteomic functions and tissue cellular states. The inherent challenges associated with limited starting material in single-cell analyses demands heightened analytical sensitivity. Just as advances sample preparation maximize the amount that makes it from cell mass spectrometer, we strive number ions make ion source detector. In isobaric...

Collision cross sections (CCSs) were determined from the frequency-domain linewidths in a Fourier transform electrostatic linear ion trap. With use of an ultrahigh-vacuum precision leak valve and nitrogen gas, transients recorded as background pressure mass analyzer chamber was varied between 4× 10-8 7 × 10-7 Torr. The energetic hard-sphere ion-neutral collision model, described by Xu coworkers, used to relate image charge CCS molecule. In lieu our monoisotopically isolating interest, known...

A novel gas-phase charge and mass manipulation approach is demonstrated to facilitate the measurement of high complexes within context native spectrometry. Electrospray ionization applied solutions generated under or near-native conditions has been be capable preserving biologically relevant into gas phase as multiply charged ions suitable for spectrometric analysis. However, state distributions tend narrow extensive salt adduction, heterogeneity, so on lead significantly broadened peaks....

Native mass spectrometry (MS) is usually conducted in the positive-ion mode; however, some cases, it advantageous to use negative-ion polarity. Challenges associated with native MS using ensemble measurements (i.e., measurement of many ions at a time as opposed charge and mass-to-charge ratio individual ions) include narrow state distributions potential for an overlap neighboring states. These issues can either compromise or preclude confident (and hence mass) determination. Charge...

Recently, we described the use of a chemical matrix for landing and preserving cations protein–protein complexes within mass spectrometer (MS) instrument. By glycerol-landing matrix, used negative stain transmission electron microscopy (TEM) to obtain three-dimensional (3D) reconstruction landed GroEL complexes. Here, investigate utilities other matrices their abilities land, preserve, allow direct imaging these cationic particles using TEM. We report here that poly(propylene) glycol (PPG)...

An electrostatic linear ion trap (ELIT) has been configured to allow for the simultaneous acquisition of mass spectra via Fourier transform (FT) techniques (frequency measurement) and time-of-flight (TOF; time measurement). In former case, time-domain image charge derived from a pick-up electrode in field-free region ELIT is converted frequency-domain data transformation (i.e., FT-ELIT MS). latter difference between injection into detection after release using microchannel plate (MCP)...

Mass resolution (M/ΔMFWHM) was increased by reducing the axial length of a Fourier transform electrostatic linear ion trap (FT-ELIT) mass spectrometer. The increase in corresponds directly to frequencies FT-ELIT. Increased demonstrated for equivalent transient lengths 5.25″ versus 2.625″ ELIT using isotopes [bradykinin+2H]2+ and [insulin+5H]5+ as test ions. Both bradykinin insulin show increases ~ 90% allowing baseline after only 300 ms data acquisition. Relative changes mass/charge range...

Epimers can show different biological activities and pharmacological behaviors; therefore, their separation analysis are crucial in the drug development process. Due to similar chemical physical properties, of epimers is challenging. This study demonstrates application cyclic ion mobility–mass spectrometry separate, identify, quantify dexamethasone betamethasone a binary mixture. Cyclic IMS isolated protonated dimer resulted three peaks: homodimer, heterodimer. Besides providing improved...

Isobaric tagging facilitates multiplexed experiments that can determine sequences and relative amounts of peptides in biological samples using tandem mass spectrometry (MSn). Limited reporter ion generation limits quantitative accuracy precision. As ions are susceptible to unintended fragmentation scattering by high-energy collisions, we activated with IR photons prevented successive dissociation generated parking, which altogether boosted yield up 55%. Even so, co-isolation contaminating...

We describe an apparatus for the cryogenic landing of particles from ion beam a mass spectrometer onto transmission electron microscope grids cryo-electron microscopy. This system also allows controlled formation thin films amorphous ice on grid surface. demonstrate that as compared to room temperature landings, use this device greatly improves structural preservation deposited protein-protein complexes. Further, under conditions can increase diversity particle orientations, allowing...

Quadrupole ion traps (QITs) are versatile platforms for performing experiments with gas-phase ions due to their abilities store of both polarities and conduct MSn experiments. The QIT is particularly useful as a reaction cell ion/ion reactions. In the case an experiment in QIT, multiply charged reactant may initially be relatively low m/z (e.g., < 1000) whereas product can one or more orders magnitude higher > 100,000). Several factors limit range over which conducted. These include (1)...