A5021090979- Hormonal and reproductive studies
- Estrogen and related hormone effects
- Mass Spectrometry Techniques and Applications
- Stress Responses and Cortisol
- Pesticide Residue Analysis and Safety
- Metabolomics and Mass Spectrometry Studies
- Hormonal Regulation and Hypertension
- Sexual Differentiation and Disorders
- Ovarian function and disorders
- Analytical Chemistry and Chromatography
- Folate and B Vitamins Research
- Metabolism and Genetic Disorders
- Adrenal Hormones and Disorders
- Reproductive Biology and Fertility
- Bone health and treatments
- Advanced Proteomics Techniques and Applications
- Thyroid Disorders and Treatments
- Pharmacological Effects and Assays
- Clinical Laboratory Practices and Quality Control
- Menopause: Health Impacts and Treatments
- Effects and risks of endocrine disrupting chemicals
- Hemoglobinopathies and Related Disorders
- Pharmacogenetics and Drug Metabolism
- Forensic Toxicology and Drug Analysis
- Schizophrenia research and treatment
ARUP Institute for Clinical and Experimental Pathology
2015-2024
ARUP Laboratories (United States)
2015-2024
University of Utah
2013-2024
Uppsala University
2008-2015
Science for Life Laboratory
2012-2015
University Pathologists
1995-2012
Czech Academy of Sciences, Institute of Analytical Chemistry
2012
C3J Therapeutics (United States)
2012
Brewster Place
2012
Charles R. Drew University of Medicine and Science
2006
Measurement of serum androgens is important in adult, geriatric, pediatric endocrinology, and oncology patients. We developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for simultaneous measurement androstenedione, dehydroepiandrosterone (DHEA), testosterone these patients.We spiked 200 muL or plasma with isotope-labeled internal standards performed extraction methyl t-butyl ether. then derivatized the extracts hydroxylamine analyzed them by LC-MS/MS using...
For many years, basic and clinical researchers have taken advantage of the analytical sensitivity specificity afforded by mass spectrometry in measurement proteins. Clinical laboratories are now beginning to deploy these work flows as well. assays that use proteolysis generate peptides for protein quantification characterization, synthetic stable isotope-labeled internal standard central importance. No general recommendations currently available surrounding spectrometric assays.The Proteomic...
High-sensitivity measurement of serum estrogens is important in adult and pediatric endocrinology oncology. We developed a high-sensitivity liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for simultaneous estrone (E1) estradiol (E2). Aliquots 200 muL were spiked with internal standard, extracted, derivatized dansyl chloride, analyzed by LC-MS/MS using 2-dimensional chromatographic separation. Total imprecision the method was less than 11%; limit quantitation 1 pg/mL....
BACKGROUND Measurement of serum thyroglobulin (Tg) is used to monitor patients after treatment for differentiated thyroid carcinoma (TC). Difficulty in using Tg as a biomarker the recurrence TC many stems from presence endogenous anti-Tg autoantibodies (Tg-AAbs), which can interfere with immunoassays (IAs) and cause false-negative results. METHODS We enriched samples rabbit polyclonal antiserum protein precipitation. Unrelated proteins were partially depleted process. Enriched then...
Commercial immunoassays for testosterone (Te) may give inaccurate results samples from women and children, leading to misdiagnosis inappropriate treatment. We developed a sensitive specific tandem mass spectrometric assay measurement of Te at the concentrations encountered in children.Te was extracted with methyl tert-butyl ether 100 microL serum or plasma, derivatized form an oxime, reextracted by solid-phase extraction. Instrumental analysis performed on API 4000 HPLC spectrometer...
Abstract Background: The recent interest of clinical laboratories in developing serum testosterone assays based on isotope dilution–liquid chromatography–tandem mass spectrometry (ID-LC-MS/MS) stems from the lack accuracy direct immunoassays. In this study, we assessed and state standardization (traceability) 4 published ID-LC-MS/MS procedures a method comparison with an ID–gas chromatography (GC)–MS reference measurement procedure listed database Joint Committee for Traceability Laboratory...
Methylmalonic acid (MMA) is a dicarboxylic whose concentration can be increased in blood and urine patients with an inborn error of metabolism or vitamin B(12) deficiency. We developed method for the selective analysis acids that exploits high specificity tandem mass spectrometry (MS/MS) substantial difference fragmentation patterns isomers methylmalonic succinic (SA).Dicarboxylic were extracted from samples methyl-tert-butyl ether derivatized butanolic HCl to form dibutyl esters. The...
The standard screening test for vitamin B12 deficiency, measurement of total plasma B12, has limitations sensitivity and specificity. Plasma bound to transcobalamin (holoTC) is the fraction available tissue uptake therefore been proposed as a potentially useful alternative indicator status.We compared diagnostic accuracy holoTC, combination both measures screen metabolic deficiency in an elderly cohort (age > or = 60 years). methylmalonic acid homocysteine were used indicators deficiency.Low...
Congenital adrenal hyperplasia is a group of autosomal recessive disorders caused by deficiency 1 4 enzymes required for the synthesis glucocorticoids, mineralocorticoids, and sex hormones. Analysis 11-deoxycortisol (11DC), 17-hydroxyprogesterone (17OHP), 17-hydroxypregnenolone (17OHPr), pregnenolone (Pr) in blood allows detection these enzyme defects.The steroids were extracted from 200 microL serum or plasma solid-phase extraction, derivatized to form oximes, again with methyl t-butyl...
Measurements of free thyroxine (FT4) and triiodothyronine (FT3) are important for the diagnosis monitoring thyroid diseases. Considerable differences among methods limit their clinical utility, however, accurate needed various specimens. We describe a direct equilibrium dialysis (ED)-liquid chromatography (LC)/tandem mass spectrometry (MS/MS) method FT4 FT3.ED was selected as separation step. Serum samples were dialyzed 1:1 against simple protein-free buffer 20 h at 37 degrees C. Thyroid...
A liquid chromatography-tandem mass spectrometry (LC-MS-MS) method for simultaneous analysis of six major opiates in urine, serum, plasma, whole blood, and meconium is described. The included are codeine, morphine, hydrocodone, hydromorphone, oxycodone, 6-acetylmorphine (6-AM). was compared to an in-house gas chromatography (GC)-MS LC-MS-MS performed by another laboratory. sample preparation time decreased eliminating the glucuronide hydrolysis derivatization required GC-MS analysis, as well...
Background.Age trends in estradiol and estrone levels men how lifestyle factors, comorbid conditions, testosterone, sex hormone-binding globulin affect these age remain poorly understood, were examined of the Framingham Heart Study. Methods.Estrone concentrations measured morning fasting samples using liquid chromatography tandem mass spectrometry Offspring Generation.Free was calculated a law action equation.Results.There 1,461 eligible (mean [±SD] 61.1 ± 9.5 years body index [BMI] 28.8 4.5...
Recently, LC-MS/MS was stated to be the method of choice measure sex steroids. Because information on mutual agreement methods is scarce, we compared 7 published for simultaneous measurement testosterone, androstenedione, and dehydroepiandrosterone (DHEA).We used analyze in duplicate 55 random samples from both men women. We performed Passing-Bablok regression analysis calculated Pearson correlation coefficients assess investigated with median concentration measured by all methods,...
Objective Little is known about the aetiology of polycystic ovary syndrome (PCOS). Some suggest that elevated maternal androgens during gestation play a causative role. This implies placental passage pregnancy. The aim this study to compare androgen and estrogen concentrations in serum pregnancy umbilical cord blood, between mothers with PCOS their offspring compared controls. Design Prospective case–control study. Methods Maternal blood samples were collected around 20 weeks at delivery....
Developmental exposure to low doses of the endocrine disruptor bisphenol A (BPA) is known alter bone tissue in young rodents, although how affected aged animals not well known. We have recently shown that low-dose developmental BPA increases procollagen type I N-terminal propeptide (P1NP) levels, a peptide formed during 1 collagen synthesis, plasma 5-week-old female rat offspring while male showed reduced size.To analyze phenotype at 52 weeks age and clarify whether BPA-induced increase P1NP...
Determination of urinary catecholamines (CATs) is considered important for clinical diagnosis pheochromocytoma, paraganglioma, and neuroblastoma. The major disadvantages existing tests include relatively long instrumental analysis time potential interference from drugs drug metabolites that are structurally similar to CATs.CATs were extracted a 300-microL aliquot urine by two-step liquid-liquid extraction method specific compounds containing catechol group. Chromatographic separation did not...
There is a need for increased knowledge about endogenous sex hormone levels and clinical outcomes of risk/benefit. Immunoassays have poor specificity to reliably measure low steroid concentrations in elderly.The objective the study was evaluate plasma with regard prevalent cardiovascular disease (CVD) elderly, using mass spectrometry.The conducted at university hospital research unit.Plasma samples were analyzed from 202 70-yr-olds as part large population-based study, Prospective...