A5004645256- Hormonal and reproductive studies
- Ovarian function and disorders
- Hormonal Regulation and Hypertension
- Adrenal Hormones and Disorders
- Stress Responses and Cortisol
- Sexual Differentiation and Disorders
- Adrenal and Paraganglionic Tumors
- Pituitary Gland Disorders and Treatments
- Adipose Tissue and Metabolism
- HIV-related health complications and treatments
- Metabolomics and Mass Spectrometry Studies
- Pharmacological Effects and Assays
- Diet, Metabolism, and Disease
- Cannabis and Cannabinoid Research
- Diet and metabolism studies
- Hypothalamic control of reproductive hormones
- Reproductive Biology and Fertility
- Genetic and phenotypic traits in livestock
- Growth Hormone and Insulin-like Growth Factors
- Pesticide Residue Analysis and Safety
- Gut microbiota and health
- Pharmacology and Obesity Treatment
- Estrogen and related hormone effects
- Sexual function and dysfunction studies
- Pregnancy-related medical research
University of Bologna
2016-2025
Policlinico S.Orsola-Malpighi
2014-2025
Azienda USL di Bologna
2021-2025
University of Modena and Reggio Emilia
2023
Istituti di Ricovero e Cura a Carattere Scientifico
2022
University of Belgrade
2020
The recent characterization of the gut microbiome traditional rural and foraging societies allowed us to appreciate essential co-adaptive role in complementing our physiology, opening up significant questions on how microbiota changes that have occurred industrialized urban populations may altered microbiota-host co-metabolic network, contributing growing list Western diseases. Here, we applied a targeted metabolomics approach profile fecal metabolome Hadza Tanzania, one world's few...
This study was designed to assess the steroid profiling by liquid chromatography coupled with tandem mass spectrometry in PCOS women different phenotypes.Cross-sectional study.University hospital of Bologna, Italy.A total 156 and 141 controls comparable for age were investigated. All underwent a spectrometry. Metabolic parameters also investigated hirsutism measured modified Ferriman-Gallwey (mF-G) score.Three distinct phenotypes initially defined according combination (mF-G ≥ 8) and/or high...
Human luteinizing hormone (LH) and chorionic gonadotropin (hCG) are glycoprotein hormones regulating development reproductive functions by acting on the same receptor (LHCGR). We compared LH hCG activity in gonadal cells from male mouse vitro, i.e. primary Leydig cells, which is a common tool used for bioassay. Murine naturally expressing murine (mLhr), binds human LH/hCG.Cultured were treated increasing doses of recombinant hCG, cell signaling, gene expression steroid synthesis evaluated.We...
Abstract Objective Mild autonomous cortisol secretion (MACS) has been associated with a higher prevalence of osteoporosis, although most data rely on single-center studies limited sample size. We aimed to assess the fragility fractures and contributing factors in large cohort patients adrenal incidentalomas. Design Methods Medical records 1023 incidentalomas from 1990 2019 were reviewed, 735 selected. Clinically obtained electronic radiological images closest first endocrine evaluation, such...
The elucidation of the role endocannabinoids in physiological and pathological conditions transferability importance these mediators from basic evidence into clinical practice is still hampered by indefiniteness their circulating reference intervals. In this work, we developed validated a two-dimensional LC/MS/MS method for simultaneous measurement plasma related compounds such as arachidonoyl-ethanolamide, palmitoyl-ethanolamide, oleoyl-ethanolamide, belonging to N-acyl-ethanolamide (NAE)...
Long-term follow-up studies revealed that patients with subclinical hypercortisolism (SH) due to adrenocortical adenomas have an increased incidence of cardiovascular diseases and mortality. No yet investigated the steroid profile its implications in SH. The objective study was analyze by liquid chromatography-tandem mass spectrometry sera from unilateral adenomas. This a cross-sectional study. conducted at outpatient clinic. Patients (nonsecreting, n = 66; SH, 28) 188 age- sex-matched...
Recently, LC-MS/MS was stated to be the method of choice measure sex steroids. Because information on mutual agreement methods is scarce, we compared 7 published for simultaneous measurement testosterone, androstenedione, and dehydroepiandrosterone (DHEA).We used analyze in duplicate 55 random samples from both men women. We performed Passing-Bablok regression analysis calculated Pearson correlation coefficients assess investigated with median concentration measured by all methods,...
Probiosis is considered a potential strategy to reduce antibiotics use and prevent post-weaning diarrhea (PWD). This study investigated the effect of Bacillus amyloliquefaciens DSM25840 or subtilis DSM25841 supplementation on growth, health, immunity, intestinal functionality microbial profile pigs after enterotoxigenic E. coli (ETEC) F4 challenge.Sixty-four piglets (7748 g ± 643 g) were randomly allocated four groups: control basal diet (CO); CO + 1.28 × 106 CFU/g B. (BAA); feed (BAS); 1...
Aging | doi:10.18632/aging.100912. Laura Bucci, Rita Ostan, Elisa Cevenini, Pini, Maria Scurti, Giovanni Vitale, Daniela Mari, Calogero Caruso, Paolo Sansoni, Flaminia Fanelli, Renato Pasquali, Paola Gueresi, Claudio Franceschi, Monti
The aim of this study isto assess the efficacy a very low-calorie ketogenic diet (VLCKD) method vs Mediterranean (LCD) in obese polycystic ovary syndrome (PCOS) women reproductive age.Randomized controlled open-label trial was performed study. treatment period 16 weeks; VLCKD for 8 weeks then LCD weeks, according to Pronokal® (experimental group; n = 15) (control 15). Ovulation monitoring carried out at baseline and after while clinical exam, bioelectrical impedance analysis (BIA),...
To estimate the risk of cardiovascular (CV) events (primary aim) and to evaluate long-term variation in CV factors a Caucasian population women with PCOS. Matched cohort prospective study based on 10 years follow-up. 120 PCOS diagnosed by NIH criteria reproductive age were assessed at baseline (2009) end follow-up (2020) for major minor factors. Five controls exactly matched presence/absence type 2 diabetes each participant (total number= 600) followed up relative events. Change epicardial...
Background Leucine supplementation might have therapeutic potential in preventing diet-induced obesity and improving insulin sensitivity. However, the underlying mechanisms are at present unclear. Additionally, it is unclear whether leucine be equally efficacious once has developed. Methodology/Principal Findings Male C57BL/6J mice were fed chow or a high-fat diet (HFD), supplemented not with for 17 weeks. Another group of HFD-fed (HFD-pairfat group) was food restricted order to reach an...
Physiological transient imbalance typical of adolescence needs to be distinguished from hyperandrogenism-related dysfunction. The accurate determination circulating androgens is the best indicator hyperandrogenism. However, reliable reference intervals for adolescent and young women are not available.The aim study was define androgen in analyze impact menstrual phase ovulation efficiency over profile as assessed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique.Female...
Following binding to cannabinoid receptors, endocannabinoids regulate a variety of central nervous system processes including appetite and mood. Recent evidence suggests that the systemic release these lipid metabolites can be altered by acute exercise their levels also vary across 24-h sleep-wake cycle. The present study utilized within-subject design (involving 16 normal-weight men) determine whether daytime circulating endocannabinoid concentrations differ following three nights partial...
Abstract STUDY QUESTION What are the consequences of ageing on human Leydig cell number and hormonal function? SUMMARY ANSWER significantly decreases in parallel with INSL3 expression Sertoli aged men, yet vitro androgenic potential does not appear to be compromised by advancing age. WHAT IS KNOWN ALREADY There is extensive evidence that accompanied decline serum testosterone levels, a general involution testis morphology reduced spermatogenic function. A few studies have previously...
Abstract Objectives Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is recommended for measuring circulating steroids. However, assays display technical heterogeneity. So far, reproducibility of corticosteroid LC-MS/MS measurements has received scant attention. The aim the study was to compare cortisol, 17OH-progesterone and aldosterone from nine European centers assess performance according external quality assessment (EQA) materials calibration. Methods Seventy-eight patient...
Most of the estimates prevalence hyperandrogenic states refer to general adult population. The objective study was estimate in late adolescence and youth evaluate potential independent predictors. This a cross-sectional study. conducted high schools. Patients included female students, aged 16–19 years. protocol designed with 3 possible levels participation: first level consisted self-compiled questionnaire; second added medical examination; third blood sample for laboratory testing. Liquid...
N-acylethanolamines play different roles in energy balance; anandamide (AEA) stimulates intake and storage, N-palmitoylethanolamide (PEA) counters inflammation, N-oleoylethanolamide (OEA) mediates anorectic signals lipid oxidation. Inconsistencies the association of plasma with human obesity cardiometabolic risk have emerged among previous studies, possibly caused by heterogeneous cohorts designs, unstandardized N-acylethanolamine measurements. We aimed to characterize changes profile,...
Abstract Context Steroid profiling by mass spectrometry has shown implications for diagnosis and subtyping of adrenal tumors. Objectives To investigate steroid profiles their cardiovascular correlates in a large cohort patients with nonsecreting (NS) incidentalomas autonomous cortisol secretion (ACS). Design Cohort study. Setting University hospital. Patients (n = 302) incidentally discovered masses, divided into unilateral adenoma hyperplasia ACS 46 n 52, respectively) NS 120 84,...
Abstract Background Obesity and related co-morbidities represent a major health challenge nowadays, with rapidly increasing incidence worldwide. The gut microbiome has recently emerged as key modifier of human that can affect the development progression obesity, largely due to its involvement in regulation food intake metabolism. However, there are still few studies have in-depth explored functionality obesity even fewer examined relationship eating behaviors. Methods In an attempt advance...