A5045664133- Cholesterol and Lipid Metabolism
- Sphingolipid Metabolism and Signaling
- Ovarian function and disorders
- Cancer, Lipids, and Metabolism
- Lipoproteins and Cardiovascular Health
- Atherosclerosis and Cardiovascular Diseases
- Reproductive Biology and Fertility
- Hypothalamic control of reproductive hormones
- Estrogen and related hormone effects
- Peroxisome Proliferator-Activated Receptors
- Drug Transport and Resistance Mechanisms
- Wnt/β-catenin signaling in development and cancer
- Hormonal and reproductive studies
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Caveolin-1 and cellular processes
- Adipokines, Inflammation, and Metabolic Diseases
- Muscle Physiology and Disorders
- Steroid Chemistry and Biochemistry
- Cytokine Signaling Pathways and Interactions
- Immune cells in cancer
- Lipid Membrane Structure and Behavior
- Lipid metabolism and disorders
- Clusterin in disease pathology
- Diet and metabolism studies
- Receptor Mechanisms and Signaling
University of Parma
2012-2024
University of Modena and Reggio Emilia
2012-2023
Azienda Unita' Sanitaria Locale Di Modena
2017
University Hospital Münster
2013
University of Münster
2013
Sorbonne Université
2011
Inserm
2011
University of Milan
2010
Human luteinizing hormone (LH) and chorionic gonadotropin (hCG) are glycoprotein hormones regulating development reproductive functions by acting on the same receptor (LHCGR). We compared LH hCG activity in gonadal cells from male mouse vitro, i.e. primary Leydig cells, which is a common tool used for bioassay. Murine naturally expressing murine (mLhr), binds human LH/hCG.Cultured were treated increasing doses of recombinant hCG, cell signaling, gene expression steroid synthesis evaluated.We...
To assess the role of apolipoprotein (apo) E in macrophage reverse cholesterol transport (RCT) vivo.ApoE exerts an antiatherosclerotic activity by regulating lipoprotein metabolism and promoting cell efflux. We discriminated between systemic apoE contribution using assay RCT mice. The complete absence lead to overall impairment process and, similarly, exclusively macrophages resulted reduction mobilization from plasma, liver, feces. Conversely, expression is sufficient promote normal even...
Classically, follicle-stimulating hormone receptor (FSHR)-driven cAMP-mediated signaling boosts human ovarian follicle growth and oocyte maturation. However, contradicting in vitro data suggest a different view on physiological significance of FSHR-mediated cAMP signaling. We found that the G-protein-coupled estrogen (GPER) heteromerizes with FSHR, reprogramming cAMP/death signals into proliferative stimuli fundamental for sustaining survival. In granulosa cells, survival are missing at high...
Bone and muscle have been recognized as endocrine organs since they produce secrete “hormone-like factors” that can mutually influence each other tissues, giving rise to a “bone–muscle crosstalk”. In our study, we made use of myogenic (C2C12 cells) osteogenic (2T3 cell lines investigate the effects cell-produced factors on maturation process osteoblasts. We found medium has inhibitory bone differentiation identified sclerostin one myokines produced by cells. Sclerostin is secreted...
Increasing evidence highlights the importance of novel players in Alzheimer's disease (AD) pathophysiology, including alterations lipid metabolism and neuroinflammation. Indeed, a potential involvement Proprotein convertase subtilisin/kexin type 9 (PCSK9) AD has been recently postulated. Here, we first investigated effects PCSK9 on neuroinflammation vitro. Then, examined impact genetic ablation cognitive performance severe mouse model AD. Finally, same animals evaluated effect loss Aβ...
The liver X receptors (LXRs) have been shown to affect lipoprotein plasma profile, lipid metabolism, and reverse cholesterol transport (RCT). In the present study, we investigated whether a short-term administration of synthetic LXR agonist T0901317 (T0) mice may RCT by modulating capacity promote cellular efflux. Consistent with previous data, pharmacological treatment caused significant increase macrophage-derived [3H]cholesterol content in plasma, liver, feces resulted improved release...
Sphingosine 1-phosphate (S1P) partly accounts for antiatherogenic properties of high-density lipoproteins. We previously demonstrated that FTY720, a synthetic S1P analog targeting all receptors but receptor type 2, inhibits murine atherosclerosis. Here, we addressed the identity mediating atheroprotective effects S1P.Low-density lipoprotein receptor-deficient mice on cholesterol-rich diet were given selective 1 agonist KRP-203 (3.0 mg/kg per day; 6 and 16 weeks). substantially reduced...
Scavenger receptor SR-BI significantly contributes to HDL cholesterol metabolism and atherogenesis in mice. However, the role of may not be as pronounced humans due cholesteryl ester transfer protein (CETP) activity. To address impact CETP expression on adverse effects associated with deficiency, we cross-bred our conditional knock-out mouse model transgenic almost completely restored abnormal HDL-C distribution SR-BI-deficient it did normalize elevated plasma free total ratio characteristic...
Background Sphingosine-1-phosphate (S1P) is a bioactive lysosphingolipid and constituent of high-density lipoprotein (HDL) exerting several atheroprotective effects in vitro. However, the few studies addressing anti-atherogenic S1P vivo have led to disparate results. We here examined atherosclerosis development low-density receptor (LDL-R)-deficient (LDL-R−/−) mice with elevated endogenous levels. Methods Results Sub-lethally irradiated LDL-R−/− were transplanted bone marrow deficient...
Recombinant follicle-stimulating hormone (FSH) (follitropin alfa) and biosimilar preparations are available for clinical use. They have specific FSH activity a unique glycosylation profile dependent on source cells. The aim of the study is to compare originator (reference) follitropin alfa (Gonal-f®)- with (Bemfola® Ovaleap®)-induced cellular responses in vitro. Gonadotropin N-glycosylation profiles were analyzed by ELISA lectin assay, revealing preparation specific-patterns glycan species...
Abstract Aims SR-B1 is a cholesterol transporter that exerts anti-atherogenic properties in liver and peripheral tissues mice. Bone marrow (BM) transfer studies suggested an atheroprotective role cells of haematopoietic origin. Here, we addressed the specific contribution monocyte/macrophage. Methods results We generated mice deficient for monocytes/macrophages (Lysm-Cre × SR-B1f/f) transplanted their BM into Ldlr−/− Fed cholesterol-rich diet, these displayed accelerated aortic...
Objective: HDL (high-density lipoprotein) particles are known to possess several antiatherogenic properties that include the removal of excess cholesterol from peripheral tissues, maintenance endothelial integrity, antioxidant, and anti-inflammatory activities. ApoA-I overexpression in apoE-deficient (EKO) mice has been shown increase levels strongly reduce atherosclerosis development. The aim study was investigate gene expression patterns associated with development aorta EKO how plasma...
Summary Sphingosine 1-phosphate (S1P), a lysosphingolipid associated with high-density lipoprotein (HDL), contributes to the anti-atherogenic potential attributed this lipoprotein. This study examined whether reduction of S1P plasma levels affects atherosclerosis in murine model disease. LDL-R−/−mice on Western diet were given ABC294640, an inhibitor sphingosine kinase (SphK) for 16 weeks. ABC294640 decreased by approximately 30%. However, failed affect atherosclerotic lesion formation....
Abstract Clusterin (CLU) is a chaperone‐like protein with multiple functions. sCLU frequently upregulated in prostate tumor cells after chemo‐ or radiotherapy and surgical pharmacological castration. Moreover, CLU has been documented to modulate the cellular homolog of murine thymoma virus akt8 oncogene (AKT) activity. Here, we investigated how overexpression influences phosphatidylinositol 3′‐kinase (PI3K)/AKT signaling human normal cancer epithelial cells. Human stably transfected were...