A5003215848- Ovarian function and disorders
- Reproductive Biology and Fertility
- Reproductive Health and Technologies
- Sperm and Testicular Function
- Hypothalamic control of reproductive hormones
- Assisted Reproductive Technology and Twin Pregnancy
- Estrogen and related hormone effects
- Sexual Differentiation and Disorders
- Hormonal and reproductive studies
- Growth Hormone and Insulin-like Growth Factors
- Health Systems, Economic Evaluations, Quality of Life
- Amino Acid Enzymes and Metabolism
- Reproductive Physiology in Livestock
- Sexual function and dysfunction studies
- Healthcare cost, quality, practices
- Effects and risks of endocrine disrupting chemicals
- Glycosylation and Glycoproteins Research
- Lung Cancer Diagnosis and Treatment
- PI3K/AKT/mTOR signaling in cancer
- Peroxisome Proliferator-Activated Receptors
- Insurance, Mortality, Demography, Risk Management
- Pancreatic function and diabetes
- Hormonal Regulation and Hypertension
- Monoclonal and Polyclonal Antibodies Research
- Food Allergy and Anaphylaxis Research
Merck (Germany)
2017-2024
University of Modena and Reggio Emilia
2020-2024
Instituto Tropical de Medicina Reprodutiva
2020
Universidade Federal de São Paulo
2020
Merck Serono (Italy)
2009-2019
Karolinska University Hospital
2011
Amsterdam UMC Location University of Amsterdam
2011
Mitera Hospital
2011
Reinier de Graaf Hospital
2011
Tel Aviv University
2011
Human luteinizing hormone (hLH) and chorionic gonadotropin (hCG) act on the same receptor (LHCGR) but it is not known whether they elicit cellular molecular response. This study compares for first time activation of cell-signalling pathways gene expression in response to hLH hCG. Using recombinant hCG we evaluated kinetics cAMP production COS-7 hGL5 cells permanently expressing LHCGR (COS-7/LHCGR, hGL5/LHCGR), as well cAMP, ERK1/2, AKT progesterone primary human granulosa (hGLC). The...
Premature ovarian failure and female infertility are frequent side effects of anticancer therapies, owing to the extreme sensitivity reserve oocytes damaging irradiation chemotherapy on DNA. We report here a robust protective effect luteinizing hormone (LH) primordial follicle pool prepubertal ovaries against cisplatin (Cs)-induced apoptosis. In vitro LH treatment fragments generated anti-apoptotic signals by subset somatic cells expressing receptor (LHR) through cAMP/PKA Akt pathways. Such...
Classically, follicle-stimulating hormone receptor (FSHR)-driven cAMP-mediated signaling boosts human ovarian follicle growth and oocyte maturation. However, contradicting in vitro data suggest a different view on physiological significance of FSHR-mediated cAMP signaling. We found that the G-protein-coupled estrogen (GPER) heteromerizes with FSHR, reprogramming cAMP/death signals into proliferative stimuli fundamental for sustaining survival. In granulosa cells, survival are missing at high...
Background and objectives: Male infertility is a global health dilemma Follicle-Stimulating Hormone (FSH) administration has shown promising results. Several studies showed that infertile men with normal semen parameters have low levels of DNA damage while abnormal more at the level. Sperm may affect reproductive outcome been associated failure in achievement competent embryos pregnancy fulfillment. The aim this study was to evaluate whether recombinant FSH (Gonal-f® PEN 900 IU) could...
Various preparations of follicle-stimulating hormone (FSH) are commercially available; however, they differ in glycoforms composition and purity owing to their respective sources. Additional chemical/physical changes can also be introduced during manufacturing impact biological activity (biopotency), which is routinely assessed using an vivo bioassay (Steelman-Pohley). This study aimed determine whether vitro could assess biopotency by distinguishing between r-hFSH variants with similar...
Although the full primary structures of alfa and beta subunits reference r-hFSH-alfa its biosimilars are identical, cell context-dependent differences in expressing lines manufacturing process can lead to variations glycosylation profiles. In present study, we compared structural features with those five biosimilar preparations approved different global regions outside Europe (Primapur®, Jin Sai Heng®, Follitrope®, Folisurge®, Corneumon®) respect glycosylation, macro- microheterogeneity,...
The canonical androgen synthesis in Leydig cells involves Δ5 and Δ4 steroids. Besides, the backdoor pathway, eompassing 5α 5α,3α steroids, is gaining interest fetal adult pathophysiology. Moreover, role of epimers progesterone metabolites still unknown. We developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for measuring 20 steroids used it to investigate steroid secretion induced by human chorionic gonadotropin (hCG) mouse tumor cell line 1 (mLTC1). Steroids were...
Background This study aimed to confirm that the incremental dose/clicks system dispenses accurate doses for Merck family of fertility pen injectors.
Controlled ovarian stimulation (COS) leading to ovulation of multiple follicles is a crucial aspect biomedical infertility care. Nevertheless, biomarkers useful for COS management are still lacking. Peroxisome proliferator-activated receptors (PPARs) nuclear hormone relevant steroid metabolism in granulosa cells (GCs). We investigated whether PPARs and their steroidogenic targets were differentially expressed GCs differentiated under different recombinant or urinary gonadotropin...
Purpose: Although a robust physiological rationale supports follicle stimulating hormone (FSH) use in male idiopathic infertility, useful biomarkers to evaluate its efficacy are not available. Thus, the primary aim of study was if testosterone serum levels related sperm DNA fragmentation (sDF) index change after FSH administration. The secondary confirm sDF validity as biomarker administration effectiveness infertility. Methods: A retrospective, post-hoc re-analysis performed on...
Background/Aims: FSH receptor (FSHR) Ala307Thr and Asn680Ser LHβ chain (LHB) Trp28Arg Ile35Thr polymorphisms affect the response to pharmacological ovarian stimulation with r-FSH in women undergoing assisted reproductive treatment (ART). Here, we evaluated expression level of selected genes involved follicle maturation possible onset apoptosis cumulus cells patients single double FSHR LHB polymorphisms, as potential markers oocyte competence. Methods: Cumulus from 36 stimulated were...
Although biosimilar drugs may be cheaper to purchase than reference biological products, they not the most cost-effective treatment achieve a desired outcome. The analysis reported here compared overall costs live birth using follitropin alfa (GONAL-f) or (Ovaleap) in Spain, Italy and Germany.Patient data was obtained from published sources; assisted-reproductive technology, gonadotropin, follow-up adverse-event-related were calculated tariffs reimbursement frameworks for each country....
This was a retrospective real-world evidence analysis of the costs per live birth for reference recombinant human follicle-stimulating hormone alfa (r-hFSH-alfa) versus highly purified urinary menopausal gonadotropin (hMG-HP), based on data from German in vitro fertilization registry (RecDate). Pregnancy and rates RecDate study over three complete assisted reproductive technology (ART) cycles using same drug were used as clinical inputs. Costs related to ART treatment drugs obtained public...