A5004911952- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Photosynthetic Processes and Mechanisms
- Nuclear Structure and Function
- Plant nutrient uptake and metabolism
- Reproductive Biology and Fertility
- DNA and Biological Computing
- Peptidase Inhibition and Analysis
- Plant Reproductive Biology
- DNA Repair Mechanisms
- Cellular transport and secretion
- Histone Deacetylase Inhibitors Research
- RNA Research and Splicing
- Genomic variations and chromosomal abnormalities
- Plant Molecular Biology Research
- Protist diversity and phylogeny
- Chromosomal and Genetic Variations
- Advanced Fluorescence Microscopy Techniques
- Ubiquitin and proteasome pathways
- Glioma Diagnosis and Treatment
- Genetic and Kidney Cyst Diseases
- Advanced Electron Microscopy Techniques and Applications
Universidade do Porto
2019-2024
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2019-2022
MRC Laboratory of Molecular Biology
2015-2016
Medical Research Council
2016
University of Liverpool
2011-2015
Physiological Society
2011
Microtubule-associated proteins of the mitotic spindle are thought to be important for initial assembly and maintenance structure function. However, distinguishing roles a given protein is difficult. Most experimental methods inactivation slow therefore affect both maintenance. Here, we have used 'knocksideways' rapidly (∼5 minutes) specifically remove TACC3-ch-TOG-clathrin non-motor complexes from kinetochore fibers (K-fibers). This method allows complex inactivated at defined stages...
Abstract An egg must be fertilized by a single sperm only. To prevent polyspermy, the zona pellucida, structure that surrounds mammalian eggs, becomes impermeable upon fertilization, preventing entry of further sperm. The structural changes in fertilization are driven exocytosis cortical granules. These translocate from oocyte’s centre to plasma membrane during meiosis. However, very little is known about mechanism granule translocation. Here we investigate transport and dynamics live...
// Joseph J. Sacco 1 , Jenna Kenyani Zohra Butt Rachel Carter Hui Yi Chew 1, 2 Liam P. Cheeseman 3 Sarah Darling Michael Denny Sylvie Urbé Clague Judy M. Coulson Cellular & Molecular Physiology, Institute of Translational Medicine, University Liverpool, UK Current address: Cancer Stem Cell Biology, Agency for Science Technology and Research, Genome Singapore, Singapore MRC Laboratory Cambridge, Correspondence to: Coulson, e-mail: j.m.coulson@liv.ac.uk Keywords: histone deacetylase...
According to the prevailing 'clock' model, chromosome decondensation and nuclear envelope reformation when cells exit mitosis are byproducts of Cdk1 inactivation at metaphase-anaphase transition, controlled by spindle assembly checkpoint. However, mitotic was recently shown be a function separation during anaphase, assisted midzone Aurora B phosphorylation gradient - 'ruler' model. Here we found that remains active anaphase due ongoing APC/CCdc20- APC/CCdh1-mediated degradation B-type...
Chromosome segregation in mammals relies on the maturation of a thick bundle kinetochore-attached microtubules known as k-fiber. How k-fibers mature from initial kinetochore microtubule attachments remains fundamental question. By combining molecular perturbations and phenotypic analyses Indian muntjac fibroblasts containing lowest diploid chromosome number (2N = 6) distinctively large kinetochores, with fixed/live-cell super-resolution coherent-hybrid stimulated emission depletion (CH-STED)...
Accurate chromosome segregation during mitosis is achieved by the kinetochore fibres (K-fibres) of spindle apparatus. These fibers are bundles microtubules (MTs) connected non-motor bridges. We recently identified a TACC3/ch-TOG/clathrin complex that constitutes shortest class inter-MT bridge in K-fibers. TACC3 anchors to MTs and this dependent on phosphorylation Aurora A kinase. Here we show inhibition kinase using MLN8237 results 1) loss clathrin from spindles, 2) destabilization K-fibers...
Chromosomes and their constituent compartments are core elements of the cell division machinery. In mammalian oocytes, defects in structure or composition chromosomes a leading cause aberrant failed meiosis, by extension, can miscarriage infertility. The underlying mechanisms poorly understood, but critical for development novel diagnostics treatments. chromosome periphery, least understood compartment, has recently emerged as an essential component mitotic somatic division. However, female...
Abstract Chromosome segregation in mammals relies on the maturation of a thick bundle kinetochore-attached microtubules known as k-fibers. How k-fibers mature from initial kinetochore-microtubule attachments remains fundamental question. By combining functional perturbations Indian muntjac, placental mammal with lowest chromosome number (n=3) and distinctively large kinetochores, fixed- subsecond live-cell super-resolution CH-STED nanoscopy, we uncovered mechanism by which Augmin mediates...
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Summary According to the prevailing “clock” model, chromosome decondensation and nuclear envelope reassembly during mitotic exit are byproducts of Cdk1 inactivation at metaphase-anaphase transition, controlled by spindle assembly checkpoint. However, was recently shown be a function separation anaphase, assisted midzone Aurora B phosphorylation gradient - “ruler” model. Here we reconciled both models showing that Cyclin B1 degradation continues anaphase in Drosophila , mouse human cells,...