A5026694980- Microtubule and mitosis dynamics
- Fungal and yeast genetics research
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- Cellular Mechanics and Interactions
- RNA Interference and Gene Delivery
- DNA Repair Mechanisms
- Plant nutrient uptake and metabolism
- Protein Structure and Dynamics
- CRISPR and Genetic Engineering
- Retinal Development and Disorders
- Biofuel production and bioconversion
- RNA modifications and cancer
- Bacteriophages and microbial interactions
- Mitochondrial Function and Pathology
- Neuroscience and Neuropharmacology Research
- Advanced Electron Microscopy Techniques and Applications
- Agriculture and Farm Safety
- Epigenetics and DNA Methylation
- Micro and Nano Robotics
- Genomics and Chromatin Dynamics
- Enzyme Structure and Function
- DNA and Nucleic Acid Chemistry
- Protist diversity and phylogeny
University of Washington
2010-2021
Scripps College
2009
Structural features of neurons create challenges for effective production and distribution essential metabolic energy. We investigated how energy is distributed between cellular compartments in photoreceptors. In avascular retinas, aerobic occurs only mitochondria that are located centrally within the photoreceptor. Our findings indicate flows from these central as phosphocreatine toward photoreceptor’s synaptic terminal darkness. light, it opposite direction ATP outer segment. Consistent...
The CSB-PGBD3 fusion protein arose more than 43 million years ago when a 2.5-kb piggyBac 3 (PGBD3) transposon inserted into intron 5 of the Cockayne syndrome Group B (CSB) gene in common ancestor all higher primates. As result, full-length CSB is now coexpressed with an abundant by alternative splicing exons 1–5 to PGBD3 transposase. An internal deletion transposase ORF also gave rise 889 dispersed, 140-bp MER85 elements that were mobilized trans binds MER85s vitro and induces strong...
During mitosis, kinetochores physically link chromosomes to the dynamic ends of spindle microtubules. This linkage depends on Ndc80 complex, a conserved and essential microtubule-binding component kinetochore. As member protein forms microtubule attachments through calponin homology domain. is also required for recruiting other components kinetochore responding mitotic regulatory signals. While domain has been focus biochemical structural characterization, function remainder poorly...
Centrosomes, or spindle pole bodies (SPBs) in yeast, are vital mechanical hubs that maintain load-bearing attachments to microtubules during mitotic assembly, positioning, and chromosome segregation. However, the strength of microtubule-centrosome is unknown, possibility force might regulate centrosome function has scarcely been explored. To uncover how centrosomes sustain force, we purified SPBs from budding yeast used laser trapping manipulate single attached vitro. Our experiments reveal...
Phosphorylation regulates yeast spindle pole body (SPB) duplication and separation likely microtubule nucleation. We report a phosphoproteomic analysis using tandem mass spectrometry of enriched Saccharomyces cerevisiae SPBs for two cell cycle arrests, G1/S the mitotic checkpoint, expanding on previously reported data sets. present novel state arrested in by cdc4-1 temperature sensitive mutation, with particular focus phosphorylation events γ-tubulin small complex (γ-TuSC). The arrest is...
Fourier transform infrared (FTIR) spectroscopy and a library of FTIR marker bands have been used to examine the structure relative flexibilities conferred by different flanking sequences on EcoRI binding site. This approach allowed us unique peaks subtle changes in spectra d(AAAGAATTCTTT)2, d(TTCGAATTCGAA)2, d(CGCGAATTCGCG)2 thereby identify local base pairing, stacking, backbone conformation, glycosidic bond rotation, sugar puckering studied sequences. The induce differences puckers,...
To assemble a bipolar spindle, microtubules emanating from two poles must bundle into an antiparallel midzone, where plus end–directed motors generate outward pushing forces to drive pole separation. Midzone cross-linkers and display only modest preferences for filaments, duplicated are initially tethered together, arrangement that instead favors parallel interactions. Pivoting of around spindle might help overcome this geometric bias, but the intrinsic pivoting flexibility microtubule–pole...
ABSTRACT Phosphorylation regulates yeast spindle pole body (SPB) duplication and separation likely microtubule nucleation. We report a phosphoproteomic analysis using tandem mass spectrometry of purified Saccharomyces cerevisiae SPBs for two cell cycle arrests, G1/S the mitotic checkpoint, expanding on previously reported data sets. present novel state arrested in by cdc4-1 temperature sensitive mutation, with particular interest phosphorylation events γ-tubulin small complex (γ-TuSC). The...