A5052893976- Enzyme Production and Characterization
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Peptidase Inhibition and Analysis
- Plant Stress Responses and Tolerance
- Enzyme Structure and Function
- Plant-Microbe Interactions and Immunity
- Protein Hydrolysis and Bioactive Peptides
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Genomics and Phylogenetic Studies
- Plant biochemistry and biosynthesis
- Plant Pathogens and Fungal Diseases
- Histone Deacetylase Inhibitors Research
- Bacterial Genetics and Biotechnology
- Plant tissue culture and regeneration
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Biochemical and Molecular Research
- Biochemical and Structural Characterization
- Plant Reproductive Biology
- Microbial Community Ecology and Physiology
Northwest A&F University
2025
Southern Medical University
2025
Hefei University of Technology
2014-2024
Wuhan University
2015-2024
Center of Hubei Cooperative Innovation for Emissions Trading System
2014-2024
Nanchang University
2015-2024
Third Affiliated Hospital of Nanchang University
2018-2024
Southwest University
2018-2024
Central South University
2018-2023
Zhejiang University
2018-2023
The kiwifruit (Actinidia chinensis) is an economically and nutritionally important fruit crop with remarkably high vitamin C content. Here we report the draft genome sequence of a heterozygous kiwifruit, assembled from ~140-fold next-generation sequencing data. has total length 616.1 Mb contains 39,040 genes. Comparative genomic analysis reveals that undergone ancient hexaploidization event (γ) shared by core eudicots two more recent whole-genome duplication events. Both events occurred...
Cadmium (Cd) is an environmental pollutant with high toxicity to animals and plants. It has been established that the glutathione (GSH)-dependent phytochelatin (PC) synthesis pathway one of most important mechanisms contributing Cd accumulation tolerance in However, transcription factors involved regulating GSH-dependent PC remain largely unknown. Here, we identified Arabidopsis (Arabidopsis thaliana) Cd-resistant mutant xcd2-D (XVE system-induced cadmium-tolerance2) using a forward genetics...
Cadmium (Cd) is one of the most dangerous environmental pollutants among heavy metals, and threatens food safety human health by accumulating in plant sink tissues. Here, we report a novel regulatory cascade that profoundly influences Cd tolerance Arabidopsis. Phenotypic analysis showed an insertional knockdown mutation at Arabidopsis Tóxicos en Levadura 31 (ATL31) locus resulted hypersensitivity to stress, likely due significant increase accumulation. Consistently, ATL31-overexpressing...
Abstract Pentatricopeptide repeat (PPR) proteins, which are characterized by tandem 30–40 amino acid sequence motifs, constitute of a large gene family in plants. Some PPR proteins have been identified to play important roles organellar RNA metabolism and organ development Arabidopsis rice. However, functions genes woody species remain largely unknown. Here, we total 626 containing motifs the Populus trichocarpa genome. A comprehensive genome-wide analysis poplar was performed, including...
Kiwifruit (Actinidia spp.) is a dioecious plant with fruits containing abundant vitamin C and minerals. A handful of kiwifruit species have been domesticated, among which Actinidiaeriantha increasingly favored in breeding owing to its superior commercial traits. Recently, elite cultivars from A. eriantha successfully selected further studies on their biology potential require genomic information, currently unavailable.We assembled chromosome-scale genome sequence cultivar White using...
Kiwifruit (Actinidia spp.) plants produce economically important fruits containing abundant, balanced phytonutrients with extraordinarily high vitamin C contents. Since the release of first kiwifruit reference genome sequence in 2013, large volumes and transcriptome data have been rapidly accumulated for a handful species. To efficiently store, analyze, integrate, disseminate these large-scale datasets to research community, we constructed Genome Database (KGD; http://kiwifruitgenome.org/)....
Controlled stability of proteins is a highly efficient mechanism to direct diverse processes in plants. A key regulatory system for protein given by the CULLIN-RING E3 ligases (CRLs). In this work, MYB43 identified as novel target CUL4-DDB1-PRL1 (PLEIOTROPIC REGULATORY LOCUS 1)-RING ligase (CRL4PRL1 ligase). Its depends on presence PRL1, WD40-containing functioning substrate receptor CRL4 ligases. Genetic studies have indicated that negative regulator cadmium (Cd) tolerance Arabidopsis...
BackgroundFor patients with early-stage breast cancers, neoadjuvant treatment is recommended for non-luminal tumors instead of luminal tumors. Preoperative distinguish between and cancers at early stages will facilitate decisions making. However, the molecular immunohistochemical subtypes based on biopsy specimens are not always consistent final results surgical due to high intra-tumoral heterogeneity. Given that, we aimed develop validate a deep learning radiopathomics (DLRP) model...
CULLIN4-RING ubiquitin ligases (CRL4s) as well their targets are fundamental regulators functioning in many key developmental and stress responses eukaryotes. In tomato (Solanum lycopersicum), molecular cloning has revealed that the underlying genes of natural spontaneous mutations high pigment 1 (hp1), 2 (hp2) uniform ripening (u) encode UV-DAMAGED DNA BINDING PROTEIN (DDB1), DE-ETIOLATED (DET1) GOLDEN 2-LIKE (GLK2), respectively. However, basis opposite actions GLK2 vs CUL4-DDB1-DET1...
Chrysanthemum morifolium is an ornamentally and medicinally important plant species. Up to date, molecular genetic investigations have largely focused on determination of flowering time in the ornamental However, little known about gene regulatory networks for biosynthesis flavonoids medicinal In current study, we employed high-throughput sequencing technology profile genome-wide transcriptome C. 'Chuju', a famous species traditional Chinese medicine. A total 63,854 unigenes with average...
Long noncoding RNAs (lncRNAs) can regulate the progression of DN. This research aimed to study effect lncRNA KCNQ1OT1 on oxidative stress and pyroptosis renal tubular epithelial cells induced by high glucose (HG).RT-qPCR analysis detected expression in serum with DN HG-induced HK-2 cells, detect NLRP3, cleaved-caspase1, P-caspase1, IL-1β, p-IL-1β GSDMD-N confirm transfection effects. The was also analyzed Western blot analysis. ELISA assay levels TNF-α, IL-6 MCP-1. ROS, MDA SOD were...
Summary Anthocyanins are involved in several aspects of development and defence poplar ( Populus spp.). Although, over the past decades, significant progress has been made uncovering these anthocyanin biosynthetic regulatory mechanisms, fundamental understanding epigenetic regulation this pathway is still largely unclear. Here, we isolated a histone H3K9 demethylase gene JMJ25 from characterized its role biosynthesis by genetic biochemical approaches. was induced continuous dark treatment....
Abstract It has been reported that lncRNA PANDAR (promoter of CDKN1A antisense DNA damage-activated RNA) is induced as a result damage, and it regulates the reparation damage. In this study, we investigated role in progression breast cancer found was up-regulated tissues cell lines. The knockdown suppresses G1/S transition cells. We demonstrated mechanistically regulation by partly due to transcriptional modulation p16 INK4A . Moreover, showed impacted expression regulating recruitment Bmi1...
Long non-coding RNA (lncRNA) SNHG5 has been found to play an important role in tumors. Nevertheless, the function and mechanism of lncRNA osteosarcoma (OS) remains unclear. The purpose this study was investigate whether can regulate occurrence development OS cells. We performed quantitative real time PCR detect expression 143B, MG63 (knockdown) U2OS, U2R (overexpression) cell lines were chosen for SNHG5. effect SNHG5, miR-212-3p, SGK3 cells explored by MTT assays, clony formation, flow...
Previous discovering meticulously illustrates the post-translational modifications and protein stability regulation of ICE1 their role in cold stress. However, studies on interaction with other transcription factors, function modulation stress tolerance, as well transition between growth are largely insufficient. In this work, we found that maltose binding (MBP) 43 directly binds to promoters CBF genes repress expression, thereby negatively regulating freezing tolerance. Biochemical genetic...
Abstract Background Extensive studies have demonstrated that the COBRA gene is critical for biosynthesis of cell wall constituents comprising structural tissues roots, stalks, leaves and other vegetative organs, however, its role in fruit development ripening remains largely unknown. Results We identified a tomato ( SlCOBRA-like ) homologous to Arabidopsis , determined fleshy biology. The highly expressed organs early development, but expression declines dramatically during stages, implying...
CD147 contributes to increased aerobic glycolysis through which it promotes tumor growth. Accumulating evidence suggests that exerts a variety of functions in thyroid cancer (TC) progression but the molecular mechanisms and therapeutic value remain unclear.CD147 levels TC tissues were analyzed assess its relationship with prognosis disease progression. A microRNA (miRNA) microarray bioinformatics approach used identify regulators measurement expression these miRNAs cell lines. Precursor...
Plants utilize energy from sunlight to perform photosynthesis in chloroplast, an organelle that could be damaged by solar UV radiation. The ultraviolet-B (UV-B) photoreceptor UVR8 is required for UV-B perception and signal transduction. However, little known about how influence chloroplast development under Here, we characterized tomato gene (SlUVR8) our results indicated SlUVR8 facilitate plant acclimation stress orchestrating expression of the UVB-responsive genes (HY5 CHS) accumulating...
Biological control of postharvest diseases has been proven to be an effective alternative chemical control. As environmentally friendly biocontrol agent, Bacillus subtilis widely applied. This study explores its application in kiwifruit soft rot and reveals the corresponding mechanisms. Treatment with cell-free supernatant (CFS) BS-1 significantly inhibits mycelial growth pathogen Botryosphaeria dothidea attenuates pathogenicity on a concentration-dependent manner. In particular, diameter...
Pyrolysin is an extracellular subtilase produced by the marine hyperthermophilic archaeon Pyrococcus furiosus. This enzyme functions at high temperatures in seawater, but little known about effects of metal ions on properties pyrolysin. Here, we report that supplementation Na(+), Ca(2+), or Mg(2+) salts concentrations similar to those seawater destabilizes recombinant pyrolysin leads increase activity. The destabilizing effect appears be related disturbance surface electrostatic interactions...
Background . Sorafenib is a multi-target kinase inhibitor that has been approved as unique target drug for the treatment of advanced hepatocellular carcinoma (HCC). However, due to frequent occurrence resistance, its efficacy often limited. The aim this study was explore function HOX transcript antisense intergenic RNA (HOTAIR) HCC with sorafenib, and underlying mechanism. Methods A cell counting kit-8 (CCK-8) assay Edu were used examine viability proliferation cells. Quantitative real-time...
The biosynthesis of catechins, a major type flavonoids accumulated in tea, is mediated by developmental cues and environmental stimuli. Light enhances but shading treatment reduces catechin accumulation tea leaves. However, the transcription factors involved light-mediated remain to be identified. Two GOLDEN2 LIKE genes from plant (CsGLK1 CsGLK2) were isolated characterized both tomato plants. Transcripts CsGLK1 CsGLK2 affected light intensity Overexpression promoted chloroplast development...